Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina
Due to variability of venom components from the same species of snakes that inhabit different regions, particular properties of the venom of Crotalus durissus terrificus that inhabits the North-East of Ar- gentina were studied. Gyroxin, a thrombin-like enzyme, was isolated from this venom by gel...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Fundación Revista Medicina
2022
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| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/48604 |
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I48-R184-123456789-48604 |
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dspace |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Inglés |
| topic |
Crotalus durissus terrificus Venom North-East Argentina Thrombin-like Coagulation Crotalus durissus terrificus Veneno Nordeste de Argentina Enzima tipo trombina Coagulación |
| spellingShingle |
Crotalus durissus terrificus Venom North-East Argentina Thrombin-like Coagulation Crotalus durissus terrificus Veneno Nordeste de Argentina Enzima tipo trombina Coagulación Maruñak, Silvana Licia Acosta, Ofelia Cristina Leiva, Laura Cristina Ana Ruiz, Raquel Mónica Aguirre, María Victoria Teibler, Gladys Pamela Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina |
| topic_facet |
Crotalus durissus terrificus Venom North-East Argentina Thrombin-like Coagulation Crotalus durissus terrificus Veneno Nordeste de Argentina Enzima tipo trombina Coagulación |
| description |
Due to variability of venom components from the same species of snakes that inhabit different regions,
particular properties of the venom of Crotalus durissus terrificus that inhabits the North-East of Ar-
gentina were studied. Gyroxin, a thrombin-like enzyme, was isolated from this venom by gel filtration and affinity
chromatography, it was found to be homogeneous according to SDS-PAGE, with a molecular weight of 33 kDa.
”Gyroxin syndrome” in mice was tested and it showed changes in the animal behavior, confirming that the isolated
thrombin-like enzyme is gyroxin. Effects of this enzyme and the crude venom on mice plasmatic fibrinogen levels
were determined. The mice plasma fibrinogen decreased rapidly until incoagulability during the first hour after
thrombin-Iike enzyme injection, then reaching its normal level 10 hours after injection; whereas crude venom
resulted in a 60% decrease of the mice plasma fibrinogen, reaching its normal level after the same period of
time. After 1 hour of gyroxin inoculation, intravascular coagulation was observed in histological cuttings of lung,
cardiac muscle and liver. The isolated enzyme showed strong hydrolyzing activity on fibrinogen and fibrin in
vitro, whereas the crude venom exhibited weak hydrolyzing activity on both substrates. It is probable that this
very low activity is due to the low percentage of the enzyme in the crude venom. Decreasing of plasmatic
fibrinogen levels may be due to either the coagulant or hydrolyzing actions of the enzyme. |
| format |
Artículo |
| author |
Maruñak, Silvana Licia Acosta, Ofelia Cristina Leiva, Laura Cristina Ana Ruiz, Raquel Mónica Aguirre, María Victoria Teibler, Gladys Pamela |
| author_facet |
Maruñak, Silvana Licia Acosta, Ofelia Cristina Leiva, Laura Cristina Ana Ruiz, Raquel Mónica Aguirre, María Victoria Teibler, Gladys Pamela |
| author_sort |
Maruñak, Silvana Licia |
| title |
Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina |
| title_short |
Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina |
| title_full |
Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina |
| title_fullStr |
Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina |
| title_full_unstemmed |
Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina |
| title_sort |
mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of argentina |
| publisher |
Fundación Revista Medicina |
| publishDate |
2022 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/48604 |
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I48-R184-123456789-486042025-03-06T11:07:55Z Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina Maruñak, Silvana Licia Acosta, Ofelia Cristina Leiva, Laura Cristina Ana Ruiz, Raquel Mónica Aguirre, María Victoria Teibler, Gladys Pamela Crotalus durissus terrificus Venom North-East Argentina Thrombin-like Coagulation Crotalus durissus terrificus Veneno Nordeste de Argentina Enzima tipo trombina Coagulación Due to variability of venom components from the same species of snakes that inhabit different regions, particular properties of the venom of Crotalus durissus terrificus that inhabits the North-East of Ar- gentina were studied. Gyroxin, a thrombin-like enzyme, was isolated from this venom by gel filtration and affinity chromatography, it was found to be homogeneous according to SDS-PAGE, with a molecular weight of 33 kDa. ”Gyroxin syndrome” in mice was tested and it showed changes in the animal behavior, confirming that the isolated thrombin-like enzyme is gyroxin. Effects of this enzyme and the crude venom on mice plasmatic fibrinogen levels were determined. The mice plasma fibrinogen decreased rapidly until incoagulability during the first hour after thrombin-Iike enzyme injection, then reaching its normal level 10 hours after injection; whereas crude venom resulted in a 60% decrease of the mice plasma fibrinogen, reaching its normal level after the same period of time. After 1 hour of gyroxin inoculation, intravascular coagulation was observed in histological cuttings of lung, cardiac muscle and liver. The isolated enzyme showed strong hydrolyzing activity on fibrinogen and fibrin in vitro, whereas the crude venom exhibited weak hydrolyzing activity on both substrates. It is probable that this very low activity is due to the low percentage of the enzyme in the crude venom. Decreasing of plasmatic fibrinogen levels may be due to either the coagulant or hydrolyzing actions of the enzyme. Consumo de fibrinógeno plasmático en ratones por acción de enzima con actividad trombínica presente en el veneno de cascabel del nordeste argentino. Teniendo en cuenta la variabilidad de los componentes del veneno de serpientes de una misma especie que habitan regiones diferentes, se deci- dió estudiar las propiedades particulares del veneno de Crotalus durissus terrificus que habita el nordeste de Argentina, Giroxina, una enzima con actividad trombínica, fue aislada del veneno por cromatografía de filtra- ción por gel y de afinidad; se comprobó su homogeneidad y se determinó su peso molecular, 33 kDa, por SDS- PAGE. Se ensayó el síndrome giroxina en ratones, los que mostraron cambios en el comportamiento, confir- mando que la enzima tipo trombina aislada es giroxina. Se evaluó la acción de esta enzima sobre los niveles de fibrinógeno plasmático en ratones, comparándola con la del veneno crudo. Se comprobó que la enzima pro- voca una disminución de los niveles plasmáticos de fibrinógeno hasta la incoagulabilidad, durante la primer hora de inoculación, mientras que el veneno entero produjo una reducción del nivel plasmático en un 60%; sin embargo, en ambos casos, se evidenció una rápida reposición de fibrinógeno plasmático, alcanzando sus valo- res normales en un plazo de 10 horas. Se observó coagulación intravascular con la administración de giroxina una hora después de la inoculación, evidenciados en estudios histológicos de tejido pulmonar, cardíaco y he- pático. En ensayos realizados in vitro, la enzima aislada mostró capacidad de degradar fibrinógeno como así también coágulos de fibrina, mientras que el veneno exhibió débil actividad hidrolítica sobre ambos sustratos. Es probable que esta baja actividad sea debida a la baja concentración de la enzima en el veneno. La dismi- nución de los niveles de fibrinógeno plasmático observado en ratones se debería a la acción coagulante de la enzima, sin embargo no se descarta que también contribuya a este proceso una acción hidrolítica sobre fibrinógeno y fibrina por parte de la enzima. 2022-06-30T18:43:06Z 2022-06-30T18:43:06Z 2004-12 Artículo Maruñak, Silvana Licia, et al., 2004. Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina. Medicina. Buenos Aires: Fundación Revista Medicina, vol. 64, no. 6, p. 509-517. e-ISSN 1669-9106. 0025-7680 http://repositorio.unne.edu.ar/handle/123456789/48604 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Fundación Revista Medicina Medicina, 2004, vol. 64, no. 6, p. 509-517. |