Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development
The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population...
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| Formato: | Artículo |
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Elsevier
2025
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| Acceso en línea: | https://repositorio.uca.edu.ar/handle/123456789/19338 |
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I33-R139-123456789-193382025-02-11T05:02:08Z Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development Konrad, J. Clérico, G. Garrido, M. J. Taminelli, G. Yuponi, M. Yuponi, R. Crudeli, G. Sansinena, M. FERTILIZACION IN VITRO REPRODUCCION ASISTIDA REPRODUCCION ANIMAL OVOCITOS EMBRIONES BUFALO The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n = 6) or 14-day (n = 6) OPU interval groups. Although there was no significant difference in the average number of small (<3 mm) and large (>8 mm) diameter follicles available per OPU, a higher proportion of medium-sized follicles (3–8 mm) were observed in the 14-day interval group (5.129 vs 3.267; p < 0.05). The number of recovered oocytes per donor was also significantly higher (4.51 vs. 2.8; p < 0.05) in the 14-day interval group, although this was attributed to an increase in the proportion of lower quality oocytes (grades III and IV). After in vitro fertilization, embryo developmental competence from grade I and II oocytes was superior to that from grade III and IV oocytes, irrespective of OPU interval group. There was no significant difference in the proportion of grade I and II oocytes cleaved after sperm co-incubation; however, there was a higher proportion of blastocysts produced in 14-day interval group (28 vs. 6%, p < 0.05). No blastocysts were produced from grade III and IV oocytes. This study indicates it is possible to use a 14-day interval for oocyte collection in water buffalo; this approach could be considered as an alternative when access to animals is restricted. 2025-02-10T14:18:29Z 2025-02-10T14:18:29Z 2017 Artículo 0378-4320 https://repositorio.uca.edu.ar/handle/123456789/19338 10.1016/j.anireprosci.2017.06.004 28662882 eng Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Elsevier Animal Reproduction Science, 183. 2017. |
| institution |
Universidad Católica Argentina |
| institution_str |
I-33 |
| repository_str |
R-139 |
| collection |
Repositorio Institucional de la Universidad Católica Argentina (UCA) |
| language |
Inglés |
| topic |
FERTILIZACION IN VITRO REPRODUCCION ASISTIDA REPRODUCCION ANIMAL OVOCITOS EMBRIONES BUFALO |
| spellingShingle |
FERTILIZACION IN VITRO REPRODUCCION ASISTIDA REPRODUCCION ANIMAL OVOCITOS EMBRIONES BUFALO Konrad, J. Clérico, G. Garrido, M. J. Taminelli, G. Yuponi, M. Yuponi, R. Crudeli, G. Sansinena, M. Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development |
| topic_facet |
FERTILIZACION IN VITRO REPRODUCCION ASISTIDA REPRODUCCION ANIMAL OVOCITOS EMBRIONES BUFALO |
| description |
The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n = 6) or 14-day (n = 6) OPU interval groups. Although there was no significant difference in the average number of small (<3 mm) and large (>8 mm) diameter follicles available per OPU, a higher proportion of medium-sized follicles (3–8 mm) were observed in the 14-day interval group (5.129 vs 3.267; p < 0.05). The number of recovered oocytes per donor was also significantly higher (4.51 vs. 2.8; p < 0.05) in the 14-day interval group, although this was attributed to an increase in the proportion of lower quality oocytes (grades III and IV). After in vitro fertilization, embryo developmental competence from grade I and II oocytes was superior to that from grade III and IV oocytes, irrespective of OPU interval group. There was no significant difference in the proportion of grade I and II oocytes cleaved after sperm co-incubation; however, there was a higher proportion of blastocysts produced in 14-day interval group (28 vs. 6%, p < 0.05). No blastocysts were produced from grade III and IV oocytes. This study indicates it is possible to use a 14-day interval for oocyte collection in water buffalo; this approach could be considered as an alternative when access to animals is restricted. |
| format |
Artículo |
| author |
Konrad, J. Clérico, G. Garrido, M. J. Taminelli, G. Yuponi, M. Yuponi, R. Crudeli, G. Sansinena, M. |
| author_facet |
Konrad, J. Clérico, G. Garrido, M. J. Taminelli, G. Yuponi, M. Yuponi, R. Crudeli, G. Sansinena, M. |
| author_sort |
Konrad, J. |
| title |
Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development |
| title_short |
Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development |
| title_full |
Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development |
| title_fullStr |
Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development |
| title_full_unstemmed |
Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development |
| title_sort |
ovum pick-up interval in buffalo (bubalus bubalis) managed under wetland conditions in argentina: effect on follicular population, oocyte recovery, and in vitro embryo development |
| publisher |
Elsevier |
| publishDate |
2025 |
| url |
https://repositorio.uca.edu.ar/handle/123456789/19338 |
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