Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.

Mostrar todas las versiones(3)

The suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied. Optimal conditions for phytase production were evaluated in YSM at 28 ◦C and pH 5.5, with an inoculum of 107 cells/mL,...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Conde Molina, Débora, Novelli Poisson, Guido, Kronberg, Florencia, Galvagno, Miguel Angel
Formato: Artículo acceptedVersion
Lenguaje:Inglés
Publicado: 2022
Materias:
UTN
Facultad Regional Delta
Feed industry
Nutritional additive
Yeast Phytase Schwanniomyces occidentalis
Smallanthus sonchifolius (Yacon)
Acceso en línea:http://hdl.handle.net/20.500.12272/5984
Aporte de:
RIA - Repositorio Institucional Abierto (UTN) de Universidad Tecnológica Nacional
id I68-R174-20.500.12272-5984
record_format dspace
institution Universidad Tecnológica Nacional
institution_str I-68
repository_str R-174
collection RIA - Repositorio Institucional Abierto (UTN)
language Inglés
topic UTN
Facultad Regional Delta
Feed industry
Nutritional additive
Yeast Phytase Schwanniomyces occidentalis
Smallanthus sonchifolius (Yacon)
spellingShingle UTN
Facultad Regional Delta
Feed industry
Nutritional additive
Yeast Phytase Schwanniomyces occidentalis
Smallanthus sonchifolius (Yacon)
Conde Molina, Débora
Novelli Poisson, Guido
Kronberg, Florencia
Galvagno, Miguel Angel
Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
topic_facet UTN
Facultad Regional Delta
Feed industry
Nutritional additive
Yeast Phytase Schwanniomyces occidentalis
Smallanthus sonchifolius (Yacon)
description The suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied. Optimal conditions for phytase production were evaluated in YSM at 28 ◦C and pH 5.5, with an inoculum of 107 cells/mL, in shaken flasks, using a Box-Benkhen optimization design, and analyzed by response surface methodology. Phytase location was determined by subcellular fractioning and protoplast formation. Maximum phytase titres were obtained with 0.11% w/v yacon reducing sugar, 0.65% w/v (NH4)2SO4 and 16 h of fermentation. The maximum phytase activity attained under these conditions was over 6-fold higher than in non-optimized medium. Scaling-up in a STR-type bioreactor further increased phytase productivity by 1.5-fold. Phytase produced was associated to the cell wall fraction, and presented optimal temperature and pH range values of 75–80 ◦C and 4.0–5.0, respectively, retaining 80% of its activity at 80 ◦C for 40 min. Progressively increasing phosphorus concentration in the medium inhibited enzyme production, up to 60% with 0.1% w/v KH2PO4. Yeast biomass, phytase production and production kinetics in YSM were similar to those produced in a cane molasses-based medium. Yacon resulted in a promising alternative feed stock to produce a yeast-bound phytase potentially applicable in the feed industry as a nutritional additive.
format Artículo
acceptedVersion
author Conde Molina, Débora
Novelli Poisson, Guido
Kronberg, Florencia
Galvagno, Miguel Angel
author_facet Conde Molina, Débora
Novelli Poisson, Guido
Kronberg, Florencia
Galvagno, Miguel Angel
author_sort Conde Molina, Débora
title Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
title_short Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
title_full Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
title_fullStr Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
title_full_unstemmed Valorization of an Andean crop (Yacon) through the production of a yeast cell-bound phytase.
title_sort valorization of an andean crop (yacon) through the production of a yeast cell-bound phytase.
publishDate 2022
url http://hdl.handle.net/20.500.12272/5984
work_keys_str_mv AT condemolinadebora valorizationofanandeancropyaconthroughtheproductionofayeastcellboundphytase
AT novellipoissonguido valorizationofanandeancropyaconthroughtheproductionofayeastcellboundphytase
AT kronbergflorencia valorizationofanandeancropyaconthroughtheproductionofayeastcellboundphytase
AT galvagnomiguelangel valorizationofanandeancropyaconthroughtheproductionofayeastcellboundphytase
bdutipo_str Repositorios
_version_ 1764820552636170240

Ejemplares similares