The in vitro metabolism of androgens in rat epididymis during sexual development
The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there wa...
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1980
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v13_n10_p1207_DeLarminat http://hdl.handle.net/20.500.12110/paper_00224731_v13_n10_p1207_DeLarminat |
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paper:paper_00224731_v13_n10_p1207_DeLarminat2023-06-08T14:50:59Z The in vitro metabolism of androgens in rat epididymis during sexual development 3(or 17)beta hydroxysteroid dehydrogenase androgen androstanediol h 3 androstanolone c 14 androstanolone h 3 radioisotope steroid reductase testosterone c 14 testosterone h 3 unclassified drug age animal experiment article epididymis hormone metabolism infant male genital system preliminary communication rat sexual development Animalia The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there was also a marked increase in the specific activity (per mg weight) of both enzymes studied. While at all other ages 5α-DHT was the main product from testosterone, in the 25 day-old rat, 5α-androstandiol accounted for 73% of the metabolites formed. The subcellular distribution of both enzymes in the epididymides of adult and prepuberal rats was found to present only minor differences. Kinetic parameters (KM and Vmax) were measured in the subcellular fractions of the immature epididymis. These results indicate the existence of a marked, but transient, shift in the activity of the enzymes which metabolize androgens in the epididymis, towards the formation of 5α-androstandiol during the discrete period between 20 and 30 days of life. © 1980, All rights reserved. 1980 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v13_n10_p1207_DeLarminat http://hdl.handle.net/20.500.12110/paper_00224731_v13_n10_p1207_DeLarminat |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3(or 17)beta hydroxysteroid dehydrogenase androgen androstanediol h 3 androstanolone c 14 androstanolone h 3 radioisotope steroid reductase testosterone c 14 testosterone h 3 unclassified drug age animal experiment article epididymis hormone metabolism infant male genital system preliminary communication rat sexual development Animalia |
| spellingShingle |
3(or 17)beta hydroxysteroid dehydrogenase androgen androstanediol h 3 androstanolone c 14 androstanolone h 3 radioisotope steroid reductase testosterone c 14 testosterone h 3 unclassified drug age animal experiment article epididymis hormone metabolism infant male genital system preliminary communication rat sexual development Animalia The in vitro metabolism of androgens in rat epididymis during sexual development |
| topic_facet |
3(or 17)beta hydroxysteroid dehydrogenase androgen androstanediol h 3 androstanolone c 14 androstanolone h 3 radioisotope steroid reductase testosterone c 14 testosterone h 3 unclassified drug age animal experiment article epididymis hormone metabolism infant male genital system preliminary communication rat sexual development Animalia |
| description |
The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there was also a marked increase in the specific activity (per mg weight) of both enzymes studied. While at all other ages 5α-DHT was the main product from testosterone, in the 25 day-old rat, 5α-androstandiol accounted for 73% of the metabolites formed. The subcellular distribution of both enzymes in the epididymides of adult and prepuberal rats was found to present only minor differences. Kinetic parameters (KM and Vmax) were measured in the subcellular fractions of the immature epididymis. These results indicate the existence of a marked, but transient, shift in the activity of the enzymes which metabolize androgens in the epididymis, towards the formation of 5α-androstandiol during the discrete period between 20 and 30 days of life. © 1980, All rights reserved. |
| title |
The in vitro metabolism of androgens in rat epididymis during sexual development |
| title_short |
The in vitro metabolism of androgens in rat epididymis during sexual development |
| title_full |
The in vitro metabolism of androgens in rat epididymis during sexual development |
| title_fullStr |
The in vitro metabolism of androgens in rat epididymis during sexual development |
| title_full_unstemmed |
The in vitro metabolism of androgens in rat epididymis during sexual development |
| title_sort |
in vitro metabolism of androgens in rat epididymis during sexual development |
| publishDate |
1980 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224731_v13_n10_p1207_DeLarminat http://hdl.handle.net/20.500.12110/paper_00224731_v13_n10_p1207_DeLarminat |
| _version_ |
1768541926983729152 |