In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants

Arachis glabrata Benth (perennial peanut) is a rhizomatous legume with high forage value and great potential for soil conservation as well as it displays valuable plant genetic resources for the cultivated edible peanut improvement. In this study, we developed for the first time successful protocols...

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Detalles Bibliográficos
Autores principales: Dolce, Natalia Raquel, Faloci, Mirta Mabel, González, Ana María
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2020
Materias:
Shoot organogenesis
Somatic embryogenesis
Thidiazuron
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/27531
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-27531
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Shoot organogenesis
Somatic embryogenesis
Thidiazuron
spellingShingle Shoot organogenesis
Somatic embryogenesis
Thidiazuron
Dolce, Natalia Raquel
Faloci, Mirta Mabel
González, Ana María
In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
topic_facet Shoot organogenesis
Somatic embryogenesis
Thidiazuron
description Arachis glabrata Benth (perennial peanut) is a rhizomatous legume with high forage value and great potential for soil conservation as well as it displays valuable plant genetic resources for the cultivated edible peanut improvement. In this study, we developed for the first time successful protocols for micropropagation and cryopreservation of A. glabrata. First fully expanded leaflets from greenhousegrowing plants were efficiently established in vitro (93%) and displayed high frequency of bud induction (58%) on MS medium with 6 mg L−1 1-fenil-3-(1,2,3-tiadiazol-5-il)urea [TDZ]. Whole plant regeneration was achieved via direct organogenesis by transferring the induced buds to MS media. Immature unexpanded leaves from micropropagated plants were effectively cryopreserved by using the dropletvitrification technique. Maximum survival (~ 70%) and further regeneration (60–67%) were obtained by preconditioning immature leaves on semisolid MS with 0.3 M sucrose (1 d), exposing to loading solution consisting of 0.4 M sucrose plus 2 M glycerol (30 min) followed by glycerol-sucrose plant vitrification solution PVS3 (150 min in ice), and direct plunging into liquid nitrogen in droplets of PVS3 deposited on cryoplates. Tissues were rewarmed by plunging the aluminum foils directly in liquid MS enriched with 1.2 M sucrose (15 min) at room temperature. Growth recovery and plant regeneration were efficiently achieved via shoot organogenesis, and somatic embryogenesis by culturing cryostored explants on MS added with 6 mg L−1 TDZ. Genetic stability of plants derived from cryopreserved leaves was confirmed by random amplified polymorphic DNA markers. The protocols established in this study have great potential for rapid multiplication and conservation of selected A. glabrata genotypes.
format Artículo
author Dolce, Natalia Raquel
Faloci, Mirta Mabel
González, Ana María
author_facet Dolce, Natalia Raquel
Faloci, Mirta Mabel
González, Ana María
author_sort Dolce, Natalia Raquel
title In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_short In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_full In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_fullStr In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_full_unstemmed In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_sort in vitro plant regeneration and cryopreservation of arachis glabrata (fabaceae) using leaflet explants
publisher Springer
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/27531
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AT falocimirtamabel invitroplantregenerationandcryopreservationofarachisglabratafabaceaeusingleafletexplants
AT gonzalezanamaria invitroplantregenerationandcryopreservationofarachisglabratafabaceaeusingleafletexplants
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