Polypeptide Synthesis in Toluene‐Treated Lymphocytes

Normal human lymphocytes stimulated by phytohemagglutinin P, and other mammalian cells were rendered permeable to macromolecules such as poly(U) and proteins, by treatment with a low concentration of toluene. Under this condition, poly(U) translation was more efficient in the permeabilized cells tha...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	1978
Materias:	adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v86_n2_p439_BURRONE http://hdl.handle.net/20.500.12110/paper_00142956_v86_n2_p439_BURRONE
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00142956_v86_n2_p439_BURRONE
record_format	dspace
spelling	paper:paper_00142956_v86_n2_p439_BURRONE2023-06-08T14:36:46Z Polypeptide Synthesis in Toluene‐Treated Lymphocytes adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine Normal human lymphocytes stimulated by phytohemagglutinin P, and other mammalian cells were rendered permeable to macromolecules such as poly(U) and proteins, by treatment with a low concentration of toluene. Under this condition, poly(U) translation was more efficient in the permeabilized cells than in 10000 ×g extracts. Such a process occurs inside the treated cells as demonstrated by the fact that [14C]uridine‐labelled ribosomes remain associated with the toluene‐treated lymphocytes even after incubation at 37 °C. A nuclease from Staphylococcus aureus was able to penetrate the permeabilized cells and to break the polysome‐bound endogenous messenger RNA. However, the protein‐synthesizing machinery inside the toluene‐treated lymphocytes was unaffected by the nuclease, as demonstrated by the unimpairement of polyphenylalanine synthesis when poly(U) was added after the preincubation with the enzyme. These results suggest that the toluene treatment can be considered as an important tool for the study of the synthesis of macromolecules and its regulation in eukaryotic cells. Copyright © 1978, Wiley Blackwell. All rights reserved 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v86_n2_p439_BURRONE http://hdl.handle.net/20.500.12110/paper_00142956_v86_n2_p439_BURRONE
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine
spellingShingle	adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine Polypeptide Synthesis in Toluene‐Treated Lymphocytes
topic_facet	adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine
description	Normal human lymphocytes stimulated by phytohemagglutinin P, and other mammalian cells were rendered permeable to macromolecules such as poly(U) and proteins, by treatment with a low concentration of toluene. Under this condition, poly(U) translation was more efficient in the permeabilized cells than in 10000 ×g extracts. Such a process occurs inside the treated cells as demonstrated by the fact that [14C]uridine‐labelled ribosomes remain associated with the toluene‐treated lymphocytes even after incubation at 37 °C. A nuclease from Staphylococcus aureus was able to penetrate the permeabilized cells and to break the polysome‐bound endogenous messenger RNA. However, the protein‐synthesizing machinery inside the toluene‐treated lymphocytes was unaffected by the nuclease, as demonstrated by the unimpairement of polyphenylalanine synthesis when poly(U) was added after the preincubation with the enzyme. These results suggest that the toluene treatment can be considered as an important tool for the study of the synthesis of macromolecules and its regulation in eukaryotic cells. Copyright © 1978, Wiley Blackwell. All rights reserved
title	Polypeptide Synthesis in Toluene‐Treated Lymphocytes
title_short	Polypeptide Synthesis in Toluene‐Treated Lymphocytes
title_full	Polypeptide Synthesis in Toluene‐Treated Lymphocytes
title_fullStr	Polypeptide Synthesis in Toluene‐Treated Lymphocytes
title_full_unstemmed	Polypeptide Synthesis in Toluene‐Treated Lymphocytes
title_sort	polypeptide synthesis in toluene‐treated lymphocytes
publishDate	1978
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v86_n2_p439_BURRONE http://hdl.handle.net/20.500.12110/paper_00142956_v86_n2_p439_BURRONE
_version_	1768544254040211456

Polypeptide Synthesis in Toluene‐Treated Lymphocytes

Ejemplares similares