A new method for animal preservation avoids the use of formaldehyde and improves preservation features

Dr. W. Thiel developed a human cadaver preservation technique that maintains essential featuressuch as tissue flexibility, elasticity, volume, and color, using minimal amounts of formaldehyde. This methodenables clear anatomical recognition without significant alterations in the preserved material....

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Autores principales: Pereyra, C. F., Parola, D. G., Biancardi, M. E., Pérez Mogetta, L. C., Cirimele, M. N., Venegas, V. L.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Nordeste 2025
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Acceso en línea:https://revistas.unne.edu.ar/index.php/vet/article/view/8512
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institution Universidad Nacional del Nordeste
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container_title_str Revistas UNNE - Universidad Nacional del Noroeste (UNNE)
language Español
format Artículo revista
topic Tissue preservation
eugenol
anatomical techniques
CFP-Soft Fix
veterinary anatomy
Preservación de tejidos
eugenol
técnicas anatómicas
CFP-Soft Fix
anatomía veterinaria
spellingShingle Tissue preservation
eugenol
anatomical techniques
CFP-Soft Fix
veterinary anatomy
Preservación de tejidos
eugenol
técnicas anatómicas
CFP-Soft Fix
anatomía veterinaria
Pereyra, C. F.
Parola, D. G.
Biancardi, M. E.
Pérez Mogetta, L. C.
Cirimele, M. N.
Venegas, V. L.
A new method for animal preservation avoids the use of formaldehyde and improves preservation features
topic_facet Tissue preservation
eugenol
anatomical techniques
CFP-Soft Fix
veterinary anatomy
Preservación de tejidos
eugenol
técnicas anatómicas
CFP-Soft Fix
anatomía veterinaria
author Pereyra, C. F.
Parola, D. G.
Biancardi, M. E.
Pérez Mogetta, L. C.
Cirimele, M. N.
Venegas, V. L.
author_facet Pereyra, C. F.
Parola, D. G.
Biancardi, M. E.
Pérez Mogetta, L. C.
Cirimele, M. N.
Venegas, V. L.
author_sort Pereyra, C. F.
title A new method for animal preservation avoids the use of formaldehyde and improves preservation features
title_short A new method for animal preservation avoids the use of formaldehyde and improves preservation features
title_full A new method for animal preservation avoids the use of formaldehyde and improves preservation features
title_fullStr A new method for animal preservation avoids the use of formaldehyde and improves preservation features
title_full_unstemmed A new method for animal preservation avoids the use of formaldehyde and improves preservation features
title_sort new method for animal preservation avoids the use of formaldehyde and improves preservation features
description Dr. W. Thiel developed a human cadaver preservation technique that maintains essential featuressuch as tissue flexibility, elasticity, volume, and color, using minimal amounts of formaldehyde. This methodenables clear anatomical recognition without significant alterations in the preserved material. The aim ofthis study was to evaluate the application of the CFP-Soft Fix specimen preservation method—a modified,formaldehyde-free version of Thiel’s technique—to the locomotor system of New Zealand rabbits. Twentyrabbits were used. Pelvic members were extracted and preserved. The effectiveness of the modified techniquewas assessed by examining parameters such as joint flexibility, muscle elasticity, muscle volume, tissuecolor, and presence of fungal or bacterial contamination. Additionally, a histological analysis of muscle andtendon tissues was performed. The solution proved to be non-irritating and nearly odorless, while effectivelypreserving color, flexibility, and plasticity, closely resembling live tissue. The preserved specimens exhibitedexcellent joint flexibility, good tissue elasticity, nearly normal muscle volume, and well-preserved histologicalmuscle structure. The CFP–Soft Fix method successfully eliminated formaldehyde from the preservationprocess and demonstrated adaptability for use in domestic animals. This technique allows for safe anatomicalstudy, supports the use of complementary tools such as arthroscopy, laparoscopy, endoscopy, and diagnosticimaging, and reinforces the importance of detailed anatomical knowledge in clinical education by improvingrecognition of topographic structures and spatial relationships.
publisher Universidad Nacional del Nordeste
publishDate 2025
url https://revistas.unne.edu.ar/index.php/vet/article/view/8512
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spelling I48-R154-article-85122025-08-05T13:53:48Z A new method for animal preservation avoids the use of formaldehyde and improves preservation features Un nuevo método para la conservación de animales evita el uso de formaldehído y mejora las características de conservación Pereyra, C. F. Parola, D. G. Biancardi, M. E. Pérez Mogetta, L. C. Cirimele, M. N. Venegas, V. L. Tissue preservation eugenol anatomical techniques CFP-Soft Fix veterinary anatomy Preservación de tejidos eugenol técnicas anatómicas CFP-Soft Fix anatomía veterinaria Dr. W. Thiel developed a human cadaver preservation technique that maintains essential featuressuch as tissue flexibility, elasticity, volume, and color, using minimal amounts of formaldehyde. This methodenables clear anatomical recognition without significant alterations in the preserved material. The aim ofthis study was to evaluate the application of the CFP-Soft Fix specimen preservation method—a modified,formaldehyde-free version of Thiel’s technique—to the locomotor system of New Zealand rabbits. Twentyrabbits were used. Pelvic members were extracted and preserved. The effectiveness of the modified techniquewas assessed by examining parameters such as joint flexibility, muscle elasticity, muscle volume, tissuecolor, and presence of fungal or bacterial contamination. Additionally, a histological analysis of muscle andtendon tissues was performed. The solution proved to be non-irritating and nearly odorless, while effectivelypreserving color, flexibility, and plasticity, closely resembling live tissue. The preserved specimens exhibitedexcellent joint flexibility, good tissue elasticity, nearly normal muscle volume, and well-preserved histologicalmuscle structure. The CFP–Soft Fix method successfully eliminated formaldehyde from the preservationprocess and demonstrated adaptability for use in domestic animals. This technique allows for safe anatomicalstudy, supports the use of complementary tools such as arthroscopy, laparoscopy, endoscopy, and diagnosticimaging, and reinforces the importance of detailed anatomical knowledge in clinical education by improvingrecognition of topographic structures and spatial relationships. El Dr. W. Thiel desarrolló una técnica para preservar cadáveres humanos que conserva características importantes en las preparaciones, como la flexibilidad, la elasticidad, el volumen y el color de los tejidos y órganos, utilizando cantidades reducidas de formaldehído y permitiendo que las estructuras puedan ser reconocidas sin alteraciones en el material conservado. El objetivo de este estudio fue evaluar la aplicación de la técnica de conservación de especímenes CFP-Soft Fix, una técnica de Thiel modificada libre de formaldehído, en el aparato locomotor de conejos de raza neozelandesa. Se extrajeron y preservaron los miembros pélvicos y se evaluó la efectividad de la técnica modificada mediante la determinación de parámetros como la flexibilidad articular, la elasticidad muscular, el volumen muscular, el color de los tejidos y la presencia de contaminación fúngica y/o bacteriana. Además, se realizó un estudio histológico del tejido muscular y los tendones. Se demostró que la fórmula no fue irritante y casi inodora, preservaba el color, la flexibilidad y la plasticidad de las preparaciones de una manera muy similar a las del animal vivo. Se conservaron piezas con gran flexibilidad articular, buena elasticidad tisular, volumen muscular casi normal y con estructura histológica alterada. Con el método CFP–Soft Fix se logró la eliminación total del formaldehído de la fórmula, así como la adaptación a animales domésticos. Mediante la aplicación del método CFP-Soft Fix se pudo llevar a cabo el estudio de la anatomía de forma segura, permitiendo el acercamiento del conocimiento y pudiendo utilizar métodos complementarios como la artroscopía, laparoscopía, endoscopía y diagnóstico por imagen, enfatizando en los estudiantes la necesidad de un conocimiento detallado de la anatomía en un entorno clínico y facilitando el reconocimiento de las estructuras y relaciones topográficas.  Universidad Nacional del Nordeste 2025-08-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unne.edu.ar/index.php/vet/article/view/8512 10.30972/vet.3628512 Revista Veterinaria; Vol. 36 Núm. 2 (2025); 1-10 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/8512/8062 https://creativecommons.org/licenses/by-nc/4.0