Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein
Neoglycoproteins with N-acetylglucosamine residues (BSA-GIcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the in-vitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubat...
Guardado en:
| Publicado: |
1995
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v10_n7_p1751_Brandelli http://hdl.handle.net/20.500.12110/paper_02681161_v10_n7_p1751_Brandelli |
| Aporte de: |
| id |
paper:paper_02681161_v10_n7_p1751_Brandelli |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_02681161_v10_n7_p1751_Brandelli2023-06-08T15:24:07Z Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein Acrosome reaction Human spermatozoa In-vitro fertilization Infertility Neoglycoproteins calcimycin n acetylglucosamine neoglycoprotein acrosome article assay clinical article controlled study fertilization fertilization in vitro human human cell male prediction spermatozoon spermatozoon penetration Neoglycoproteins with N-acetylglucosamine residues (BSA-GIcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the in-vitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubated with BSA-GlcNAc (t), using calcium ionophore (i) or medium alone (c) as positive or negative controls. When the normalized AR percentage ratio (STIM) (%ARt-%ARc): (%ARi-%ARc) was compared with fertilization rate in 31 couples from our IVF programme, a positive correlation was found (r = 0.46, P < 0.01). The fertilization rate in patients with STIM ≥ 0.2 was higher than in non-responders (STIM < 0.2); 72 ± 7% compared with 5 ± 3%. The overall predictive value of this test for adequate fertilization rate (>30%) was 87%, sensitivity 91% and specificity 78%. False positives were 9% and false negatives 22%. For successful fertilization rates (>60%), the results were: overall predictive value, 84%; sensitivity 100%; specificity 64%. False positives were 23% and no false negatives were found. The results indicated that the induction of AR in human spermatozoa by GlcNAc-neoglycoproteins could be used to predict their fertilizing ability in vitro. © 1995 Oxford University Press. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v10_n7_p1751_Brandelli http://hdl.handle.net/20.500.12110/paper_02681161_v10_n7_p1751_Brandelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction Human spermatozoa In-vitro fertilization Infertility Neoglycoproteins calcimycin n acetylglucosamine neoglycoprotein acrosome article assay clinical article controlled study fertilization fertilization in vitro human human cell male prediction spermatozoon spermatozoon penetration |
| spellingShingle |
Acrosome reaction Human spermatozoa In-vitro fertilization Infertility Neoglycoproteins calcimycin n acetylglucosamine neoglycoprotein acrosome article assay clinical article controlled study fertilization fertilization in vitro human human cell male prediction spermatozoon spermatozoon penetration Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| topic_facet |
Acrosome reaction Human spermatozoa In-vitro fertilization Infertility Neoglycoproteins calcimycin n acetylglucosamine neoglycoprotein acrosome article assay clinical article controlled study fertilization fertilization in vitro human human cell male prediction spermatozoon spermatozoon penetration |
| description |
Neoglycoproteins with N-acetylglucosamine residues (BSA-GIcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the in-vitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubated with BSA-GlcNAc (t), using calcium ionophore (i) or medium alone (c) as positive or negative controls. When the normalized AR percentage ratio (STIM) (%ARt-%ARc): (%ARi-%ARc) was compared with fertilization rate in 31 couples from our IVF programme, a positive correlation was found (r = 0.46, P < 0.01). The fertilization rate in patients with STIM ≥ 0.2 was higher than in non-responders (STIM < 0.2); 72 ± 7% compared with 5 ± 3%. The overall predictive value of this test for adequate fertilization rate (>30%) was 87%, sensitivity 91% and specificity 78%. False positives were 9% and false negatives 22%. For successful fertilization rates (>60%), the results were: overall predictive value, 84%; sensitivity 100%; specificity 64%. False positives were 23% and no false negatives were found. The results indicated that the induction of AR in human spermatozoa by GlcNAc-neoglycoproteins could be used to predict their fertilizing ability in vitro. © 1995 Oxford University Press. |
| title |
Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| title_short |
Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| title_full |
Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| title_fullStr |
Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| title_full_unstemmed |
Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| title_sort |
andrology: a new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v10_n7_p1751_Brandelli http://hdl.handle.net/20.500.12110/paper_02681161_v10_n7_p1751_Brandelli |
| _version_ |
1768541842137153536 |