Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay

Repellents serve an important role in bite protection. Tick repellents largely rely on biomechanisms that induce responses with direct contact, but synthetic pyrethroids used as spatial repellents against insects have received recent attention for potential use in tick protection systems. An in vitr...

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Autores principales: Siegel, Eric L., Olivera, Marcos, Martínez Roig, Esteban, Perry, Melynda, Li, Andrew Y., D'hers, Sebastián, Elman, Noel M., Rich, Stephen M.
Formato: Artículo de Publicación Periódica
Lenguaje:Inglés
Publicado: Plos One 2023
Materias:
REPELENTES
INSECTOS
Acceso en línea:https://ri.itba.edu.ar/handle/123456789/4192
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Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA) de Instituto Tecnológico de Buenos Aires (ITBA)
id I32-R138-123456789-4192
record_format dspace
spelling I32-R138-123456789-41922023-06-24T03:01:23Z Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay Siegel, Eric L. Olivera, Marcos Martínez Roig, Esteban Perry, Melynda Li, Andrew Y. D'hers, Sebastián Elman, Noel M. Rich, Stephen M. REPELENTES INSECTOS Repellents serve an important role in bite protection. Tick repellents largely rely on biomechanisms that induce responses with direct contact, but synthetic pyrethroids used as spatial repellents against insects have received recent attention for potential use in tick protection systems. An in vitro vertical climb assay was designed to assess spatial repellency against Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis adult, female ticks. Climbing behavior was assessed with and without the presence of two spatial repellents, transfluthrin and metofluthrin. Repellency parameters were defined to simulate the natural questing behavior of ambushing ticks, including measures of detachment, pseudo-questing duration, climbing deterrence, and activity. Significant effects were observed within each parameter. D. variabilis showed the greatest general susceptibility to each repellent, followed by A. americanum, and I. scapularis. The most important and integrative measure of repellency was climbing deterrence–a measure of the spatial repellent’s ability to disrupt a tick’s natural propensity to climb. Transfluthrin deterred 75% of D. variabilis, 67% of A. americanum, and 50% of I. scapularis. Metofluthrin was slightly more effective, deterring 81% of D. variabilis, 73% of A. americanum, and 72% of I. scapularis. The present study poses a novel paradigm for repellency and reports a preliminary assessment of spatial repellent effect on tick behavior. Further research will assess spatial repellency in a more natural setting, scale exposure conditions, and incorporate host cues. 2023-06-23T13:50:36Z 2023-06-23T13:50:36Z 2022 Artículo de Publicación Periódica Siegel EL, Olivera M, Roig EM, Perry M, Li AY, D’hers S, et al. (2022) Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay. PLoS ONE 17(11): e0269150. https://doi. org/10.1371/journal.pone.0269150 https://ri.itba.edu.ar/handle/123456789/4192 en application/pdf Plos One
institution Instituto Tecnológico de Buenos Aires (ITBA)
institution_str I-32
repository_str R-138
collection Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA)
language Inglés
topic REPELENTES
INSECTOS
spellingShingle REPELENTES
INSECTOS
Siegel, Eric L.
Olivera, Marcos
Martínez Roig, Esteban
Perry, Melynda
Li, Andrew Y.
D'hers, Sebastián
Elman, Noel M.
Rich, Stephen M.
Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay
topic_facet REPELENTES
INSECTOS
description Repellents serve an important role in bite protection. Tick repellents largely rely on biomechanisms that induce responses with direct contact, but synthetic pyrethroids used as spatial repellents against insects have received recent attention for potential use in tick protection systems. An in vitro vertical climb assay was designed to assess spatial repellency against Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis adult, female ticks. Climbing behavior was assessed with and without the presence of two spatial repellents, transfluthrin and metofluthrin. Repellency parameters were defined to simulate the natural questing behavior of ambushing ticks, including measures of detachment, pseudo-questing duration, climbing deterrence, and activity. Significant effects were observed within each parameter. D. variabilis showed the greatest general susceptibility to each repellent, followed by A. americanum, and I. scapularis. The most important and integrative measure of repellency was climbing deterrence–a measure of the spatial repellent’s ability to disrupt a tick’s natural propensity to climb. Transfluthrin deterred 75% of D. variabilis, 67% of A. americanum, and 50% of I. scapularis. Metofluthrin was slightly more effective, deterring 81% of D. variabilis, 73% of A. americanum, and 72% of I. scapularis. The present study poses a novel paradigm for repellency and reports a preliminary assessment of spatial repellent effect on tick behavior. Further research will assess spatial repellency in a more natural setting, scale exposure conditions, and incorporate host cues.
format Artículo de Publicación Periódica
author Siegel, Eric L.
Olivera, Marcos
Martínez Roig, Esteban
Perry, Melynda
Li, Andrew Y.
D'hers, Sebastián
Elman, Noel M.
Rich, Stephen M.
author_facet Siegel, Eric L.
Olivera, Marcos
Martínez Roig, Esteban
Perry, Melynda
Li, Andrew Y.
D'hers, Sebastián
Elman, Noel M.
Rich, Stephen M.
author_sort Siegel, Eric L.
title Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay
title_short Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay
title_full Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay
title_fullStr Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay
title_full_unstemmed Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay
title_sort spatial repellents transfluthrin and metofluthrin affect the behavior of dermacentor variabilis, amblyomma americanum, and ixodes scapularis in an in vitro vertical climb assay
publisher Plos One
publishDate 2023
url https://ri.itba.edu.ar/handle/123456789/4192
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