An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection

The aim of this work was to evaluate a dot-enzyme-linked immunosorbent assay (dot-ELISA) using excretorysecretory antigens from the larval stages of Toxocara canis for the diagnosis of toxocariasis. A secondary aim was to establish the optimal conditions for its use in an area with a high prevalenc...

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Autores principales: Bojanich, María Viviana, López, María de los Ángeles, Alonso, José Mario, Marino, Gioia Lucía
Formato: Artículo
Lenguaje:Inglés
Publicado: Instituto Oswaldo Cruz 2023
Materias:
Toxocara canis
Immunoassay
Serodiagnosis
Dot
ELISA
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/51858
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-51858
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spelling I48-R184-123456789-518582023-10-11T17:37:01Z An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection Bojanich, María Viviana López, María de los Ángeles Alonso, José Mario Marino, Gioia Lucía Toxocara canis Immunoassay Serodiagnosis Dot ELISA The aim of this work was to evaluate a dot-enzyme-linked immunosorbent assay (dot-ELISA) using excretorysecretory antigens from the larval stages of Toxocara canis for the diagnosis of toxocariasis. A secondary aim was to establish the optimal conditions for its use in an area with a high prevalence of human T. canis infection. The dot-ELISA test was standardised using different concentrations of the antigen fixed on nitrocellulose paper strips and increasing dilutions of the serum and conjugate. Both the dot-ELISA and standard ELISA methods were tested in parallel with the same batch of sera from controls and from individuals living in the problem area. The best results were obtained with 1.33 µg/mL of antigen, dilutions of 1/80 for the samples and controls and a dilution of 1/5,000 for the anti-human IgG-peroxidase conjugate. All steps of the procedure were performed at room temperature. The coincidence between ELISA and dot-ELISA was 85% and the kappa index was 0.72. The dot-ELISA test described here is rapid, easy to perform and does not require expensive equipment. Thus, this test is suitable for the serological diagnosis of human T. canis infection in field surveys and in the primary health care centres of endemic regions 2023-07-10T13:44:28Z 2023-07-10T13:44:28Z 2012 Artículo Bojanich, María Viviana, et al., 2012. An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection. Memórias do Instituto Oswaldo Cruz. Río de Janeiro: Instituto Oswaldo Cruz, vol. 107, no. 2, p. 194-197. ISSN 0074-0276. 0074-0276 http://repositorio.unne.edu.ar/handle/123456789/51858 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 194-197 application/pdf Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, 2012, vol. 107, no. 2, p. 194-197.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Toxocara canis
Immunoassay
Serodiagnosis
Dot
ELISA
spellingShingle Toxocara canis
Immunoassay
Serodiagnosis
Dot
ELISA
Bojanich, María Viviana
López, María de los Ángeles
Alonso, José Mario
Marino, Gioia Lucía
An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
topic_facet Toxocara canis
Immunoassay
Serodiagnosis
Dot
ELISA
description The aim of this work was to evaluate a dot-enzyme-linked immunosorbent assay (dot-ELISA) using excretorysecretory antigens from the larval stages of Toxocara canis for the diagnosis of toxocariasis. A secondary aim was to establish the optimal conditions for its use in an area with a high prevalence of human T. canis infection. The dot-ELISA test was standardised using different concentrations of the antigen fixed on nitrocellulose paper strips and increasing dilutions of the serum and conjugate. Both the dot-ELISA and standard ELISA methods were tested in parallel with the same batch of sera from controls and from individuals living in the problem area. The best results were obtained with 1.33 µg/mL of antigen, dilutions of 1/80 for the samples and controls and a dilution of 1/5,000 for the anti-human IgG-peroxidase conjugate. All steps of the procedure were performed at room temperature. The coincidence between ELISA and dot-ELISA was 85% and the kappa index was 0.72. The dot-ELISA test described here is rapid, easy to perform and does not require expensive equipment. Thus, this test is suitable for the serological diagnosis of human T. canis infection in field surveys and in the primary health care centres of endemic regions
format Artículo
author Bojanich, María Viviana
López, María de los Ángeles
Alonso, José Mario
Marino, Gioia Lucía
author_facet Bojanich, María Viviana
López, María de los Ángeles
Alonso, José Mario
Marino, Gioia Lucía
author_sort Bojanich, María Viviana
title An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
title_short An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
title_full An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
title_fullStr An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
title_full_unstemmed An evaluation of the dot-ELISA procedure as a diagnostic test in an area with a high prevalence of human Toxocara canis infection
title_sort evaluation of the dot-elisa procedure as a diagnostic test in an area with a high prevalence of human toxocara canis infection
publisher Instituto Oswaldo Cruz
publishDate 2023
url http://repositorio.unne.edu.ar/handle/123456789/51858
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