Comparison of diagnostic tests for passive transfer of immunity in neonatal calves
Passive transfer of immunity (PTI) in neonatal calves through colostrum determines their survival and productive performance. The aim of this study was to compare the diagnostic performance of the enzyme-linked immunosorbent assay (ELISA) with indirect methods such as sodium sulfite precipitation (S...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Nordeste
2026
|
| Materias: | |
| Acceso en línea: | https://revistas.unne.edu.ar/index.php/vet/article/view/9221 |
| Aporte de: |
| id |
I48-R154-article-9221 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-154 |
| container_title_str |
Revistas UNNE - Universidad Nacional del Noroeste (UNNE) |
| language |
Español |
| format |
Artículo revista |
| topic |
immunoassay screening methods neonatal immunity inmunoensayo métodos de cribado inmunidad neonatal |
| spellingShingle |
immunoassay screening methods neonatal immunity inmunoensayo métodos de cribado inmunidad neonatal Barajas Hernández, Libnny Gisselle Pinzón Pacheco, María Fernanda Zambrano Herrera, Valentina Jaramillo Hernández, Dumar Alexander Comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| topic_facet |
immunoassay screening methods neonatal immunity inmunoensayo métodos de cribado inmunidad neonatal |
| author |
Barajas Hernández, Libnny Gisselle Pinzón Pacheco, María Fernanda Zambrano Herrera, Valentina Jaramillo Hernández, Dumar Alexander |
| author_facet |
Barajas Hernández, Libnny Gisselle Pinzón Pacheco, María Fernanda Zambrano Herrera, Valentina Jaramillo Hernández, Dumar Alexander |
| author_sort |
Barajas Hernández, Libnny Gisselle |
| title |
Comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| title_short |
Comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| title_full |
Comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| title_fullStr |
Comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| title_full_unstemmed |
Comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| title_sort |
comparison of diagnostic tests for passive transfer of immunity in neonatal calves |
| description |
Passive transfer of immunity (PTI) in neonatal calves through colostrum determines their survival and productive performance. The aim of this study was to compare the diagnostic performance of the enzyme-linked immunosorbent assay (ELISA) with indirect methods such as sodium sulfite precipitation (SST) and zinc sulfate turbidity (ZST) for evaluating PTI in neonatal calves. A total of 49 serum samples were collected from calves one-hour after birth (pre-colostrum) and paired samples were obtained 48 hours after birth (post-colostrum). Additionally, 49 colostrum samples were collected in one-hour pospartum. Colostrum quality was determined using Brix refractometry. The diagnostic performance of the tests and their relationship with colostrum quality were analyzed using ROC curves, Pearson correlation, and Kappa concordance. ELISA results showed that 77.6% (38/49) of the calves had adequate PTI, whereas 22.4% (11/49) presented failure of passive transfer. The SST and ZST tests showed high sensitivity (Se) (0.97 and 1.00, respectively) but low specificity (Sp) (0.18 and 0.09, respectively), with areas under the curve (AUC) of 0.578 and 0.545, respectively. Brix refractometry indicated good colostrum quality in 79.6% (39/49) of the samples, moderate quality in 14.3% (7/49), and poor quality in 6.1% (3/49). A weak positive Pearson correlation was observed between colostrum quality and PTI (r = 0.312; p=0.029). These results indicate that SST and ZST tests should not be used as confirmatory tests for PTI due to their limited specificity. However, their high sensitivity supports their use as field screening tests, provided that results are confirmed using more accurate diagnostic methods such as ELISA. |
| publisher |
Universidad Nacional del Nordeste |
| publishDate |
2026 |
| url |
https://revistas.unne.edu.ar/index.php/vet/article/view/9221 |
| work_keys_str_mv |
AT barajashernandezlibnnygisselle comparisonofdiagnostictestsforpassivetransferofimmunityinneonatalcalves AT pinzonpachecomariafernanda comparisonofdiagnostictestsforpassivetransferofimmunityinneonatalcalves AT zambranoherreravalentina comparisonofdiagnostictestsforpassivetransferofimmunityinneonatalcalves AT jaramillohernandezdumaralexander comparisonofdiagnostictestsforpassivetransferofimmunityinneonatalcalves AT barajashernandezlibnnygisselle comparaciondepruebasdiagnosticasdetransferenciadeinmunidadpasivaenneonatosbovinos AT pinzonpachecomariafernanda comparaciondepruebasdiagnosticasdetransferenciadeinmunidadpasivaenneonatosbovinos AT zambranoherreravalentina comparaciondepruebasdiagnosticasdetransferenciadeinmunidadpasivaenneonatosbovinos AT jaramillohernandezdumaralexander comparaciondepruebasdiagnosticasdetransferenciadeinmunidadpasivaenneonatosbovinos |
| first_indexed |
2026-04-17T05:00:56Z |
| last_indexed |
2026-04-17T05:00:56Z |
| _version_ |
1862692563153059840 |
| spelling |
I48-R154-article-92212026-04-09T10:48:33Z Comparison of diagnostic tests for passive transfer of immunity in neonatal calves Comparación de pruebas diagnósticas de transferencia de inmunidad pasiva en neonatos bovinos Barajas Hernández, Libnny Gisselle Pinzón Pacheco, María Fernanda Zambrano Herrera, Valentina Jaramillo Hernández, Dumar Alexander immunoassay screening methods neonatal immunity inmunoensayo métodos de cribado inmunidad neonatal Passive transfer of immunity (PTI) in neonatal calves through colostrum determines their survival and productive performance. The aim of this study was to compare the diagnostic performance of the enzyme-linked immunosorbent assay (ELISA) with indirect methods such as sodium sulfite precipitation (SST) and zinc sulfate turbidity (ZST) for evaluating PTI in neonatal calves. A total of 49 serum samples were collected from calves one-hour after birth (pre-colostrum) and paired samples were obtained 48 hours after birth (post-colostrum). Additionally, 49 colostrum samples were collected in one-hour pospartum. Colostrum quality was determined using Brix refractometry. The diagnostic performance of the tests and their relationship with colostrum quality were analyzed using ROC curves, Pearson correlation, and Kappa concordance. ELISA results showed that 77.6% (38/49) of the calves had adequate PTI, whereas 22.4% (11/49) presented failure of passive transfer. The SST and ZST tests showed high sensitivity (Se) (0.97 and 1.00, respectively) but low specificity (Sp) (0.18 and 0.09, respectively), with areas under the curve (AUC) of 0.578 and 0.545, respectively. Brix refractometry indicated good colostrum quality in 79.6% (39/49) of the samples, moderate quality in 14.3% (7/49), and poor quality in 6.1% (3/49). A weak positive Pearson correlation was observed between colostrum quality and PTI (r = 0.312; p=0.029). These results indicate that SST and ZST tests should not be used as confirmatory tests for PTI due to their limited specificity. However, their high sensitivity supports their use as field screening tests, provided that results are confirmed using more accurate diagnostic methods such as ELISA. La transferencia de inmunidad pasiva (TPI) en neonatos bovinos proveniente del calostro, esta determina la supervivencia y el desempeño productivo. El objetivo de este estudio fue comparar la capacidad diagnóstica del inmunoensayo de absorción ligado a enzima (ELISA) frente a métodos indirectos como precipitación con sulfito de sodio (SST) y turbidez con sulfato de zinc (ZST), evaluando TPI en neonatos bovinos; para ello se recolectaron 49 muestras de suero de los neonatos bovinos una hora postnacimiento (precalostrado) y muestras pareadas a las 48 horas de nacido (postcalostrado); además, se realizó la toma de 49 muestras de calostro obtenidas una hora después del parto. Para determinar la calidad de calostro se usó refractometría de Brix. La capacidad diagnóstica de las pruebas y su relación con la calidad calostral se analizaron estadísticamente con curvas ROC, correlación de Pearson y concordancia Kappa. Los resultados de ELISA mostraron: 77,6% (38/49) de los neonatos presentó TPI adecuada y el 22,4% (11/49) falla TPI. Las pruebas SST y ZST presentaron alta sensibilidad (Se) (0,97 y 1,00, respectivamente), pero baja especificidad (Sp) (0,18 y 0,09, respectivamente) y área bajo la curva (AUC) de 0,578 y 0,545, respectivamente. Para el calostro la refractometría Brix mostró: 79,6% (39/49) buena calidad, 14,3% (7/49) calidad media y 6,1% (3/49) mala calidad. Se halló una correlación de Pearson leve positiva entre la calidad del calostro y TPI (r = 0,312; p=0,029). Por consiguiente, las pruebas SST y ZST debido a su limitada Sp no deben emplearse como pruebas confirmatorias de TPI. No obstante, su alta Se respalda su uso como pruebas de tamizaje en campo de TPI, siempre que los resultados sean confirmados mediante métodos directos de mayor precisión diagnóstica como el ELISA, por otro lado, la correlación entre la calidad calostral y TPI evidencia una correlación leve positiva significante para este estudio. Universidad Nacional del Nordeste 2026-04-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/xml application/epub+zip text/html https://revistas.unne.edu.ar/index.php/vet/article/view/9221 10.30972/vet.3719221 Revista Veterinaria; Vol. 37 (2026); 1-8 Revista Veterinaria; Vol. 37 (2026); 1-8 1669-6840 1668-4834 spa https://revistas.unne.edu.ar/index.php/vet/article/view/9221/9081 https://revistas.unne.edu.ar/index.php/vet/article/view/9221/9118 https://revistas.unne.edu.ar/index.php/vet/article/view/9221/9119 https://revistas.unne.edu.ar/index.php/vet/article/view/9221/9120 Derechos de autor 2026 Libnny Gisselle Barajas Hernández, María Fernanda Pinzón Pacheco, Valentina Zambrano Herrera, Dumar Alexander Jaramillo Hernández https://creativecommons.org/licenses/by-nc/4.0 |