Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors

This article presents an original and detailed study of semi continuous lab-scale anaerobic bioreactors subjected to Sulfate Loads Rates (SLRs) >130 mgSO4 2− /day.Lreactor. At these sulfate inputs, hydrogen sulfide yield by bacterial conversion can reach concentrations above 400 mg/L in the liqui...

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Autores principales: Girardi, Valentina, Tondo, María Laura, Balaban, Cecilia Lucía, Herrero, María Sol, Olivera, Camila, Salvatierra, Lucas Matías
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
BIOGAS
SULFATOS
SULFURO
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/20830
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Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139-123456789-20830
record_format dspace
spelling I33-R139-123456789-208302025-11-08T05:01:46Z Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors Girardi, Valentina Tondo, María Laura Balaban, Cecilia Lucía Herrero, María Sol Olivera, Camila Salvatierra, Lucas Matías BIOGAS SULFATOS SULFURO This article presents an original and detailed study of semi continuous lab-scale anaerobic bioreactors subjected to Sulfate Loads Rates (SLRs) >130 mgSO4 2− /day.Lreactor. At these sulfate inputs, hydrogen sulfide yield by bacterial conversion can reach concentrations above 400 mg/L in the liquid phase and more than 25,000 ppm in gas phase, respectively. Both values comprise significant technical challenges in biogas plants as well as health and environmental concerns. In order to control sulfide in liquid phase, two bioreactors were treated with opposite ferric dosing strategies: one underwent five consecutive cycles of shock precipitation of hydrogen sulfide, reducing peak levels from ~400 mg/L to <40 mg/L; the other involved the application of a more continuous and fine-tuned dosing strategy to manage sulfide concentrations across different stepwise levels: 400–300, 300–200, 200–100, and 100 to <10 mg/L. A third bioreactor, without iron addition, stabilized at ~250 mg/L H2S and 10,000 ppm in biogas over 140 days. A detailed sulfur mass balance enabled analysis of sulfate-to-sulfide conversion rates, Henry’s con stants between liquid and gas phases, and Fe3+/S2− molar ratios for both strategies. Values of 0.99 and 0.65 were obtained, matching with the stoichiometry of FeS during shock precipitation and FeS + S0 and/or Fe2S3 in stepwise precipitation, respectively. High-throughput sequencing of the 16S rRNA gene revealed subtle yet significant changes in the microbial community structures within bioreactors subjected to high sulfate loads and iron addition. Furthermore, the authors characterized the late-stage microbial response following methanogenic process inhibition and the cessation of biogas production. 2025-11-07T20:20:19Z 2025-11-07T20:20:19Z 2025 Artículo 2214-7144 https://repositorio.uca.edu.ar/handle/123456789/20830 10.1016/j.jwpe.2025.108405 eng application/pdf Elsevier Journal of Water Process Engineering. 2025 77
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic BIOGAS
SULFATOS
SULFURO
spellingShingle BIOGAS
SULFATOS
SULFURO
Girardi, Valentina
Tondo, María Laura
Balaban, Cecilia Lucía
Herrero, María Sol
Olivera, Camila
Salvatierra, Lucas Matías
Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
topic_facet BIOGAS
SULFATOS
SULFURO
description This article presents an original and detailed study of semi continuous lab-scale anaerobic bioreactors subjected to Sulfate Loads Rates (SLRs) >130 mgSO4 2− /day.Lreactor. At these sulfate inputs, hydrogen sulfide yield by bacterial conversion can reach concentrations above 400 mg/L in the liquid phase and more than 25,000 ppm in gas phase, respectively. Both values comprise significant technical challenges in biogas plants as well as health and environmental concerns. In order to control sulfide in liquid phase, two bioreactors were treated with opposite ferric dosing strategies: one underwent five consecutive cycles of shock precipitation of hydrogen sulfide, reducing peak levels from ~400 mg/L to <40 mg/L; the other involved the application of a more continuous and fine-tuned dosing strategy to manage sulfide concentrations across different stepwise levels: 400–300, 300–200, 200–100, and 100 to <10 mg/L. A third bioreactor, without iron addition, stabilized at ~250 mg/L H2S and 10,000 ppm in biogas over 140 days. A detailed sulfur mass balance enabled analysis of sulfate-to-sulfide conversion rates, Henry’s con stants between liquid and gas phases, and Fe3+/S2− molar ratios for both strategies. Values of 0.99 and 0.65 were obtained, matching with the stoichiometry of FeS during shock precipitation and FeS + S0 and/or Fe2S3 in stepwise precipitation, respectively. High-throughput sequencing of the 16S rRNA gene revealed subtle yet significant changes in the microbial community structures within bioreactors subjected to high sulfate loads and iron addition. Furthermore, the authors characterized the late-stage microbial response following methanogenic process inhibition and the cessation of biogas production.
format Artículo
author Girardi, Valentina
Tondo, María Laura
Balaban, Cecilia Lucía
Herrero, María Sol
Olivera, Camila
Salvatierra, Lucas Matías
author_facet Girardi, Valentina
Tondo, María Laura
Balaban, Cecilia Lucía
Herrero, María Sol
Olivera, Camila
Salvatierra, Lucas Matías
author_sort Girardi, Valentina
title Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_short Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_full Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_fullStr Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_full_unstemmed Ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: A mass balance assessment and microbial structure implications in anaerobic bioreactors
title_sort ultra high sulfate loads and iron precision dosing for hydrogen sulfide management: a mass balance assessment and microbial structure implications in anaerobic bioreactors
publisher Elsevier
publishDate 2025
url https://repositorio.uca.edu.ar/handle/123456789/20830
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