Detection of homologous resistance genes to the late blight in wild potatoes

The identification of resistance genes to late blight (Phytophthora infestans) is essential in potato (Solanum tuberosum) breeding programs to advance in obtaining resistant cultivars. The objective was to detect sequences homologous to the resistance genes (R1, R2, R3a, R8, Sto-448, Rpi-blb1, Rpi-b...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Enciso-Maldonado, Guillermo, Lozoya-Saldaña, Héctor, Talavera-Stefani, Liliana, Burgos-Cantoni, Cinthia, Mongelos-Franco, Yerutí
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Instituto de Botánica del Nordeste 2024
Materias:
Molecular markers
Phytophthora infestans
plant genetic resources
resistance genes
Genes de resistencia
marcadores moleculares
recursos fitogenéticos
Acceso en línea:https://revistas.unne.edu.ar/index.php/bon/article/view/6434
Aporte de:
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) de Universidad Nacional del Nordeste
id I48-R154-article-6434
record_format ojs
spelling I48-R154-article-64342024-10-17T20:10:58Z Detection of homologous resistance genes to the late blight in wild potatoes Detección de genes homólogos de resistencia al tizón tardío en patatas silvestres Enciso-Maldonado, Guillermo Lozoya-Saldaña, Héctor Talavera-Stefani, Liliana Burgos-Cantoni, Cinthia Mongelos-Franco, Yerutí Molecular markers Phytophthora infestans plant genetic resources resistance genes Genes de resistencia marcadores moleculares Phytophthora infestans recursos fitogenéticos The identification of resistance genes to late blight (Phytophthora infestans) is essential in potato (Solanum tuberosum) breeding programs to advance in obtaining resistant cultivars. The objective was to detect sequences homologous to the resistance genes (R1, R2, R3a, R8, Sto-448, Rpi-blb1, Rpi-blb2, and Rpi-ber1) to P. infestans in 23 accessions of 14 wild potato species (Solanum spp.) with different resistance levels through the amplification of molecular markers. Eight primers sets were used to amplify the molecular markers. No amplifications of resistance markers were observed in highly susceptible plants in the field (accession 631200 of S. chacoense, accession 653801 of S. berthaultii and accession 631201 of S. juzepzuckii), whereas in plants immune to the field, such as S. demissum (653770), amplification of six molecular markers was observed. The R3a marker did not amplify for any accession. Therefore, no apparent association between the number of markers amplified and the resistance category was found by Chi-square test. These results highlight the complexity of genetic resistance in the studied potato species. Future studies could evaluate the functionality of the homologous genes and detect other molecular markers of the resistance genes. La identificación de genes de resistencia al tizón tardío (Phytophthora infestans) es fundamental en programas de mejoramiento de papa (Solanum tuberosum) para avanzar en la obtención de cultivares resistentes. El objetivo fue detectar secuencias homólogas a los genes de resistencia (R1, R2, R3a, R8, Sto-448, Rpi-blb1, Rpi-blb2 y Rpi-ber1) a P. infestans en 23 accesiones de 14 especies silvestres de papa (Solanum spp.) con diferentes niveles de resistencia mediante la amplificación de marcadores moleculares. En plantas altamente susceptibles en campo no se observaron amplificaciones de marcadores de resistencia (accesión 631200 de S. chacoense, accesión 653801 de S. berthaultii y accesión 631201 de S. juzepzuckii), mientras que, en plantas inmunes a campo como S. demissum (653770), se observó amplificación de seis marcadores moleculares. El marcador R3a no amplificó para ninguna accesión. Mediante análisis de Chicuadrado no se encontró asociación aparente entre el número de marcadores amplificados y la categoría de resistencia. Estos resultados resaltan la complejidad de la resistencia genética en las especies de papa estudiadas. Futuros estudios podrían evaluar la funcionalidad de los genes homólogos y detectar otros marcadores moleculares para genes de resistencia. Instituto de Botánica del Nordeste 2024-08-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/bon/article/view/6434 10.30972/bon.3326434 Bonplandia; Vol. 33 No. 2 (2024); 271-276 Bonplandia; Vol. 33 Núm. 2 (2024); 271-276 1853-8460 0524-0476 eng https://revistas.unne.edu.ar/index.php/bon/article/view/6434/7227 Derechos de autor 2024 Bonplandia http://creativecommons.org/licenses/by/4.0
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-154
container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Inglés
format Artículo revista
topic Molecular markers
Phytophthora infestans
plant genetic resources
resistance genes
Genes de resistencia
marcadores moleculares
Phytophthora infestans
recursos fitogenéticos
spellingShingle Molecular markers
Phytophthora infestans
plant genetic resources
resistance genes
Genes de resistencia
marcadores moleculares
Phytophthora infestans
recursos fitogenéticos
Enciso-Maldonado, Guillermo
Lozoya-Saldaña, Héctor
Talavera-Stefani, Liliana
Burgos-Cantoni, Cinthia
Mongelos-Franco, Yerutí
Detection of homologous resistance genes to the late blight in wild potatoes
topic_facet Molecular markers
Phytophthora infestans
plant genetic resources
resistance genes
Genes de resistencia
marcadores moleculares
Phytophthora infestans
recursos fitogenéticos
author Enciso-Maldonado, Guillermo
Lozoya-Saldaña, Héctor
Talavera-Stefani, Liliana
Burgos-Cantoni, Cinthia
Mongelos-Franco, Yerutí
author_facet Enciso-Maldonado, Guillermo
Lozoya-Saldaña, Héctor
Talavera-Stefani, Liliana
Burgos-Cantoni, Cinthia
Mongelos-Franco, Yerutí
author_sort Enciso-Maldonado, Guillermo
title Detection of homologous resistance genes to the late blight in wild potatoes
title_short Detection of homologous resistance genes to the late blight in wild potatoes
title_full Detection of homologous resistance genes to the late blight in wild potatoes
title_fullStr Detection of homologous resistance genes to the late blight in wild potatoes
title_full_unstemmed Detection of homologous resistance genes to the late blight in wild potatoes
title_sort detection of homologous resistance genes to the late blight in wild potatoes
description The identification of resistance genes to late blight (Phytophthora infestans) is essential in potato (Solanum tuberosum) breeding programs to advance in obtaining resistant cultivars. The objective was to detect sequences homologous to the resistance genes (R1, R2, R3a, R8, Sto-448, Rpi-blb1, Rpi-blb2, and Rpi-ber1) to P. infestans in 23 accessions of 14 wild potato species (Solanum spp.) with different resistance levels through the amplification of molecular markers. Eight primers sets were used to amplify the molecular markers. No amplifications of resistance markers were observed in highly susceptible plants in the field (accession 631200 of S. chacoense, accession 653801 of S. berthaultii and accession 631201 of S. juzepzuckii), whereas in plants immune to the field, such as S. demissum (653770), amplification of six molecular markers was observed. The R3a marker did not amplify for any accession. Therefore, no apparent association between the number of markers amplified and the resistance category was found by Chi-square test. These results highlight the complexity of genetic resistance in the studied potato species. Future studies could evaluate the functionality of the homologous genes and detect other molecular markers of the resistance genes.
publisher Instituto de Botánica del Nordeste
publishDate 2024
url https://revistas.unne.edu.ar/index.php/bon/article/view/6434
work_keys_str_mv AT encisomaldonadoguillermo detectionofhomologousresistancegenestothelateblightinwildpotatoes
AT lozoyasaldanahector detectionofhomologousresistancegenestothelateblightinwildpotatoes
AT talaverastefanililiana detectionofhomologousresistancegenestothelateblightinwildpotatoes
AT burgoscantonicinthia detectionofhomologousresistancegenestothelateblightinwildpotatoes
AT mongelosfrancoyeruti detectionofhomologousresistancegenestothelateblightinwildpotatoes
AT encisomaldonadoguillermo detecciondegeneshomologosderesistenciaaltizontardioenpatatassilvestres
AT lozoyasaldanahector detecciondegeneshomologosderesistenciaaltizontardioenpatatassilvestres
AT talaverastefanililiana detecciondegeneshomologosderesistenciaaltizontardioenpatatassilvestres
AT burgoscantonicinthia detecciondegeneshomologosderesistenciaaltizontardioenpatatassilvestres
AT mongelosfrancoyeruti detecciondegeneshomologosderesistenciaaltizontardioenpatatassilvestres
first_indexed 2024-08-21T22:36:48Z
last_indexed 2025-05-17T05:11:20Z
_version_ 1832343233529643008

Ejemplares similares