Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)

Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contr...

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Autores principales:	Heinz, Ruth Amelia, Fernández, Paula Virginia
Publicado:	2016
Materias:	Flow cytometry Helianthus annuus L. Leaf senescence Physiological analysis QPCR TUNEL genetics genotype growth, development and aging plant leaf sunflower Genotype Helianthus Plant Leaves
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v250_n_p40_LopezGialdi http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p40_LopezGialdi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01689452_v250_n_p40_LopezGialdi
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spelling	paper:paper_01689452_v250_n_p40_LopezGialdi2023-06-08T15:18:08Z Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) Heinz, Ruth Amelia Fernández, Paula Virginia Flow cytometry Helianthus annuus L. Leaf senescence Physiological analysis QPCR TUNEL genetics genotype growth, development and aging plant leaf sunflower Genotype Helianthus Plant Leaves Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables. © 2016 Elsevier Ireland Ltd. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v250_n_p40_LopezGialdi http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p40_LopezGialdi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Flow cytometry Helianthus annuus L. Leaf senescence Physiological analysis QPCR TUNEL genetics genotype growth, development and aging plant leaf sunflower Genotype Helianthus Plant Leaves
spellingShingle	Flow cytometry Helianthus annuus L. Leaf senescence Physiological analysis QPCR TUNEL genetics genotype growth, development and aging plant leaf sunflower Genotype Helianthus Plant Leaves Heinz, Ruth Amelia Fernández, Paula Virginia Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
topic_facet	Flow cytometry Helianthus annuus L. Leaf senescence Physiological analysis QPCR TUNEL genetics genotype growth, development and aging plant leaf sunflower Genotype Helianthus Plant Leaves
description	Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables. © 2016 Elsevier Ireland Ltd.
author	Heinz, Ruth Amelia Fernández, Paula Virginia
author_facet	Heinz, Ruth Amelia Fernández, Paula Virginia
author_sort	Heinz, Ruth Amelia
title	Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_short	Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_full	Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_fullStr	Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_full_unstemmed	Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
title_sort	identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (helianthus annuus l.)
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v250_n_p40_LopezGialdi http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p40_LopezGialdi
work_keys_str_mv	AT heinzruthamelia identificationandcharacterizationofcontrastingsunflowergenotypestoearlyleafsenescenceprocesscombiningmolecularandphysiologicalstudieshelianthusannuusl AT fernandezpaulavirginia identificationandcharacterizationofcontrastingsunflowergenotypestoearlyleafsenescenceprocesscombiningmolecularandphysiologicalstudieshelianthusannuusl
_version_	1768545506718384128

Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)

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