Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity

Fluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine in vitro fertilization (I...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Campagna, Anabella Andrea, Fabra, Mariana Carolina, Nikoloff, Noelia, Carranza Martin, Ana Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/20653
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-20653
record_format dspace
spelling I33-R139-123456789-206532025-09-30T11:09:55Z Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity Campagna, Anabella Andrea Fabra, Mariana Carolina Nikoloff, Noelia Carranza Martin, Ana Cristina FERTILIZACION IN VITRO ANTIPARASITARIOS TOXICIDAD Fluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine in vitro fertilization (IVF). Frozen-thawed sperm were exposed to fluazuron (50 µg/mL) for 2 h and evaluated for motility, mitochondrial activity, and membrane integrity, while BOECs were cultured with fluazuron for 24 h and assessed using MTT and comet assays. IVF was performed with gametes exposed to fluazuron for 18 h to evaluate pronuclear formation, and 24 h to study embryo development. Short-term fluazuron did not significantly affect sperm motility, viability, or membrane function. However, it decreased viability and induced DNA damage in BOECs after 24 h of treatment. Following fluazuron exposure during IVF, no differences were observed between the fluazuron-treated group and the control group in terms of pronuclear formation. However, a significantly higher incidence of abnormally condensed chromatin within the pronuclei was detected in the fluazuron-treated group. Furthermore, embryonic development decreased in both blastocyst and hatching rates in fluazuron treatment. These findings suggest that fluazuron may disrupt early reproductive events through multiple mechanisms, including BOEC dysfunction and chromatin damage. Our results highlight the importance of evaluating reproductive risks associated with antiparasitic drug exposure in livestock 2025-09-29T22:53:07Z 2025-09-29T22:53:07Z 2025 Artículo 1642-431X https://repositorio.uca.edu.ar/handle/123456789/20653 10.1016/j.repbio.2025.101087 eng application/pdf Elsevier Reproductive Biology. 25, 2025
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
spellingShingle FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
Campagna, Anabella Andrea
Fabra, Mariana Carolina
Nikoloff, Noelia
Carranza Martin, Ana Cristina
Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
topic_facet FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
description Fluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine in vitro fertilization (IVF). Frozen-thawed sperm were exposed to fluazuron (50 µg/mL) for 2 h and evaluated for motility, mitochondrial activity, and membrane integrity, while BOECs were cultured with fluazuron for 24 h and assessed using MTT and comet assays. IVF was performed with gametes exposed to fluazuron for 18 h to evaluate pronuclear formation, and 24 h to study embryo development. Short-term fluazuron did not significantly affect sperm motility, viability, or membrane function. However, it decreased viability and induced DNA damage in BOECs after 24 h of treatment. Following fluazuron exposure during IVF, no differences were observed between the fluazuron-treated group and the control group in terms of pronuclear formation. However, a significantly higher incidence of abnormally condensed chromatin within the pronuclei was detected in the fluazuron-treated group. Furthermore, embryonic development decreased in both blastocyst and hatching rates in fluazuron treatment. These findings suggest that fluazuron may disrupt early reproductive events through multiple mechanisms, including BOEC dysfunction and chromatin damage. Our results highlight the importance of evaluating reproductive risks associated with antiparasitic drug exposure in livestock
format Artículo
author Campagna, Anabella Andrea
Fabra, Mariana Carolina
Nikoloff, Noelia
Carranza Martin, Ana Cristina
author_facet Campagna, Anabella Andrea
Fabra, Mariana Carolina
Nikoloff, Noelia
Carranza Martin, Ana Cristina
author_sort Campagna, Anabella Andrea
title Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_short Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_full Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_fullStr Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_full_unstemmed Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_sort toxicological effects of fluazuron on bovine reproductive cells: implications for fertilization, sperm quality, and oviductal integrity
publisher Elsevier
publishDate 2025
url https://repositorio.uca.edu.ar/handle/123456789/20653
work_keys_str_mv AT campagnaanabellaandrea toxicologicaleffectsoffluazurononbovinereproductivecellsimplicationsforfertilizationspermqualityandoviductalintegrity
AT fabramarianacarolina toxicologicaleffectsoffluazurononbovinereproductivecellsimplicationsforfertilizationspermqualityandoviductalintegrity
AT nikoloffnoelia toxicologicaleffectsoffluazurononbovinereproductivecellsimplicationsforfertilizationspermqualityandoviductalintegrity
AT carranzamartinanacristina toxicologicaleffectsoffluazurononbovinereproductivecellsimplicationsforfertilizationspermqualityandoviductalintegrity
_version_ 1845298080439599104

Ejemplares similares