Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms
The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM...
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
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2011
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03257541_v43_n3_p212_Lasagno https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03257541_v43_n3_p212_Lasagno_oai |
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I28-R145-paper_03257541_v43_n3_p212_Lasagno_oai2024-08-16 Lasagno, M.C. Reinoso, E.B. Dieser, S.A. Calvinho, L.F. Buzzola, F. Vissio, C. Bogni, C.I. Odierno, L.M. 2011 The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. The S. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profiles were identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similarity were identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberis strains with the same PFGE pattern showed different virulence profiles. Bovine S. uberis strains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypic characteristics, and the PFGE patterns are not associated with the virulence profiles. Fil:Buzzola, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03257541_v43_n3_p212_Lasagno eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Rev. Argent. Microbiol. 2011;43(3):212-217 Bovine subclinical mastitis Pulsed-field gel electrophoresis Streptococcus uberis Virulence factors cyclic AMP hyaluronidase plasminogen activator Argentina article bacterial membrane bacterial virulence bacterium isolation bovine mastitis dairying genetic heterogeneity genotype milk nonhuman phenotype Streptococcus uberis Animal Husbandry Animals Argentina Asymptomatic Infections Bacterial Capsules Bacterial Proteins Bacterial Typing Techniques Cattle Dairying DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Female Genotype Hemolysin Proteins Hyaluronoglucosaminidase Mastitis, Bovine Phenotype Streptococcal Infections Streptococcus Virulence Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03257541_v43_n3_p212_Lasagno_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
Bovine subclinical mastitis Pulsed-field gel electrophoresis Streptococcus uberis Virulence factors cyclic AMP hyaluronidase plasminogen activator Argentina article bacterial membrane bacterial virulence bacterium isolation bovine mastitis dairying genetic heterogeneity genotype milk nonhuman phenotype Streptococcus uberis Animal Husbandry Animals Argentina Asymptomatic Infections Bacterial Capsules Bacterial Proteins Bacterial Typing Techniques Cattle Dairying DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Female Genotype Hemolysin Proteins Hyaluronoglucosaminidase Mastitis, Bovine Phenotype Streptococcal Infections Streptococcus Virulence |
| spellingShingle |
Bovine subclinical mastitis Pulsed-field gel electrophoresis Streptococcus uberis Virulence factors cyclic AMP hyaluronidase plasminogen activator Argentina article bacterial membrane bacterial virulence bacterium isolation bovine mastitis dairying genetic heterogeneity genotype milk nonhuman phenotype Streptococcus uberis Animal Husbandry Animals Argentina Asymptomatic Infections Bacterial Capsules Bacterial Proteins Bacterial Typing Techniques Cattle Dairying DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Female Genotype Hemolysin Proteins Hyaluronoglucosaminidase Mastitis, Bovine Phenotype Streptococcal Infections Streptococcus Virulence Lasagno, M.C. Reinoso, E.B. Dieser, S.A. Calvinho, L.F. Buzzola, F. Vissio, C. Bogni, C.I. Odierno, L.M. Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms |
| topic_facet |
Bovine subclinical mastitis Pulsed-field gel electrophoresis Streptococcus uberis Virulence factors cyclic AMP hyaluronidase plasminogen activator Argentina article bacterial membrane bacterial virulence bacterium isolation bovine mastitis dairying genetic heterogeneity genotype milk nonhuman phenotype Streptococcus uberis Animal Husbandry Animals Argentina Asymptomatic Infections Bacterial Capsules Bacterial Proteins Bacterial Typing Techniques Cattle Dairying DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Female Genotype Hemolysin Proteins Hyaluronoglucosaminidase Mastitis, Bovine Phenotype Streptococcal Infections Streptococcus Virulence |
| description |
The aim of this study was to investigate the phenotypic and genotypic characteristics of Streptococcus uberis isolated from subclinical mastitis (SCM) cases, and to examine the possible association between both characteristics. A total of 32 S. uberis were isolated from 772 quarter milk samples (SCM > 250,000 cells/ml) collected from 195 cows selected randomly from 18 dairy farms located in Argentina. The S. uberis strains were characterized phenotypically by the presence of virulence factors as plasminogen activator factor (PAF), hyaluronidase (HYA), capsule (CAP) and CAMP factor, and were further characterized genotypically by pulsed-field gel electrophoresis (PFGE). S. uberis strains expressed plasminogen activator factor, hyaluronidase or capsule (65.5 %, 56.3 %, 59.4 %, respectively), but only 25 % of isolates were CAMP factor positive. Thirteen different virulence profiles were identified on the basis of the combination of virulence factors. Eighteen PFGE patterns with 90% of similarity were identified among 32 S. uberis. A great diversity of virulence profiles and PFGE patterns were present among dairy farms. S. uberis strains with the same PFGE pattern showed different virulence profiles. Bovine S. uberis strains causing SCM included in the present study showed heterogeneity in regard to their phenotypic and genotypic characteristics, and the PFGE patterns are not associated with the virulence profiles. |
| format |
Artículo Artículo publishedVersion |
| author |
Lasagno, M.C. Reinoso, E.B. Dieser, S.A. Calvinho, L.F. Buzzola, F. Vissio, C. Bogni, C.I. Odierno, L.M. |
| author_facet |
Lasagno, M.C. Reinoso, E.B. Dieser, S.A. Calvinho, L.F. Buzzola, F. Vissio, C. Bogni, C.I. Odierno, L.M. |
| author_sort |
Lasagno, M.C. |
| title |
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms |
| title_short |
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms |
| title_full |
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms |
| title_fullStr |
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms |
| title_full_unstemmed |
Phenotypic and genotypic characterization of Streptococcus uberis isolated from bovine subclinical mastitis in Argentinean dairy farms |
| title_sort |
phenotypic and genotypic characterization of streptococcus uberis isolated from bovine subclinical mastitis in argentinean dairy farms |
| publishDate |
2011 |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v43_n3_p212_Lasagno https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03257541_v43_n3_p212_Lasagno_oai |
| work_keys_str_mv |
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