Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants

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arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this w...

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Autores principales:	Ruiz, J.A., Fernández, R.O., Nikel, P.I., Méndez, B.S., Pettinari, M.J.
Formato:	Artículo publishedVersion
Publicado:	2006
Materias:	arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03781097_v258_n1_p55_Ruiz https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v258_n1_p55_Ruiz_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_03781097_v258_n1_p55_Ruiz_oai
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spelling	I28-R145-paper_03781097_v258_n1_p55_Ruiz_oai2024-08-16 Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J. 2006 arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_03781097_v258_n1_p55_Ruiz info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEMS Microbiol. Lett. 2006;258(1):55-60 arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v258_n1_p55_Ruiz_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli
spellingShingle	arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J. Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
topic_facet	arcA O2 consumption PHB ROS Toluidine blue poly(3 hydroxybutyric acid) tolonium chloride aerobic metabolism article controlled study Escherichia coli gene mutation genetic code molecular dynamics nonhuman oxidation reduction reaction oxygen consumption phenotype polymerization priority journal Bacterial Outer Membrane Proteins Escherichia coli Escherichia coli Proteins Hydroxybutyrates Mutation Oxygen Consumption Phenotype Polyesters Reactive Oxygen Species Recombination, Genetic Repressor Proteins Arca Escherichia coli
description	arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
format	Artículo Artículo publishedVersion
author	Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J.
author_facet	Ruiz, J.A. Fernández, R.O. Nikel, P.I. Méndez, B.S. Pettinari, M.J.
author_sort	Ruiz, J.A.
title	Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
title_short	Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
title_full	Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
title_fullStr	Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
title_full_unstemmed	Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants
title_sort	dye( arc)mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in escherichia coli recombinants
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_03781097_v258_n1_p55_Ruiz https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_03781097_v258_n1_p55_Ruiz_oai
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Dye( arc)mutants: Insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants

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