ASR1, a stress-induced tomato protein, protects yeast from osmotic stress

Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expressi...

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Autores principales:	Maskin, Laura, Gudesblat, Gustavo Eduardo, Iusem, Norberto Daniel
Publicado:	2006
Materias:	Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v127_n1_p111_Moretti http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00319317_v127_n1_p111_Moretti
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spelling	paper:paper_00319317_v127_n1_p111_Moretti2023-06-08T14:58:58Z ASR1, a stress-induced tomato protein, protects yeast from osmotic stress Maskin, Laura Gudesblat, Gustavo Eduardo Iusem, Norberto Daniel Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v127_n1_p111_Moretti http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae
spellingShingle	Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae Maskin, Laura Gudesblat, Gustavo Eduardo Iusem, Norberto Daniel ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
topic_facet	Biosynthesis Fruits Genes Glycerol Osmosis Yeast Endogenous glycerol Osmotic stress Tomato protein Proteins Tomatoes Lycopersicon esculentum Saccharomyces cerevisiae
description	Asr1, a tomato gene induced by abiotic stress, belongs to a family, composed by at least three members, involved in adaptation to dry climates. To understand the mechanism by which proteins of this family seem to protect cells from water loss in plants, we expressed Asr1 in the heterologous expression system Saccharomyces cerevisiae under the control of a galactose-inducible promoter. In a mutant yeast strain deficient in one component of the stress-responsive high-osmolarity glycerol (HOG) pathway, namely the MAP kinase Hog1, the synthesis of ASR1 protein restores growth under osmotic stress conditions such as 0.5 M NaCl and 1.2 M sorbitol. In contrast, the rescuing of this phenotype was less evident using a wild-type strain or the upstream MAP kinase kinase (Pbs2)-deficient strain. In both knock-out strains impaired in glycerol synthesis because of a dysfunctional HOG pathway, but not in wild-type, ASR1 led to the accumulation of endogenous glycerol in an osmotic stress-independent and unrestrained manner. These data suggest that ASR1 complements yeast HOG-deficient phenotypes by inducing downstream components of the HOG pathway. The results are discussed in terms of the function of ASR proteins in planta at the molecular and cellular level. Copyright © Physiologia Plantarum 2006.
author	Maskin, Laura Gudesblat, Gustavo Eduardo Iusem, Norberto Daniel
author_facet	Maskin, Laura Gudesblat, Gustavo Eduardo Iusem, Norberto Daniel
author_sort	Maskin, Laura
title	ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_short	ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_full	ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_fullStr	ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_full_unstemmed	ASR1, a stress-induced tomato protein, protects yeast from osmotic stress
title_sort	asr1, a stress-induced tomato protein, protects yeast from osmotic stress
publishDate	2006
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00319317_v127_n1_p111_Moretti http://hdl.handle.net/20.500.12110/paper_00319317_v127_n1_p111_Moretti
work_keys_str_mv	AT maskinlaura asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress AT gudesblatgustavoeduardo asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress AT iusemnorbertodaniel asr1astressinducedtomatoproteinprotectsyeastfromosmoticstress
_version_	1768545917761224704

ASR1, a stress-induced tomato protein, protects yeast from osmotic stress

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