Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum

Grapevine (Vitis vinifera) is an economically crucial crop for agriculture, especially in Mediterranean regions. Xylella fastidiosa subsp. fastidiosa (Xff), a bacterium responsible for serious plant diseases as Pierce’s disease, poses a growing threat to viticulture. This study aims 1) to explore th...

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Autores principales: Perelló, A., Romero Munar, A., Martinez, I., Busquets, A., Cañellas, M., Quetglas, B. M., Bosch, R., Vadell, J., Cabot, C., Gomila, M.
Formato: Artículo
Lenguaje:Español
Publicado: MDPI 2025
Materias:
AGRICULTURA
CULTIVO ALIMENTICIO
VITICULTURA
MEJORA DE CULTIVOS
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/20826
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-20826
record_format dspace
spelling I33-R139-123456789-208262025-11-25T14:32:42Z Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum Perelló, A. Romero Munar, A. Martinez, I. Busquets, A. Cañellas, M. Quetglas, B. M. Bosch, R. Vadell, J. Cabot, C. Gomila, M. AGRICULTURA CULTIVO ALIMENTICIO VITICULTURA MEJORA DE CULTIVOS Grapevine (Vitis vinifera) is an economically crucial crop for agriculture, especially in Mediterranean regions. Xylella fastidiosa subsp. fastidiosa (Xff), a bacterium responsible for serious plant diseases as Pierce’s disease, poses a growing threat to viticulture. This study aims 1) to explore the diversity of culturable fungal endophytic communities present in the grapevine sap of naturally Xff-infected grapevines in the field and 2) to study the interaction between a pathogenic fungus identified in the xylem sap with Xff. Xylem sap was collected from vines of cv. Cabernet Sauvignon in a vineyard in Mallorca, Spain. The microbial communities were analyzed using traditional culture-depending techniques for fungal identification. Beneficial species identified included Aureobasidium pullulans and Rhodotorula mucilaginosa, which have antagonistic activities against fungal species associated with grapevine trunk diseases (GTDs). Nonetheless, the pathogenic fungi Sclerotinia sclerotiorum (Ss) and some members of the Dematiaceous taxonomic group like Phoma complex, Cladosporium sp, and Alternaria alternata were also identified. A similar diversity of fungal species was found in plants that tested negative and positive for the presence of Xff. Despite the small samples size used, these preliminary results suggest a potential complex interaction between Xff and the sap endophytic microbiome. In 28 addition, the interaction between Xff and Ss was further studied in grapewines artificially inoculated with Xff under controlled conditions. Interestingly, the results showed a synergistic effect, as Xff-inoculated grapevines were more vulnerable to Ss infection. This study provides novel insights into the fungal endophytic communities associated with Xff in grapevines-infected plants, and highlights some potential interactions among the bacteria and the sap microbiome components. During microbial interference, depending on the edaphoclimatic and crop managing conditions, while some of the identified mycobiota members could prove beneficial yielding plants more tolerant to Xff, others could be detrimental for grapevines as Xff could accelerate fungal diseases. 2025-11-06T20:10:55Z 2025-11-06T20:10:55Z 2025 Artículo 2223-7747 https://repositorio.uca.edu.ar/handle/123456789/20826 spa Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf España MDPI Plants. 2025, 14
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Español
topic AGRICULTURA
CULTIVO ALIMENTICIO
VITICULTURA
MEJORA DE CULTIVOS
spellingShingle AGRICULTURA
CULTIVO ALIMENTICIO
VITICULTURA
MEJORA DE CULTIVOS
Perelló, A.
Romero Munar, A.
Martinez, I.
Busquets, A.
Cañellas, M.
Quetglas, B. M.
Bosch, R.
Vadell, J.
Cabot, C.
Gomila, M.
Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum
topic_facet AGRICULTURA
CULTIVO ALIMENTICIO
VITICULTURA
MEJORA DE CULTIVOS
description Grapevine (Vitis vinifera) is an economically crucial crop for agriculture, especially in Mediterranean regions. Xylella fastidiosa subsp. fastidiosa (Xff), a bacterium responsible for serious plant diseases as Pierce’s disease, poses a growing threat to viticulture. This study aims 1) to explore the diversity of culturable fungal endophytic communities present in the grapevine sap of naturally Xff-infected grapevines in the field and 2) to study the interaction between a pathogenic fungus identified in the xylem sap with Xff. Xylem sap was collected from vines of cv. Cabernet Sauvignon in a vineyard in Mallorca, Spain. The microbial communities were analyzed using traditional culture-depending techniques for fungal identification. Beneficial species identified included Aureobasidium pullulans and Rhodotorula mucilaginosa, which have antagonistic activities against fungal species associated with grapevine trunk diseases (GTDs). Nonetheless, the pathogenic fungi Sclerotinia sclerotiorum (Ss) and some members of the Dematiaceous taxonomic group like Phoma complex, Cladosporium sp, and Alternaria alternata were also identified. A similar diversity of fungal species was found in plants that tested negative and positive for the presence of Xff. Despite the small samples size used, these preliminary results suggest a potential complex interaction between Xff and the sap endophytic microbiome. In 28 addition, the interaction between Xff and Ss was further studied in grapewines artificially inoculated with Xff under controlled conditions. Interestingly, the results showed a synergistic effect, as Xff-inoculated grapevines were more vulnerable to Ss infection. This study provides novel insights into the fungal endophytic communities associated with Xff in grapevines-infected plants, and highlights some potential interactions among the bacteria and the sap microbiome components. During microbial interference, depending on the edaphoclimatic and crop managing conditions, while some of the identified mycobiota members could prove beneficial yielding plants more tolerant to Xff, others could be detrimental for grapevines as Xff could accelerate fungal diseases.
format Artículo
author Perelló, A.
Romero Munar, A.
Martinez, I.
Busquets, A.
Cañellas, M.
Quetglas, B. M.
Bosch, R.
Vadell, J.
Cabot, C.
Gomila, M.
author_facet Perelló, A.
Romero Munar, A.
Martinez, I.
Busquets, A.
Cañellas, M.
Quetglas, B. M.
Bosch, R.
Vadell, J.
Cabot, C.
Gomila, M.
author_sort Perelló, A.
title Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum
title_short Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum
title_full Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum
title_fullStr Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum
title_full_unstemmed Xylem Sap Mycobiome in Grapevine Naturally Infected with 2 Xylella fastidiosa. A Case Study: Interaction of 3 Xylella fastidiosa with Sclerotinia sclerotiorum
title_sort xylem sap mycobiome in grapevine naturally infected with 2 xylella fastidiosa. a case study: interaction of 3 xylella fastidiosa with sclerotinia sclerotiorum
publisher MDPI
publishDate 2025
url https://repositorio.uca.edu.ar/handle/123456789/20826
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