Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae

Pyruvate decarboxylase (PDC) catalyzes the thiamine pyrophosphate-and magnesium-dependent decarboxylation of pyruvate to acetaldehyde and carbon dioxide, a relevant pathway in ethanol production. The budding yeast S. cerevisiae is a major player in alcoholic fermentation and a detailed knowledge of...

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Autor principal: Rosa, Alberto Luis dir.
Formato: proyecto_de_investigacion
Lenguaje:Español
Publicado: 2016
Materias:
QD Química
QH426 Genética
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/967/1/PI_Rosa.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-967
record_format dspace
spelling I38-R144-9672025-03-20T18:31:55Z http://pa.bibdigital.ucc.edu.ar/967/ Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae Rosa, Alberto Luis dir. QD Química QH426 Genética Pyruvate decarboxylase (PDC) catalyzes the thiamine pyrophosphate-and magnesium-dependent decarboxylation of pyruvate to acetaldehyde and carbon dioxide, a relevant pathway in ethanol production. The budding yeast S. cerevisiae is a major player in alcoholic fermentation and a detailed knowledge of the biochemistry and metabolic control of the fermentative pathway in this microorganism is available. Our laboratory has contributed to the study of the genes encoding PDC in the filamentous fungus N. crassa (i.e. cfp-1 and cfp-2). We also discovered that PDC in filamentous fungi assembles into macromolecular filaments co-localizing with cytoplasmic microtubules. We showed that abundance and organization of PDC-filaments in fungi is dependent on the carbon sources and/or metabolic conditions of the cell. In S. cerevisiae, three structural genes for PDC have been identified (i.e. PDC1, PDC5 and PDC6) but the potential genetic and protein diversity of PDC genes in indigenous strains of S. cerevisiae has not been systematically studied. Also, the subcellular distribution and potential supramolecular arrangement of PDC from indigenous S. crerevisiae strains, perhaps during budding growth on alternative carbon sources and/or pseudohyphal growth under nutrient deprivation, has not been explored yet. In this project we will explore the molecular genetic diversity, biochemical properties and subcellular distribution of wild PDC variants from indigenous S. cerevisiae strains. Results from this project will contribute to the knowledge of the genetic and phenotypic diversity of S. cerevisiae PDC genes and their protein products. These studies also constitute the basis for future analyses of PDC genes from the isolated indigenous non-Saccharomyces yeasts strains. 2016-03-01 proyecto_de_investigacion info:eu-repo/semantics/closedAccess application/pdf spa http://pa.bibdigital.ucc.edu.ar/967/1/PI_Rosa.pdf Rosa, Alberto Luis dir. ORCID: https://orcid.org/0000-0001-8701-4525 <https://orcid.org/0000-0001-8701-4525> (2016) Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae. [Proyecto de investigación]
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
QH426 Genética
spellingShingle QD Química
QH426 Genética
Rosa, Alberto Luis dir.
Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
topic_facet QD Química
QH426 Genética
description Pyruvate decarboxylase (PDC) catalyzes the thiamine pyrophosphate-and magnesium-dependent decarboxylation of pyruvate to acetaldehyde and carbon dioxide, a relevant pathway in ethanol production. The budding yeast S. cerevisiae is a major player in alcoholic fermentation and a detailed knowledge of the biochemistry and metabolic control of the fermentative pathway in this microorganism is available. Our laboratory has contributed to the study of the genes encoding PDC in the filamentous fungus N. crassa (i.e. cfp-1 and cfp-2). We also discovered that PDC in filamentous fungi assembles into macromolecular filaments co-localizing with cytoplasmic microtubules. We showed that abundance and organization of PDC-filaments in fungi is dependent on the carbon sources and/or metabolic conditions of the cell. In S. cerevisiae, three structural genes for PDC have been identified (i.e. PDC1, PDC5 and PDC6) but the potential genetic and protein diversity of PDC genes in indigenous strains of S. cerevisiae has not been systematically studied. Also, the subcellular distribution and potential supramolecular arrangement of PDC from indigenous S. crerevisiae strains, perhaps during budding growth on alternative carbon sources and/or pseudohyphal growth under nutrient deprivation, has not been explored yet. In this project we will explore the molecular genetic diversity, biochemical properties and subcellular distribution of wild PDC variants from indigenous S. cerevisiae strains. Results from this project will contribute to the knowledge of the genetic and phenotypic diversity of S. cerevisiae PDC genes and their protein products. These studies also constitute the basis for future analyses of PDC genes from the isolated indigenous non-Saccharomyces yeasts strains.
format proyecto_de_investigacion
author Rosa, Alberto Luis dir.
author_facet Rosa, Alberto Luis dir.
author_sort Rosa, Alberto Luis dir.
title Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
title_short Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
title_full Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
title_fullStr Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
title_full_unstemmed Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
title_sort biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae
publishDate 2016
url http://pa.bibdigital.ucc.edu.ar/967/1/PI_Rosa.pdf
work_keys_str_mv AT rosaalbertoluisdir biodiversidadgeneticaybioquimicadepiruvatodecarboxilasasencepasindigenasdesaccharomycescerevisiae
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