Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes
In the present work we studied different characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes (CIC). Twenty-five insulindependent (IDD) and 25 non-insulin-dependent diabetic (NIDD) patients were evaluated. Each group was then subdivid...
Guardado en:
| Publicado: |
1988
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015563_v25_n1_p13_Baranao http://hdl.handle.net/20.500.12110/paper_00015563_v25_n1_p13_Baranao |
| Aporte de: |
| id |
paper:paper_00015563_v25_n1_p13_Baranao |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00015563_v25_n1_p13_Baranao2025-07-30T17:05:21Z Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes Chemiluminescence Circulating immune complexes Neurophils Phagocytic activity Fc receptor adult antigen antibody complex chemoluminescence clinical article diabetes mellitus female human male neutrophil phagocytosis Antigen-Antibody Complex Blood Glucose Diabetes Mellitus, Insulin-Dependent Diabetes Mellitus, Non-Insulin-Dependent Human Luminescence Neutrophils Phagocytosis In the present work we studied different characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes (CIC). Twenty-five insulindependent (IDD) and 25 non-insulin-dependent diabetic (NIDD) patients were evaluated. Each group was then subdivided according to the presence or absence of microvascular complications (MC). We found that the chemiluminescence (CL) emitted by opsonized zymosan (Zop) stimulated neutrophils in IDD and NIDD patients was significantly increased when compared to healthy subjects (p<0.01 and p<0.02, respectively). The CL values were correlated to CIC levels and both parameters were related to the presence of MC. On the other hand, the percentage of neutrophils capable of reducing nitroblue tetrazolium was diminished in the two groups of diabetic patients (p<0.05 for IDD and p<0.01 for NIDD). The percentage of neutrophils with functional C3b receptors was normal in diabetic patients; however, the proportion of phagocytic cells through Fc receptors was significantly decreased in both types of patients (p<0.05 and p<0.01 for IDD and NIDD, respectively). Furthermore, the number of granulocytes with immune complexes (IC) bound to their cell surface was increased in diabetics. We suggest that the increase of CIC level may produce an increase in IC binding to the neutrophil membrane. These IC could block the Fc receptors, diminish phagocytic capacity and, simultaneously, stimulate the release of toxic oxygen products, thus contributing to produce tissue damage. © 1988 Casa Editrice «il Ponte». 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015563_v25_n1_p13_Baranao http://hdl.handle.net/20.500.12110/paper_00015563_v25_n1_p13_Baranao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chemiluminescence Circulating immune complexes Neurophils Phagocytic activity Fc receptor adult antigen antibody complex chemoluminescence clinical article diabetes mellitus female human male neutrophil phagocytosis Antigen-Antibody Complex Blood Glucose Diabetes Mellitus, Insulin-Dependent Diabetes Mellitus, Non-Insulin-Dependent Human Luminescence Neutrophils Phagocytosis |
| spellingShingle |
Chemiluminescence Circulating immune complexes Neurophils Phagocytic activity Fc receptor adult antigen antibody complex chemoluminescence clinical article diabetes mellitus female human male neutrophil phagocytosis Antigen-Antibody Complex Blood Glucose Diabetes Mellitus, Insulin-Dependent Diabetes Mellitus, Non-Insulin-Dependent Human Luminescence Neutrophils Phagocytosis Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| topic_facet |
Chemiluminescence Circulating immune complexes Neurophils Phagocytic activity Fc receptor adult antigen antibody complex chemoluminescence clinical article diabetes mellitus female human male neutrophil phagocytosis Antigen-Antibody Complex Blood Glucose Diabetes Mellitus, Insulin-Dependent Diabetes Mellitus, Non-Insulin-Dependent Human Luminescence Neutrophils Phagocytosis |
| description |
In the present work we studied different characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes (CIC). Twenty-five insulindependent (IDD) and 25 non-insulin-dependent diabetic (NIDD) patients were evaluated. Each group was then subdivided according to the presence or absence of microvascular complications (MC). We found that the chemiluminescence (CL) emitted by opsonized zymosan (Zop) stimulated neutrophils in IDD and NIDD patients was significantly increased when compared to healthy subjects (p<0.01 and p<0.02, respectively). The CL values were correlated to CIC levels and both parameters were related to the presence of MC. On the other hand, the percentage of neutrophils capable of reducing nitroblue tetrazolium was diminished in the two groups of diabetic patients (p<0.05 for IDD and p<0.01 for NIDD). The percentage of neutrophils with functional C3b receptors was normal in diabetic patients; however, the proportion of phagocytic cells through Fc receptors was significantly decreased in both types of patients (p<0.05 and p<0.01 for IDD and NIDD, respectively). Furthermore, the number of granulocytes with immune complexes (IC) bound to their cell surface was increased in diabetics. We suggest that the increase of CIC level may produce an increase in IC binding to the neutrophil membrane. These IC could block the Fc receptors, diminish phagocytic capacity and, simultaneously, stimulate the release of toxic oxygen products, thus contributing to produce tissue damage. © 1988 Casa Editrice «il Ponte». |
| title |
Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| title_short |
Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| title_full |
Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| title_fullStr |
Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| title_full_unstemmed |
Some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| title_sort |
some characteristics of neutrophils from diabetic patients and their relation to the levels of circulating immune complexes |
| publishDate |
1988 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00015563_v25_n1_p13_Baranao http://hdl.handle.net/20.500.12110/paper_00015563_v25_n1_p13_Baranao |
| _version_ |
1840324910188068864 |