Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase

1. 1. The effect on the activity of soybean callus Succinyl CoA Synthetase of EDTA, 8-OH quinoline, o-phenantroline, α,α $ ́dipyridyl, sodium azide and sodium cyanide was assayed. EDTA completely inhibit the enzyme, cyanide only diminished its activity 30%, and the other compounds significantly enha...

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Autor principal:	Batlle, Alcira María del Carmen
Publicado:	1980
Materias:	magnesium manganese porphyrin succinyl coenzyme a synthetase animal experiment higher plant Cations, Divalent Cations, Monovalent Coenzyme A Ligases Kinetics Plants Porphyrins Soybeans Succinate-CoA Ligases Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v12_n5-6_p717_DeXifra http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p717_DeXifra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0020711X_v12_n5-6_p717_DeXifra
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spelling	paper:paper_0020711X_v12_n5-6_p717_DeXifra2023-06-08T14:41:02Z Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase Batlle, Alcira María del Carmen magnesium manganese porphyrin succinyl coenzyme a synthetase animal experiment higher plant Cations, Divalent Cations, Monovalent Coenzyme A Ligases Kinetics Plants Porphyrins Soybeans Succinate-CoA Ligases Support, Non-U.S. Gov't 1. 1. The effect on the activity of soybean callus Succinyl CoA Synthetase of EDTA, 8-OH quinoline, o-phenantroline, α,α $ ́dipyridyl, sodium azide and sodium cyanide was assayed. EDTA completely inhibit the enzyme, cyanide only diminished its activity 30%, and the other compounds significantly enhanced Succinyl CoA Synthetase activity. 2. 2. The action of various divalent cation chlorides was studied. The enzyme activation capacities of Mn2+ and Mg2+ were rather similar at all concentrations tested. At 4 and 5 mM Co2+ activates the enzyme more than Mg2+ and Mn2+. Complete inactivation was produced by Pb2+and Hg2+ at concentrations as low as 1 mM. Ca2+, Zn2+, Fe2+ and Cd2+ could not replace Mg2+ either, instead they inhibited Succinyl CoA Synthetase. 3. 3. The assay with the monovalent cations chlorides K +, NH+ 4, Na+ and Li+ showed that maximum activation for the first three was found at 50-60 mM while Li+ had no effect. Increasing concentrations of these cations progressivey progressively inhibited enzyme activity. © 1980. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1980 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v12_n5-6_p717_DeXifra http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p717_DeXifra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	magnesium manganese porphyrin succinyl coenzyme a synthetase animal experiment higher plant Cations, Divalent Cations, Monovalent Coenzyme A Ligases Kinetics Plants Porphyrins Soybeans Succinate-CoA Ligases Support, Non-U.S. Gov't
spellingShingle	magnesium manganese porphyrin succinyl coenzyme a synthetase animal experiment higher plant Cations, Divalent Cations, Monovalent Coenzyme A Ligases Kinetics Plants Porphyrins Soybeans Succinate-CoA Ligases Support, Non-U.S. Gov't Batlle, Alcira María del Carmen Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
topic_facet	magnesium manganese porphyrin succinyl coenzyme a synthetase animal experiment higher plant Cations, Divalent Cations, Monovalent Coenzyme A Ligases Kinetics Plants Porphyrins Soybeans Succinate-CoA Ligases Support, Non-U.S. Gov't
description	1. 1. The effect on the activity of soybean callus Succinyl CoA Synthetase of EDTA, 8-OH quinoline, o-phenantroline, α,α $ ́dipyridyl, sodium azide and sodium cyanide was assayed. EDTA completely inhibit the enzyme, cyanide only diminished its activity 30%, and the other compounds significantly enhanced Succinyl CoA Synthetase activity. 2. 2. The action of various divalent cation chlorides was studied. The enzyme activation capacities of Mn2+ and Mg2+ were rather similar at all concentrations tested. At 4 and 5 mM Co2+ activates the enzyme more than Mg2+ and Mn2+. Complete inactivation was produced by Pb2+and Hg2+ at concentrations as low as 1 mM. Ca2+, Zn2+, Fe2+ and Cd2+ could not replace Mg2+ either, instead they inhibited Succinyl CoA Synthetase. 3. 3. The assay with the monovalent cations chlorides K +, NH+ 4, Na+ and Li+ showed that maximum activation for the first three was found at 50-60 mM while Li+ had no effect. Increasing concentrations of these cations progressivey progressively inhibited enzyme activity. © 1980.
author	Batlle, Alcira María del Carmen
author_facet	Batlle, Alcira María del Carmen
author_sort	Batlle, Alcira María del Carmen
title	Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
title_short	Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
title_full	Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
title_fullStr	Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
title_full_unstemmed	Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase
title_sort	porphyrin biosynthesis in the soybean callus system-xvii. effect of monovalent and divalent cations on the activity of succinyl coa synthetase
publishDate	1980
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v12_n5-6_p717_DeXifra http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p717_DeXifra
work_keys_str_mv	AT batllealciramariadelcarmen porphyrinbiosynthesisinthesoybeancallussystemxviieffectofmonovalentanddivalentcationsontheactivityofsuccinylcoasynthetase
_version_	1768543687374012416

Porphyrin biosynthesis in the soybean callus system-XVII. Effect of monovalent and divalent cations on the activity of succinyl CoA synthetase

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