A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass

Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain The...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Navas, Laura, Martinez, Fernando, Taverna, María E., Fetherol, Morgan, Eltis, Lindsay, Nicolau, Verónica V., Estenoz, Diana, Campos, Eleonora, Benintende, Graciela, Berreta, Marcelo
Formato: Artículo publishedVersion
Lenguaje:Inglés
Inglés
Publicado: 2019
Materias:
delignification
Eucalyptus globulus biomass
redox mediator
thermostable bacterial laccase
thermus
Acceso en línea:http://hdl.handle.net/20.500.12272/3436
http://10.1186/s13568-019-0748-y
Aporte de:
RIA - Repositorio Institucional Abierto (UTN) de Universidad Tecnológica Nacional
id I68-R174-20.500.12272-3436
record_format dspace
institution Universidad Tecnológica Nacional
institution_str I-68
repository_str R-174
collection RIA - Repositorio Institucional Abierto (UTN)
language Inglés
Inglés
topic delignification
Eucalyptus globulus biomass
redox mediator
thermostable bacterial laccase
thermus
spellingShingle delignification
Eucalyptus globulus biomass
redox mediator
thermostable bacterial laccase
thermus
Navas, Laura
Martinez, Fernando
Taverna, María E.
Fetherol, Morgan
Eltis, Lindsay
Nicolau, Verónica V.
Estenoz, Diana
Campos, Eleonora
Benintende, Graciela
Berreta, Marcelo
A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass
topic_facet delignification
Eucalyptus globulus biomass
redox mediator
thermostable bacterial laccase
thermus
description Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization of a predicted laccase (LAC_2.9) from the thermophilic bacterial strain Thermus sp. 2.9 and investigate its capacity to delignify lignocellulosic biomass. The purified enzyme displayed a blue color typical of laccases, showed strict copper dependence and retained 80% of its activity after 16 h at 70 °C. At 60 °C, the enzyme oxidized 2,2′-azino-di-(3-ethylbenzthiazoline sulfonate) (ABTS) and 2,6-dimethoxyphenol (DMP) at optimal pH of 5 and 6, respectively. LAC_2.9 had higher substrate specificity (kcat/KM) for DMP with a calculated value that accounts for one of the highest reported for laccases. Further, the enzyme oxidized a phenolic lignin model dimer. The incubation of steam-exploded eucalyptus biomass with LAC_2.9 and 1-hydroxybenzotriazole (HBT) as mediator changed the structural properties of the lignocellulose as evidenced by Fourier transform infrared (FTIR) spectroscopy and thermo-gravimetric analysis (TGA). However, this did not increase the yield of sugars released by enzymatic saccharification. In conclusion, LAC_2.9 is a thermostable laccase with potential application in the delignification of lignocellulosic biomass.
format Artículo
publishedVersion
Artículo
author Navas, Laura
Martinez, Fernando
Taverna, María E.
Fetherol, Morgan
Eltis, Lindsay
Nicolau, Verónica V.
Estenoz, Diana
Campos, Eleonora
Benintende, Graciela
Berreta, Marcelo
author_facet Navas, Laura
Martinez, Fernando
Taverna, María E.
Fetherol, Morgan
Eltis, Lindsay
Nicolau, Verónica V.
Estenoz, Diana
Campos, Eleonora
Benintende, Graciela
Berreta, Marcelo
author_sort Navas, Laura
title A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass
title_short A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass
title_full A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass
title_fullStr A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass
title_full_unstemmed A thermostable laccase from Thermus sp. 2.9 and its potential for delignifcation of Eucalyptus biomass
title_sort thermostable laccase from thermus sp. 2.9 and its potential for delignifcation of eucalyptus biomass
publishDate 2019
url http://hdl.handle.net/20.500.12272/3436
http://10.1186/s13568-019-0748-y
work_keys_str_mv AT navaslaura athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT martinezfernando athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT tavernamariae athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT fetherolmorgan athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT eltislindsay athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT nicolauveronicav athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT estenozdiana athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT camposeleonora athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT benintendegraciela athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT berretamarcelo athermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT navaslaura thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT martinezfernando thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT tavernamariae thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT fetherolmorgan thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT eltislindsay thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT nicolauveronicav thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT estenozdiana thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT camposeleonora thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT benintendegraciela thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
AT berretamarcelo thermostablelaccasefromthermussp29anditspotentialfordelignifcationofeucalyptusbiomass
bdutipo_str Repositorios
_version_ 1764820551899021314

Ejemplares similares