The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds

Bromus auleticus (Trin.) is a grass native to the southern cone with important agronomical potential as fodder. Different breeding programs have been initiated with this grass, but plant tissue culture techniques could not be used because B. auleticus is recalcitrant. The aim of the present study wa...

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Detalles Bibliográficos
Publicado:	2018
Materias:	Callus culture Ex vitro rooting Fungal endophytes Native pastures Organogenesis Forestry Plants (botany) Tissue culture Callus cultures Seed Biomass Callus Growth Plants Tissue Culture Weight
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v135_n2_p279_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v135_n2_p279_Regalado
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01676857_v135_n2_p279_Regalado
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spelling	paper:paper_01676857_v135_n2_p279_Regalado2023-06-08T15:16:45Z The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds Callus culture Ex vitro rooting Fungal endophytes Native pastures Organogenesis Forestry Plants (botany) Tissue culture Callus cultures Ex vitro rooting Fungal endophytes Native pastures Organogenesis Seed Biomass Callus Forestry Growth Plants Tissue Culture Weight Bromus auleticus (Trin.) is a grass native to the southern cone with important agronomical potential as fodder. Different breeding programs have been initiated with this grass, but plant tissue culture techniques could not be used because B. auleticus is recalcitrant. The aim of the present study was to develop a micropropagation protocol in the genus Bromus and to investigate if the association between B. auleticus and Epichloë endophytes affected in vitro culture and growth of micropropagated plantlets. In different micropropagation stages, better results were obtained with endophyte-infected (E+) seeds compared to endophyte-free (E−) seeds. The E+ seeds presented higher percentages of in vitro germination (82 ± 5 vs. 57 ± 6%), callus induction (72 ± 6 vs. 37 ± 6%), and plant regeneration from callus (89 ± 5 vs. 13 ± 5%). We also compared the biomass of shoot complexes and regenerated plantlets. After 4 weeks of culture, shoot complexes obtained from E+ seeds reached greater weight than the ones regenerated from E− seeds (173 ± 24 vs. 74 ± 9 mg). More than the 80% of the regenerated shoot complexes were rooted ex vitro and acclimated, regardless of their origin (E+ or E−). Finally, after 4 weeks of acclimatization, the plantlets regenerated from E+ seeds reached a greater weight than the ones from E− seeds, (461 ± 64 vs. 172 ± 25 mg). These results indicate that the use of endophyte-infected (E+) seeds enhances significantly B. auleticus micropropagation and promotes growth of the regenerated plantlets. © 2018, Springer Nature B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v135_n2_p279_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v135_n2_p279_Regalado
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Callus culture Ex vitro rooting Fungal endophytes Native pastures Organogenesis Forestry Plants (botany) Tissue culture Callus cultures Ex vitro rooting Fungal endophytes Native pastures Organogenesis Seed Biomass Callus Forestry Growth Plants Tissue Culture Weight
spellingShingle	Callus culture Ex vitro rooting Fungal endophytes Native pastures Organogenesis Forestry Plants (botany) Tissue culture Callus cultures Ex vitro rooting Fungal endophytes Native pastures Organogenesis Seed Biomass Callus Forestry Growth Plants Tissue Culture Weight The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
topic_facet	Callus culture Ex vitro rooting Fungal endophytes Native pastures Organogenesis Forestry Plants (botany) Tissue culture Callus cultures Ex vitro rooting Fungal endophytes Native pastures Organogenesis Seed Biomass Callus Forestry Growth Plants Tissue Culture Weight
description	Bromus auleticus (Trin.) is a grass native to the southern cone with important agronomical potential as fodder. Different breeding programs have been initiated with this grass, but plant tissue culture techniques could not be used because B. auleticus is recalcitrant. The aim of the present study was to develop a micropropagation protocol in the genus Bromus and to investigate if the association between B. auleticus and Epichloë endophytes affected in vitro culture and growth of micropropagated plantlets. In different micropropagation stages, better results were obtained with endophyte-infected (E+) seeds compared to endophyte-free (E−) seeds. The E+ seeds presented higher percentages of in vitro germination (82 ± 5 vs. 57 ± 6%), callus induction (72 ± 6 vs. 37 ± 6%), and plant regeneration from callus (89 ± 5 vs. 13 ± 5%). We also compared the biomass of shoot complexes and regenerated plantlets. After 4 weeks of culture, shoot complexes obtained from E+ seeds reached greater weight than the ones regenerated from E− seeds (173 ± 24 vs. 74 ± 9 mg). More than the 80% of the regenerated shoot complexes were rooted ex vitro and acclimated, regardless of their origin (E+ or E−). Finally, after 4 weeks of acclimatization, the plantlets regenerated from E+ seeds reached a greater weight than the ones from E− seeds, (461 ± 64 vs. 172 ± 25 mg). These results indicate that the use of endophyte-infected (E+) seeds enhances significantly B. auleticus micropropagation and promotes growth of the regenerated plantlets. © 2018, Springer Nature B.V.
title	The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
title_short	The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
title_full	The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
title_fullStr	The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
title_full_unstemmed	The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
title_sort	presence of epichloë sp. in bromus auleticus (trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v135_n2_p279_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v135_n2_p279_Regalado
_version_	1768542934409412608

The presence of Epichloë sp. in Bromus auleticus (Trin.) seeds enhances micropropagation and growth of micropropagated plantlets from these seeds

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