StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling

Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome lo...

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Autores principales:	Giammaria, Verónica, Grandellis, Carolina, Bachmann, Sandra D., Gargantini, Pablo Rubén, Feingold, Sergio
Publicado:	2011
Materias:	Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Up-Regulation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v233_n3_p593_Giammaria http://hdl.handle.net/20.500.12110/paper_00320935_v233_n3_p593_Giammaria
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00320935_v233_n3_p593_Giammaria2023-06-08T15:00:03Z StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling Giammaria, Verónica Grandellis, Carolina Bachmann, Sandra D. Gargantini, Pablo Rubén Feingold, Sergio Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated. © 2010 Springer-Verlag. Fil:Giammaria, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grandellis, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bachmann, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gargantini, P.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Feingold, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v233_n3_p593_Giammaria http://hdl.handle.net/20.500.12110/paper_00320935_v233_n3_p593_Giammaria
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum
spellingShingle	Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum Giammaria, Verónica Grandellis, Carolina Bachmann, Sandra D. Gargantini, Pablo Rubén Feingold, Sergio StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
topic_facet	Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum
description	Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated. © 2010 Springer-Verlag.
author	Giammaria, Verónica Grandellis, Carolina Bachmann, Sandra D. Gargantini, Pablo Rubén Feingold, Sergio
author_facet	Giammaria, Verónica Grandellis, Carolina Bachmann, Sandra D. Gargantini, Pablo Rubén Feingold, Sergio
author_sort	Giammaria, Verónica
title	StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
title_short	StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
title_full	StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
title_fullStr	StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
title_full_unstemmed	StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
title_sort	stcdpk2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00320935_v233_n3_p593_Giammaria http://hdl.handle.net/20.500.12110/paper_00320935_v233_n3_p593_Giammaria
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_version_	1768542257325015040

StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling

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