Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches

Abstract: Erwin London dedicated considerable effort to understanding lipid interactions with membrane-resident proteins and how these interactions shaped the formation and maintenance of lipid phases and domains. In this endeavor, he developed ad hoc techniques that greatly contributed to advanceme...

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Autor principal: Barrantes, Francisco José
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2022
Materias:
RECEPTOR NICOTINICO
RECEPTOR DE ACETILCOLINA
FLUORESCENCIA
MEMBRANAS CELULARES
LIPIDOS
PROTEINAS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/14432
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-14432
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic RECEPTOR NICOTINICO
RECEPTOR DE ACETILCOLINA
FLUORESCENCIA
MEMBRANAS CELULARES
LIPIDOS
PROTEINAS
spellingShingle RECEPTOR NICOTINICO
RECEPTOR DE ACETILCOLINA
FLUORESCENCIA
MEMBRANAS CELULARES
LIPIDOS
PROTEINAS
Barrantes, Francisco José
Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches
topic_facet RECEPTOR NICOTINICO
RECEPTOR DE ACETILCOLINA
FLUORESCENCIA
MEMBRANAS CELULARES
LIPIDOS
PROTEINAS
description Abstract: Erwin London dedicated considerable effort to understanding lipid interactions with membrane-resident proteins and how these interactions shaped the formation and maintenance of lipid phases and domains. In this endeavor, he developed ad hoc techniques that greatly contributed to advancements in the field. We have employed and/or modified/extended some of his methodological approaches and applied them to investigate lipid interaction with the nicotinic acetylcholine receptor (nAChR) protein, the paradigm member of the superfamily of rapid pentameric ligand-gated ion channels (pLGIC). Our experimental systems ranged from purified receptor protein reconstituted into synthetic lipid membranes having known effects on receptor function, to cellular systems subjected to modification of their lipid content, e.g., varying cholesterol levels. We have often employed fluorescence techniques, including fluorescence quenching of diphenylhexatriene (DPH) extrinsic fluorescence and of nAChR intrinsic fluorescence by nitroxide spin-labeled phospholipids, DPH anisotropy, excimer formation of pyrene-phosphatidylcholine, and Förster resonance energy transfer (FRET) from the protein moiety to the extrinsic probes Laurdan, DPH, or pyrene-phospholipid to characterize various biophysical properties of lipid-receptor interactions. Some of these strategies are revisited in this review. Special attention is devoted to the anionic phospholipid phosphatidic acid (PA), which stabilizes the functional resting form of the nAChR. The receptor protein was shown to organize its PA-containing immediate microenvironment into microdomains with high lateral packing density and rigidity. PA and cholesterol appear to compete for the same binding sites on the nAChR protein.
format Artículo
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_sort Barrantes, Francisco José
title Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches
title_short Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches
title_full Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches
title_fullStr Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches
title_full_unstemmed Fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: The influence of Erwin London's methodological approaches
title_sort fluorescence studies of nicotinic acetylcholine receptor and its associated lipid milieu: the influence of erwin london's methodological approaches
publisher Springer
publishDate 2022
url https://repositorio.uca.edu.ar/handle/123456789/14432
work_keys_str_mv AT barrantesfranciscojose fluorescencestudiesofnicotinicacetylcholinereceptoranditsassociatedlipidmilieutheinfluenceoferwinlondonsmethodologicalapproaches
bdutipo_str Repositorios
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