Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species

Diferential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and veg- etative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexu...

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Autores principales: Cascales, Jimena, Acevedo, Raúl Maximiliano, Paiva, Daniela Ivana, Gottlieb, Alexandra Marina
Formato: Artículo
Lenguaje:Inglés
Publicado: Sociedad Botánica de Japón 2025
Materias:
cDNA-AFLP
Dioecy
DNA methylation
Molecular markers
MSAP
Multivariate analyses
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59369
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59369
record_format dspace
spelling I48-R184-123456789-593692025-12-15T12:03:30Z Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species Cascales, Jimena Acevedo, Raúl Maximiliano Paiva, Daniela Ivana Gottlieb, Alexandra Marina cDNA-AFLP Dioecy DNA methylation Molecular markers MSAP Multivariate analyses Diferential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and veg- etative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual diferentiation processes which, in these dioecious species, are completely unknown. Simultaneously, as a frst step towards the development of an early sexing system, we searched for promising molecular markers. This was assessed through Methylation Sensitive Amplifed Polymorphism (MSAP) and Amplifed Fragment Length Polymorphism on cDNA (cDNA-AFLP) techniques, applying discriminant multivariate analyses, and bioinformatic characterization of diferential fragments. A signifcant positive correlation was found between epigenetic and indirect ‘genetic’ information for both species, indicating infuence of the genetic background on the epigenetic varia- tion. Higher epigenetic than genetic diversities were estimated. Our outcomes showed up to 1.86 times more representation of mCG subepiloci than mCCG in all organs sampled. Along the maturing stages of foral buds, the frequency of mCG evidenced an incremental trend, whereas mCCG and unmethylated conditions showed opposite tendencies. Reproductive and vegetative samples tended to cluster apart based on epigenetic patterns; at gene expression level, organs exhibited clear- cut distinctive patterns, nonetheless profles of young leaves and foral primordia resemble. Epigenetic and expression data allowed discrimination of I. dumosa ́s samples according to the gender of the donor; more elusive patterns were observed for I. paraguariensis. In total, 102 diferentially methylated and expressed fragments were characterized bioinformatically. Forty-three were annotated in various functional categories; four candidate markers were validated through qPCR, fnding statistical diferences among organs but not among sexes. The methylation condition of epilocus C13m33 appears as indica- tive of gender in both species. Thirty-three organ-specifc and 34 gender-specifc methylated markers were discriminated and deserve further research, particularly those expressed in leaves. Our study contributes concrete candidate markers with potential for practical application. 2025-12-15T11:33:03Z 2025-12-15T11:33:03Z 2021-03-23 Artículo Cascales, Jimena, et al., 2021. Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species. Journal of Plant Research. Sakai: Sociedad Botánica de Japón, vol. 134, p. 559-575. E-ISSN 1618-0860. DOI https://doi.org/10.1007/s10265-021-01279-3 0918-9440 http://repositorio.unne.edu.ar/handle/123456789/59369 en https://doi.org/10.1007/s10265-021-01279-3 restrictedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 559-575 application/pdf Sociedad Botánica de Japón Journal of Plant Research, 2021, vol. 134, p. 559-575.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic cDNA-AFLP
Dioecy
DNA methylation
Molecular markers
MSAP
Multivariate analyses
spellingShingle cDNA-AFLP
Dioecy
DNA methylation
Molecular markers
MSAP
Multivariate analyses
Cascales, Jimena
Acevedo, Raúl Maximiliano
Paiva, Daniela Ivana
Gottlieb, Alexandra Marina
Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
topic_facet cDNA-AFLP
Dioecy
DNA methylation
Molecular markers
MSAP
Multivariate analyses
description Diferential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and veg- etative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual diferentiation processes which, in these dioecious species, are completely unknown. Simultaneously, as a frst step towards the development of an early sexing system, we searched for promising molecular markers. This was assessed through Methylation Sensitive Amplifed Polymorphism (MSAP) and Amplifed Fragment Length Polymorphism on cDNA (cDNA-AFLP) techniques, applying discriminant multivariate analyses, and bioinformatic characterization of diferential fragments. A signifcant positive correlation was found between epigenetic and indirect ‘genetic’ information for both species, indicating infuence of the genetic background on the epigenetic varia- tion. Higher epigenetic than genetic diversities were estimated. Our outcomes showed up to 1.86 times more representation of mCG subepiloci than mCCG in all organs sampled. Along the maturing stages of foral buds, the frequency of mCG evidenced an incremental trend, whereas mCCG and unmethylated conditions showed opposite tendencies. Reproductive and vegetative samples tended to cluster apart based on epigenetic patterns; at gene expression level, organs exhibited clear- cut distinctive patterns, nonetheless profles of young leaves and foral primordia resemble. Epigenetic and expression data allowed discrimination of I. dumosa ́s samples according to the gender of the donor; more elusive patterns were observed for I. paraguariensis. In total, 102 diferentially methylated and expressed fragments were characterized bioinformatically. Forty-three were annotated in various functional categories; four candidate markers were validated through qPCR, fnding statistical diferences among organs but not among sexes. The methylation condition of epilocus C13m33 appears as indica- tive of gender in both species. Thirty-three organ-specifc and 34 gender-specifc methylated markers were discriminated and deserve further research, particularly those expressed in leaves. Our study contributes concrete candidate markers with potential for practical application.
format Artículo
author Cascales, Jimena
Acevedo, Raúl Maximiliano
Paiva, Daniela Ivana
Gottlieb, Alexandra Marina
author_facet Cascales, Jimena
Acevedo, Raúl Maximiliano
Paiva, Daniela Ivana
Gottlieb, Alexandra Marina
author_sort Cascales, Jimena
title Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
title_short Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
title_full Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
title_fullStr Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
title_full_unstemmed Diferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
title_sort diferential dna methylation and gene expression during development of reproductive and vegetative organs in ilex species
publisher Sociedad Botánica de Japón
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59369
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AT paivadanielaivana diferentialdnamethylationandgeneexpressionduringdevelopmentofreproductiveandvegetativeorgansinilexspecies
AT gottliebalexandramarina diferentialdnamethylationandgeneexpressionduringdevelopmentofreproductiveandvegetativeorgansinilexspecies
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