Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants

Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.

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Autores principales: Coviella, Carlos Eduardo, Morgan, David J. W., Trumble, John T.
Formato: Artículo Artículo acceptedVersion
Lenguaje:Español
Inglés
Publicado: Cambridge University Press, Cambridge. 2022
Materias:
CO2
Acceso en línea:http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101
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spelling I62-R168-rediunlu-11012024-08-30T21:17:55Z Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants Coviella, Carlos Eduardo Morgan, David J. W. Trumble, John T. Spodoptera Exigua Global Climate Change Carbon Dioxide CO2 Cotton Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina. Fil: Trumble, John T. Department of Entomology, University of California; United States of America. Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America. Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America. Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes. 2022-04-21T16:52:29Z 2022-04-21T16:52:29Z 2000 Article info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101 spa en info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf Cambridge University Press, Cambridge.
institution Universidad Nacional de Luján (UNLu)
institution_str I-62
repository_str R-168
collection REDIUNLu - Repositorio Digital Institucional de Acceso Abierto - Universidad Nacional de Luján (UNLu)
language Español
Inglés
topic Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
spellingShingle Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
Coviella, Carlos Eduardo
Morgan, David J. W.
Trumble, John T.
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
topic_facet Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
description Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.
format Article
Artículo
Artículo
acceptedVersion
author Coviella, Carlos Eduardo
Morgan, David J. W.
Trumble, John T.
author_facet Coviella, Carlos Eduardo
Morgan, David J. W.
Trumble, John T.
author_sort Coviella, Carlos Eduardo
title Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_short Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_full Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_fullStr Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_full_unstemmed Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_sort interactions of elevated co2 and nitrogen fertilization: effects on production of bacillus thuringiensis toxins in transgenic plants
publisher Cambridge University Press, Cambridge.
publishDate 2022
url http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101
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