Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry

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Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section...

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Autores principales:	Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., Hiraoka, K.
Formato:	Artículo publishedVersion
Publicado:	2009
Materias:	Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10440305_v20_n12_p2304_Yu https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v20_n12_p2304_Yu_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_10440305_v20_n12_p2304_Yu_oai
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spelling	I28-R145-paper_10440305_v20_n12_p2304_Yu_oai2024-08-16 Yu, Z. Chen, L.C. Suzuki, H. Ariyada, O. Erra-Balsells, R. Nonami, H. Hiraoka, K. 2009 Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems. © 2009. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_10440305_v20_n12_p2304_Yu info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar J. Am. Soc. Mass Spectrom. 2009;20(12):2304-2311 Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v20_n12_p2304_Yu_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa
spellingShingle	Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa Yu, Z. Chen, L.C. Suzuki, H. Ariyada, O. Erra-Balsells, R. Nonami, H. Hiraoka, K. Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
topic_facet	Bioactive compounds Carbohydrate content Direct analysis Direct profiling Electrospray ionization mass spectrometry Electrosprays Flower buds In-vivo Metabolic changes Phytochemicals Sample consumption Sample preparation Time-of-flight mass spectrometers Amines Amino acids Biological systems Carbohydrates Ionization Mass spectrometers Mass spectrometry Organic acids Particle detectors Probes Vegetation Electrospray ionization amino acid anthocyanin carbohydrate pelargonidin plant medicinal product amino acid analysis article carbohydrate analysis controlled study drug structure electrospray mass spectrometry flower foliage in vivo study nonhuman petal plant bulb plant growth plant leaf plant tissue time of flight mass spectrometry tulip Carbohydrates Plant Roots Spectrometry, Mass, Electrospray Ionization Transducers Tulipa Tulipa
description	Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems. © 2009.
format	Artículo Artículo publishedVersion
author	Yu, Z. Chen, L.C. Suzuki, H. Ariyada, O. Erra-Balsells, R. Nonami, H. Hiraoka, K.
author_facet	Yu, Z. Chen, L.C. Suzuki, H. Ariyada, O. Erra-Balsells, R. Nonami, H. Hiraoka, K.
author_sort	Yu, Z.
title	Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
title_short	Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
title_full	Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
title_fullStr	Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
title_full_unstemmed	Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry
title_sort	direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry
publishDate	2009
url	http://hdl.handle.net/20.500.12110/paper_10440305_v20_n12_p2304_Yu https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v20_n12_p2304_Yu_oai
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Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry

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