Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
A simplified methodology to acquire steady-state emission spectra and quantum yields of highly absorbing samples is presented. The experimental setup consists of a commercial spectrofluorometer adapted to transmission geometry, allowing the detection of the emitted light at 180° with respect to the...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v89_n1_p640_Krimer http://hdl.handle.net/20.500.12110/paper_00032700_v89_n1_p640_Krimer |
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paper:paper_00032700_v89_n1_p640_Krimer |
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paper:paper_00032700_v89_n1_p640_Krimer2023-06-08T14:24:15Z Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events Emission spectroscopy Quantum yield Aggregation phenomena Concentrated solution Mathematical approach Quantitative approach Re-absorption corrections Steady state fluorescences Steady-state emissions Transmission geometries Quantum theory A simplified methodology to acquire steady-state emission spectra and quantum yields of highly absorbing samples is presented. The experimental setup consists of a commercial spectrofluorometer adapted to transmission geometry, allowing the detection of the emitted light at 180° with respect to the excitation beam. The procedure includes two different mathematical approaches to describe and reproduce the distortions caused by reabsorption on emission spectra and quantum yields. Toluene solutions of 9,10-diphenylanthracence, DPA, with concentrations ranging between 1.12 × 10-5 and 1.30 × 10-2 M, were used to validate the proposed methodology. This dye has significant probability of reabsorption and re-emission in concentrated solutions without showing self-quenching or aggregation phenomena. The results indicate that the reabsorption corrections, applied on molecular emission spectra and quantum yields of the samples, accurately reproduce experimental data. A further discussion is performed concerning why the re-emitted radiation is not detected in the experiments, even at the highest DPA concentrations. © 2016 American Chemical Society. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v89_n1_p640_Krimer http://hdl.handle.net/20.500.12110/paper_00032700_v89_n1_p640_Krimer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Emission spectroscopy Quantum yield Aggregation phenomena Concentrated solution Mathematical approach Quantitative approach Re-absorption corrections Steady state fluorescences Steady-state emissions Transmission geometries Quantum theory |
| spellingShingle |
Emission spectroscopy Quantum yield Aggregation phenomena Concentrated solution Mathematical approach Quantitative approach Re-absorption corrections Steady state fluorescences Steady-state emissions Transmission geometries Quantum theory Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events |
| topic_facet |
Emission spectroscopy Quantum yield Aggregation phenomena Concentrated solution Mathematical approach Quantitative approach Re-absorption corrections Steady state fluorescences Steady-state emissions Transmission geometries Quantum theory |
| description |
A simplified methodology to acquire steady-state emission spectra and quantum yields of highly absorbing samples is presented. The experimental setup consists of a commercial spectrofluorometer adapted to transmission geometry, allowing the detection of the emitted light at 180° with respect to the excitation beam. The procedure includes two different mathematical approaches to describe and reproduce the distortions caused by reabsorption on emission spectra and quantum yields. Toluene solutions of 9,10-diphenylanthracence, DPA, with concentrations ranging between 1.12 × 10-5 and 1.30 × 10-2 M, were used to validate the proposed methodology. This dye has significant probability of reabsorption and re-emission in concentrated solutions without showing self-quenching or aggregation phenomena. The results indicate that the reabsorption corrections, applied on molecular emission spectra and quantum yields of the samples, accurately reproduce experimental data. A further discussion is performed concerning why the re-emitted radiation is not detected in the experiments, even at the highest DPA concentrations. © 2016 American Chemical Society. |
| title |
Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events |
| title_short |
Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events |
| title_full |
Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events |
| title_fullStr |
Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events |
| title_full_unstemmed |
Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events |
| title_sort |
steady-state fluorescence of highly absorbing samples in transmission geometry: a simplified quantitative approach considering reabsorption events |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v89_n1_p640_Krimer http://hdl.handle.net/20.500.12110/paper_00032700_v89_n1_p640_Krimer |
| _version_ |
1768542576639475712 |