TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice

Abstract: Excess production of reactive oxygen species (ROS) caused by hyperglycemia is a major risk factor for heart failure. We previously reported that transient receptor potential canonical 3 (TRPC3) channel mediates pressure overload-induced maladaptive cardiac fibrosis by forming stably functi...

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Autores principales: Oda, Sayaka, Numaga-Tomita, Takuro, Kitajima, Naoyuki, Toyama, Takashi, Harada, Eri, Shimauchi, Tsukasa, Nishimura, Akiyuki, Ishikawa, Tatsuya, Kumagai, Yoshito, Birnbaumer, Lutz, Nishida, Motohiro
Formato: Artículo
Lenguaje:Inglés
Publicado: Nature Research 2019
Materias:
HIPERGLUCEMIA
FIBROSIS
CORAZON
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/8722
Aporte de:
Repositorio Institucional de la Universidad Católica Argentina (UCA) de Universidad Católica Argentina
id I33-R139123456789-8722
record_format dspace
institution Universidad Católica Argentina
institution_str I-33
repository_str R-139
collection Repositorio Institucional de la Universidad Católica Argentina (UCA)
language Inglés
topic HIPERGLUCEMIA
FIBROSIS
CORAZON
spellingShingle HIPERGLUCEMIA
FIBROSIS
CORAZON
Oda, Sayaka
Numaga-Tomita, Takuro
Kitajima, Naoyuki
Toyama, Takashi
Harada, Eri
Shimauchi, Tsukasa
Nishimura, Akiyuki
Ishikawa, Tatsuya
Kumagai, Yoshito
Birnbaumer, Lutz
Nishida, Motohiro
TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
topic_facet HIPERGLUCEMIA
FIBROSIS
CORAZON
description Abstract: Excess production of reactive oxygen species (ROS) caused by hyperglycemia is a major risk factor for heart failure. We previously reported that transient receptor potential canonical 3 (TRPC3) channel mediates pressure overload-induced maladaptive cardiac fibrosis by forming stably functional complex with NADPH oxidase 2 (Nox2). Although TRPC3 has been long suggested to form hetero-multimer channels with TRPC6 and function as diacylglycerol-activated cation channels coordinately, the role of TRPC6 in heart is still obscure. We here demonstrated that deletion of TRPC6 had no impact on pressure overload-induced heart failure despite inhibiting interstitial fibrosis in mice. TRPC6-deficient mouse hearts 1 week after transverse aortic constriction showed comparable increases in fibrotic gene expressions and ROS production but promoted inductions of inflammatory cytokines, compared to wild type hearts. Treatment of TRPC6-deficient mice with streptozotocin caused severe reduction of cardiac contractility with enhancing urinary and cardiac lipid peroxide levels, compared to wild type and TRPC3-deficient mice. Knockdown of TRPC6, but not TRPC3, enhanced basal expression levels of cytokines in rat cardiomyocytes. TRPC6 could interact with Nox2, but the abundance of TRPC6 was inversely correlated with that of Nox2. These results strongly suggest that Nox2 destabilization through disrupting TRPC3-Nox2 complex underlies attenuation of hyperglycemia-induced heart failure by TRPC6.
format Artículo
author Oda, Sayaka
Numaga-Tomita, Takuro
Kitajima, Naoyuki
Toyama, Takashi
Harada, Eri
Shimauchi, Tsukasa
Nishimura, Akiyuki
Ishikawa, Tatsuya
Kumagai, Yoshito
Birnbaumer, Lutz
Nishida, Motohiro
author_facet Oda, Sayaka
Numaga-Tomita, Takuro
Kitajima, Naoyuki
Toyama, Takashi
Harada, Eri
Shimauchi, Tsukasa
Nishimura, Akiyuki
Ishikawa, Tatsuya
Kumagai, Yoshito
Birnbaumer, Lutz
Nishida, Motohiro
author_sort Oda, Sayaka
title TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
title_short TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
title_full TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
title_fullStr TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
title_full_unstemmed TRPC6 counteracts TRPC3-Nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
title_sort trpc6 counteracts trpc3-nox2 protein complex leading to attenuation of hyperglycemia-induced heart failure in mice
publisher Nature Research
publishDate 2019
url https://repositorio.uca.edu.ar/handle/123456789/8722
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