24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment

Abstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received m...

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Autores principales: Jiménez Ortega, Vanesa, Cano Barquilla, Pilar, Cardinali, Daniel Pedro, Esquifino, Ana I.
Formato: Artículo
Lenguaje:Inglés
Inglés
Publicado: Taylor & Francis 2019
Materias:
MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/1626
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-1626
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
Inglés
topic MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
spellingShingle MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
topic_facet MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
description Abstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received melatonin (ca. 60 μg/day) in the drinking water. After 1 month, rats were killed at six different time intervals, throughout a 24-h cycle. MBH mRNA levels were measured by real-time PCR analysis. In controls NOS-1 mRNA expression did not exhibit 24-h variations while NOS-2 gene expression peaked at the early light phase of cycle. MBH mRNA for both the inducible (HO-1) and constitutive (HO-2) isoforms of HO showed a significant 24 h pattern with a maximum at the middle of the dark phase or at the early light phase, respectively. None of mRNAs encoding Cu/Zn-SOD, Mn-SOD and catalase exhibited significant 24-h variations in rat MBH under control conditions. Chronic melatonin administration in the drinking water decreased significantly mRNAs for NOS-1, NOS-2, HO-1 and HO-2 as well as changed their 24-h profile. Melatonin augmented gene expression of the antioxidant enzymes Cu/Zn-SOD, Mn-SOD or catalase at certain time intervals only
format Artículo
author Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_facet Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_sort Jiménez Ortega, Vanesa
title 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_short 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_full 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_fullStr 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_full_unstemmed 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_sort 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
publisher Taylor & Francis
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/1626
work_keys_str_mv AT jimenezortegavanesa 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment
AT canobarquillapilar 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment
AT cardinalidanielpedro 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment
AT esquifinoanai 24hourrhythmingeneexpressionofredoxpathwayenzymesinrathypothalamuseffectofmelatonintreatment
bdutipo_str Repositorios
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