The retinal basis of light aversion in neonatal mice
Aversive responses to bright light (photoaversion) require signaling from the eye to the brain. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) encode absolute light intensity and are thought to provide the light signals for photoaversion. Consistent with this, ne...
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Society for Neuroscience
2023
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/17413 |
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I33-R139-123456789-17413 |
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I33-R139-123456789-174132023-11-23T18:13:44Z The retinal basis of light aversion in neonatal mice Caval Holme, Franklin S. Aranda, Marcos L. Chen, Andy Q. Tiriac, Alexandre Zhang, Yizhen Smith, Benjamin Birnbaumer, Lutz Schmidt, Tiffany M. Feller, Marla B. RETINA DESARROLLO ENUCLEACION CORRIENTE FOTOELECTRICA FOTOFOBIA Aversive responses to bright light (photoaversion) require signaling from the eye to the brain. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) encode absolute light intensity and are thought to provide the light signals for photoaversion. Consistent with this, neonatal mice exhibit photoaversion before the developmental onset of image vision, and melanopsin deletion abolishes photoaversion in neonates. It is not well understood how the population of ipRGCs, which constitutes multiple physiologically distinct types (denoted M1-M6 in mouse), encodes light stimuli to produce an aversive response. Here, we provide several lines of evidence that M1 ipRGCs that lack the Brn3b transcription factor drive photoaversion in neonatal mice. First, neonatal mice lacking TRPC6 and TRPC7 ion channels failed to turn away from bright light, while two photon Ca21 imaging of their acutely isolated retinas revealed reduced photosensitivity in M1 ipRGCs, but not other ipRGC types. Second, mice in which all ipRGC types except for Brn3bnegative M1 ipRGCs are ablated exhibited normal photoaversion. Third, pharmacological blockade or genetic knockout of gap junction channels expressed by ipRGCs, which reduces the light sensitivity of M2-M6 ipRGCs in the neonatal retina, had small effects on photoaversion only at the brightest light intensities. Finally, M1s were not strongly depolarized by spontaneous retinal waves, a robust source of activity in the developing retina that depolarizes all other ipRGC types. M1s therefore constitute a separate information channel between the neonatal retina and brain that could ensure behavioral responses to light but not spontaneous retinal waves... 2023-11-06T23:14:45Z 2023-11-06T23:14:45Z 2022 Artículo Caval Holme, F. S. The retinal basis of light aversion in neonatal mice [en línea]. The Journal of Neuroscience. 2022, 42(20) 4101-4115. doi: 10.1523/JNEUROSCI.0151-22.2022. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/17413 0270-6474 (impreso) 1529-2401 (online) https://repositorio.uca.edu.ar/handle/123456789/17413 10.1523/JNEUROSCI.0151-22.2022 35396331 eng Acceso abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Society for Neuroscience The Journal of Neuroscience. Vol.42, No.20, 4101-4115, 2022 |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
RETINA DESARROLLO ENUCLEACION CORRIENTE FOTOELECTRICA FOTOFOBIA |
| spellingShingle |
RETINA DESARROLLO ENUCLEACION CORRIENTE FOTOELECTRICA FOTOFOBIA Caval Holme, Franklin S. Aranda, Marcos L. Chen, Andy Q. Tiriac, Alexandre Zhang, Yizhen Smith, Benjamin Birnbaumer, Lutz Schmidt, Tiffany M. Feller, Marla B. The retinal basis of light aversion in neonatal mice |
| topic_facet |
RETINA DESARROLLO ENUCLEACION CORRIENTE FOTOELECTRICA FOTOFOBIA |
| description |
Aversive responses to bright light (photoaversion) require signaling from the eye to the brain. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) encode absolute light intensity and are thought to provide the light signals for photoaversion.
Consistent with this, neonatal mice exhibit photoaversion before the developmental onset of image vision, and melanopsin deletion
abolishes photoaversion in neonates. It is not well understood how the population of ipRGCs, which constitutes multiple physiologically
distinct types (denoted M1-M6 in mouse), encodes light stimuli to produce an aversive response. Here, we provide several lines of evidence that M1 ipRGCs that lack the Brn3b transcription factor drive photoaversion in neonatal mice. First, neonatal mice lacking
TRPC6 and TRPC7 ion channels failed to turn away from bright light, while two photon Ca21 imaging of their acutely isolated retinas
revealed reduced photosensitivity in M1 ipRGCs, but not other ipRGC types. Second, mice in which all ipRGC types except for Brn3bnegative M1 ipRGCs are ablated exhibited normal photoaversion. Third, pharmacological blockade or genetic knockout of gap junction
channels expressed by ipRGCs, which reduces the light sensitivity of M2-M6 ipRGCs in the neonatal retina, had small effects on photoaversion only at the brightest light intensities. Finally, M1s were not strongly depolarized by spontaneous retinal waves, a robust source
of activity in the developing retina that depolarizes all other ipRGC types. M1s therefore constitute a separate information channel
between the neonatal retina and brain that could ensure behavioral responses to light but not spontaneous retinal waves... |
| format |
Artículo |
| author |
Caval Holme, Franklin S. Aranda, Marcos L. Chen, Andy Q. Tiriac, Alexandre Zhang, Yizhen Smith, Benjamin Birnbaumer, Lutz Schmidt, Tiffany M. Feller, Marla B. |
| author_facet |
Caval Holme, Franklin S. Aranda, Marcos L. Chen, Andy Q. Tiriac, Alexandre Zhang, Yizhen Smith, Benjamin Birnbaumer, Lutz Schmidt, Tiffany M. Feller, Marla B. |
| author_sort |
Caval Holme, Franklin S. |
| title |
The retinal basis of light aversion in neonatal mice |
| title_short |
The retinal basis of light aversion in neonatal mice |
| title_full |
The retinal basis of light aversion in neonatal mice |
| title_fullStr |
The retinal basis of light aversion in neonatal mice |
| title_full_unstemmed |
The retinal basis of light aversion in neonatal mice |
| title_sort |
retinal basis of light aversion in neonatal mice |
| publisher |
Society for Neuroscience |
| publishDate |
2023 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/17413 |
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1807949352009728000 |