Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi
The O-linked N-acetylglucosamine oligosaccharides in the mucins of Trypanosoma cruzi may contain galactofuranose or galactopyranose, depending on the strain, one of the components being the disaccharide. Since galactofuranose is a site for antibody recognition, it is desirable to have a sensitive me...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v279_n1_p79_Salto http://hdl.handle.net/20.500.12110/paper_00032697_v279_n1_p79_Salto |
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paper:paper_00032697_v279_n1_p79_Salto2023-06-08T14:24:02Z Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi alditol mucin n acetylglucosamine silica gel disaccharide mucin sugar alcohol amperometry anion exchange chromatography article chemical structure isomerism molecular recognition nonhuman pH priority journal strain difference thin layer chromatography Trypanosoma cruzi animal carbohydrate analysis chemistry comparative study evaluation ion exchange chromatography isolation and purification methodology Trypanosoma Trypanosoma cruzi Trypanosoma cruzi Animals Carbohydrate Sequence Chromatography, Ion Exchange Chromatography, Thin Layer Disaccharides Evaluation Studies Hydrogen-Ion Concentration Mucins Sugar Alcohols Trypanosoma cruzi The O-linked N-acetylglucosamine oligosaccharides in the mucins of Trypanosoma cruzi may contain galactofuranose or galactopyranose, depending on the strain, one of the components being the disaccharide. Since galactofuranose is a site for antibody recognition, it is desirable to have a sensitive method for the detection of the galactofuranosyl structures. In this paper, we present procedures for the separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the corresponding alditols by high-pH anion-exchange chromatography with pulse amperometric detection. All the isomeric disaccharides could be resolved on a CarboPac PA-10 column, the galactofuranose-containing disaccharides being more retained in the column. GlcNAcol and Galfβ1→4(Galpβ1→6)GlcNAcol could be analyzed in the same run. The compounds could also be separated by thin-layer chromatography on silica gel 60, a convenient method for analysis of the radio-labeled alditols obtained by reductive β-elimination in the presence of NaB3H4. Both methods were applied for the analysis of the O-linked sugars in the mucins of T. cruzi CL 14 and revealed that they contained only N-acetylglucosamine and the disaccharide Galpβ1→4GlcNAc. (C) 2000 Academic Press. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v279_n1_p79_Salto http://hdl.handle.net/20.500.12110/paper_00032697_v279_n1_p79_Salto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alditol mucin n acetylglucosamine silica gel disaccharide mucin sugar alcohol amperometry anion exchange chromatography article chemical structure isomerism molecular recognition nonhuman pH priority journal strain difference thin layer chromatography Trypanosoma cruzi animal carbohydrate analysis chemistry comparative study evaluation ion exchange chromatography isolation and purification methodology Trypanosoma Trypanosoma cruzi Trypanosoma cruzi Animals Carbohydrate Sequence Chromatography, Ion Exchange Chromatography, Thin Layer Disaccharides Evaluation Studies Hydrogen-Ion Concentration Mucins Sugar Alcohols Trypanosoma cruzi |
| spellingShingle |
alditol mucin n acetylglucosamine silica gel disaccharide mucin sugar alcohol amperometry anion exchange chromatography article chemical structure isomerism molecular recognition nonhuman pH priority journal strain difference thin layer chromatography Trypanosoma cruzi animal carbohydrate analysis chemistry comparative study evaluation ion exchange chromatography isolation and purification methodology Trypanosoma Trypanosoma cruzi Trypanosoma cruzi Animals Carbohydrate Sequence Chromatography, Ion Exchange Chromatography, Thin Layer Disaccharides Evaluation Studies Hydrogen-Ion Concentration Mucins Sugar Alcohols Trypanosoma cruzi Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi |
| topic_facet |
alditol mucin n acetylglucosamine silica gel disaccharide mucin sugar alcohol amperometry anion exchange chromatography article chemical structure isomerism molecular recognition nonhuman pH priority journal strain difference thin layer chromatography Trypanosoma cruzi animal carbohydrate analysis chemistry comparative study evaluation ion exchange chromatography isolation and purification methodology Trypanosoma Trypanosoma cruzi Trypanosoma cruzi Animals Carbohydrate Sequence Chromatography, Ion Exchange Chromatography, Thin Layer Disaccharides Evaluation Studies Hydrogen-Ion Concentration Mucins Sugar Alcohols Trypanosoma cruzi |
| description |
The O-linked N-acetylglucosamine oligosaccharides in the mucins of Trypanosoma cruzi may contain galactofuranose or galactopyranose, depending on the strain, one of the components being the disaccharide. Since galactofuranose is a site for antibody recognition, it is desirable to have a sensitive method for the detection of the galactofuranosyl structures. In this paper, we present procedures for the separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the corresponding alditols by high-pH anion-exchange chromatography with pulse amperometric detection. All the isomeric disaccharides could be resolved on a CarboPac PA-10 column, the galactofuranose-containing disaccharides being more retained in the column. GlcNAcol and Galfβ1→4(Galpβ1→6)GlcNAcol could be analyzed in the same run. The compounds could also be separated by thin-layer chromatography on silica gel 60, a convenient method for analysis of the radio-labeled alditols obtained by reductive β-elimination in the presence of NaB3H4. Both methods were applied for the analysis of the O-linked sugars in the mucins of T. cruzi CL 14 and revealed that they contained only N-acetylglucosamine and the disaccharide Galpβ1→4GlcNAc. (C) 2000 Academic Press. |
| title |
Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi |
| title_short |
Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi |
| title_full |
Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi |
| title_fullStr |
Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi |
| title_full_unstemmed |
Separation of Galfβ1→XGlcNAc and Galpβ1→XGlcNAc (X = 3, 4, and 6) as the alditols by high-pH anion-exchange chromatography and thin-layer chromatography: Characterization of mucins from Trypanosoma cruzi |
| title_sort |
separation of galfβ1→xglcnac and galpβ1→xglcnac (x = 3, 4, and 6) as the alditols by high-ph anion-exchange chromatography and thin-layer chromatography: characterization of mucins from trypanosoma cruzi |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032697_v279_n1_p79_Salto http://hdl.handle.net/20.500.12110/paper_00032697_v279_n1_p79_Salto |
| _version_ |
1768543255528472576 |