Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows

The objective of this study was to evaluate the effect of two doses of equine chorionic gonadotropin (eCG) in a J-Synch protocol, using 107 cows with calves at foot, Holstein, Brown Swiss, and their crosses, with a body condition (CC) of 2.5 (scale 1-5), and a silvopastoral system. The trial consist...

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Autores principales: Vargas Fernandez, Tito Laban, Yánez Avalos, Darwin Omar, Ortega Coello, Manuel Edelberto, Culcay Troncoso, Israel H, Condo Plaza, Luis Alfonso, Marini, Pablo R.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2023
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Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/7044
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record_format ojs
institution Universidad Nacional del Nordeste
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container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Time of IATF
presence of estrus
GnRH
dual purpose
Hora de IATF
presencia de estro
GnRH
doble propósito
spellingShingle Time of IATF
presence of estrus
GnRH
dual purpose
Hora de IATF
presencia de estro
GnRH
doble propósito
Vargas Fernandez, Tito Laban
Yánez Avalos, Darwin Omar
Ortega Coello, Manuel Edelberto
Culcay Troncoso, Israel H
Condo Plaza, Luis Alfonso
Marini, Pablo R.
Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows
topic_facet Time of IATF
presence of estrus
GnRH
dual purpose
Hora de IATF
presencia de estro
GnRH
doble propósito
author Vargas Fernandez, Tito Laban
Yánez Avalos, Darwin Omar
Ortega Coello, Manuel Edelberto
Culcay Troncoso, Israel H
Condo Plaza, Luis Alfonso
Marini, Pablo R.
author_facet Vargas Fernandez, Tito Laban
Yánez Avalos, Darwin Omar
Ortega Coello, Manuel Edelberto
Culcay Troncoso, Israel H
Condo Plaza, Luis Alfonso
Marini, Pablo R.
author_sort Vargas Fernandez, Tito Laban
title Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows
title_short Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows
title_full Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows
title_fullStr Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows
title_full_unstemmed Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows
title_sort effect of ecg post protocol j - synch on pregnancy rate in ecuadorian amazon cows
description The objective of this study was to evaluate the effect of two doses of equine chorionic gonadotropin (eCG) in a J-Synch protocol, using 107 cows with calves at foot, Holstein, Brown Swiss, and their crosses, with a body condition (CC) of 2.5 (scale 1-5), and a silvopastoral system. The trial consisted of two treatments (T): T1 = 1 dose of eCG (n=52) and T2 = 2 doses of eCG (n=55). On day 0, an intravaginal device (BID) containing 0.5 g progesterone (P4) and 2 mg estradiol benzoate (BE) was administered. On day 6, the DIB was removed and 500 μg Cloprostenol (PGF2α) + 400 IU of eCG was administered. The base of the tail was painted, to determine estrus manifestation. Unpainted animals were inseminated at 60 h and painted animals at 72 h, + 100 μg of (GnRH). At T1 treatment, the size of the corpus luteum (CL) was measured on days 14 and 30 after insemination, and blood samples were taken. At T2 treatment, a second dose of 400 IU of eCG was administered on day 14, and CL size was measured, as well as on day 30, and blood samples were taken. The pregnancy diagnosis was made 40 days after the IATF, by ultrasonography. The highest pregnancy rate was observed in T2 (58.2%), and in cows in estrus (56.6%). No statistical differences were observed between treatments (p=0.7). Serum P4 concentrations were not different. Similarly, the diameter of the CL at days 14 and 30 post IATF did not differ (p>0.05). However, the size of the CL at days 14 and 30, is clearly related (p<0.001). In conclusion, a second dose of eCG on day 14 in the J - Synch protocol
publisher Universidad Nacional del Nordeste
publishDate 2023
url https://revistas.unne.edu.ar/index.php/vet/article/view/7044
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spelling I48-R154-article-70442023-11-28T12:58:58Z Effect of eCG post protocol J - Synch on pregnancy rate in Ecuadorian Amazon cows Efecto de la eCG pos protocolo J-Synch sobre la tasa de preñez en vacas de la Amazonía Ecuatoriana Vargas Fernandez, Tito Laban Yánez Avalos, Darwin Omar Ortega Coello, Manuel Edelberto Culcay Troncoso, Israel H Condo Plaza, Luis Alfonso Marini, Pablo R. Time of IATF presence of estrus GnRH dual purpose Hora de IATF presencia de estro GnRH doble propósito The objective of this study was to evaluate the effect of two doses of equine chorionic gonadotropin (eCG) in a J-Synch protocol, using 107 cows with calves at foot, Holstein, Brown Swiss, and their crosses, with a body condition (CC) of 2.5 (scale 1-5), and a silvopastoral system. The trial consisted of two treatments (T): T1 = 1 dose of eCG (n=52) and T2 = 2 doses of eCG (n=55). On day 0, an intravaginal device (BID) containing 0.5 g progesterone (P4) and 2 mg estradiol benzoate (BE) was administered. On day 6, the DIB was removed and 500 μg Cloprostenol (PGF2α) + 400 IU of eCG was administered. The base of the tail was painted, to determine estrus manifestation. Unpainted animals were inseminated at 60 h and painted animals at 72 h, + 100 μg of (GnRH). At T1 treatment, the size of the corpus luteum (CL) was measured on days 14 and 30 after insemination, and blood samples were taken. At T2 treatment, a second dose of 400 IU of eCG was administered on day 14, and CL size was measured, as well as on day 30, and blood samples were taken. The pregnancy diagnosis was made 40 days after the IATF, by ultrasonography. The highest pregnancy rate was observed in T2 (58.2%), and in cows in estrus (56.6%). No statistical differences were observed between treatments (p=0.7). Serum P4 concentrations were not different. Similarly, the diameter of the CL at days 14 and 30 post IATF did not differ (p>0.05). However, the size of the CL at days 14 and 30, is clearly related (p<0.001). In conclusion, a second dose of eCG on day 14 in the J - Synch protocol El objetivo de este estudio fue evaluar el efecto de dos dosis de gonadotropina coriónica equina (eCG) en el protocolo J-Synch, utilizando 107 vacas con cría al pie, de razas Holstein, Pardo Suizo y sus cruzas, con una condición corporal (CC) de 2,5 (escala 1-5), y un sistema silvo-pastoril. El estudio consistió de dos tratamientos (T): T1 = 1 dosis de eCG (n=52), y T2 = 2 dosis de eCG (n=55). El Día 0 se aplicó un dispositivo intravaginal (DIB) con 0,5 g de progesterona (P4) y 2 mg de benzoato de estradiol (BE). Al día 6, se retiró el DIB y se administraron 500 μg de Cloprostenol (PGF2α) + 400 UI de eCG. Se pintó la base de la cola, para determinar manifestación de estro, inseminando animales despintados a las 60 horas y pintados a las 72 horas, + 100 μg de (GnRH). En el T1 se midió el tamaño de cuerpo lúteo (CL) en los días 14 y 30 después de la inseminación y se tomaron muestras de sangre. En el T2, se administró una segunda dosis de 400 UI de (eCG) al día 14, y se midió el tamaño de CL, al igual en día 30, y se tomaron muestras de sangre. El diagnóstico de gestación se realizó a los 40 días después de la IATF, mediante ultrasonografía. La mayor tasa de preñez fue en el T2 (58,2%), y en vacas en estro (56,6%), sin evidenciar diferencias relevantes (p=0,7). Las concentraciones séricas de P4, no difieren. Asimismo, el diámetro del CL de los días 14 y 30 post IATF (p>0,05). Sin embargo, la magnitud del CL en los días 14 y 30, están claramente relacionados (p<0,001). En conclusión, una segunda dosis de eCG al día 14 en el protocolo J-Synch, no tuvo influencia sobre la preñez, pero si una tendencia a desempeñarse mejor en vacas de la Amazonía Ecuatoriana. Universidad Nacional del Nordeste 2023-11-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/7044 10.30972/vet.3427044 Revista Veterinaria; Vol. 34 Núm. 2 (2023); 55-60 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/7044/6500 https://creativecommons.org/licenses/by/2.5/ar/