ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]

Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, t...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Nicolas, Juan Carlos, Grumelli, Yanina Alejandra
Formato: Artículo
Lenguaje:Español
Publicado: Federación Bioquímica de la Provincia de Buenos Aires 2004
Materias:
QD Química
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3982
record_format dspace
spelling I38-R144-39822025-04-11T18:57:14Z http://pa.bibdigital.ucc.edu.ar/3982/ ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón] Nicolas, Juan Carlos Grumelli, Yanina Alejandra QD Química Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples. Federación Bioquímica de la Provincia de Buenos Aires 2004-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf Nicolas, Juan Carlos ORCID: https://orcid.org/0000-0001-9163-2668 <https://orcid.org/0000-0001-9163-2668> and Grumelli, Yanina Alejandra ORCID: https://orcid.org/0000-0001-6234-865X <https://orcid.org/0000-0001-6234-865X> (2004) ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]. Acta Bioquímica Clínica Latinoamericana, 38 (1). pp. 23-27. ISSN 0325-2957
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
spellingShingle QD Química
Nicolas, Juan Carlos
Grumelli, Yanina Alejandra
ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
topic_facet QD Química
description Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples.
format Artículo
author Nicolas, Juan Carlos
Grumelli, Yanina Alejandra
author_facet Nicolas, Juan Carlos
Grumelli, Yanina Alejandra
author_sort Nicolas, Juan Carlos
title ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
title_short ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
title_full ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
title_fullStr ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
title_full_unstemmed ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]
title_sort elisa method for assess liver - kidney microsomes antibodies [método elisa para anticuerpos antimicrosomales hígado-riñón]
publisher Federación Bioquímica de la Provincia de Buenos Aires
publishDate 2004
url http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf
work_keys_str_mv AT nicolasjuancarlos elisamethodforassessliverkidneymicrosomesantibodiesmetodoelisaparaanticuerposantimicrosomaleshigadorinon
AT grumelliyaninaalejandra elisamethodforassessliverkidneymicrosomesantibodiesmetodoelisaparaanticuerposantimicrosomaleshigadorinon
_version_ 1832592419054419968

Ejemplares similares