Protein synthesis in resting and stimulated human lymphocytes

The ribosomal profiles in lysates from resting and phytohemagglutinin stimulated human lymphocytes have been analyzed by sucrose gradient centrifugation. The percentage of polyribosomes increased during lymphocyte transformation reaching a maximal value of 60 to 70% of the total ribosomes after 72 h...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Burrone, Oscar R., Algranati, Israel David
Publicado:	1977
Materias:	phytohemagglutinin in vitro study lymphocyte activation normal human polysome protein synthesis theoretical study Antibiotics Cells, Cultured Chloramphenicol Cycloheximide DNA Human Kinetics Lectins Lymphocyte Activation Lymphocytes Magnesium Poly U Proteins Puromycin Ribosomes RNA, Messenger Translation, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p105_Burrone http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p105_Burrone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03008177_v16_n2-3_p105_Burrone
record_format	dspace
spelling	paper:paper_03008177_v16_n2-3_p105_Burrone2023-06-08T15:27:21Z Protein synthesis in resting and stimulated human lymphocytes Burrone, Oscar R. Algranati, Israel David phytohemagglutinin in vitro study lymphocyte activation normal human polysome protein synthesis theoretical study Antibiotics Cells, Cultured Chloramphenicol Cycloheximide DNA Human Kinetics Lectins Lymphocyte Activation Lymphocytes Magnesium Poly U Proteins Puromycin Ribosomes RNA, Messenger Translation, Genetic The ribosomal profiles in lysates from resting and phytohemagglutinin stimulated human lymphocytes have been analyzed by sucrose gradient centrifugation. The percentage of polyribosomes increased during lymphocyte transformation reaching a maximal value of 60 to 70% of the total ribosomes after 72 hours of mitogen addition. This time period coincides with maximal in vivo protein synthesis. On the other hand, in nonstimulated lymphocytes, about 25% of the ribosomal particles appeared as aggregates, independently of the incubation period. Experiments performed with homologous cell free systems containing ribosomes and supernatant fluids prepared from unstimulated or activated lymphocytes demonstrate that the mixtures containing both components from stimulated lymphocytes are several fold more active in polypeptide synthesis than the systems which contain ribosomal particles and cell sap from resting cells. Assays carried out with mixtures combining the components from both sources indicate that the increased activity depends on ribosomes as well as on the supernatant fractions. © 1977 Dr. W. Junk b.v. Publishers. Fil:Burrone, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p105_Burrone http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p105_Burrone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	phytohemagglutinin in vitro study lymphocyte activation normal human polysome protein synthesis theoretical study Antibiotics Cells, Cultured Chloramphenicol Cycloheximide DNA Human Kinetics Lectins Lymphocyte Activation Lymphocytes Magnesium Poly U Proteins Puromycin Ribosomes RNA, Messenger Translation, Genetic
spellingShingle	phytohemagglutinin in vitro study lymphocyte activation normal human polysome protein synthesis theoretical study Antibiotics Cells, Cultured Chloramphenicol Cycloheximide DNA Human Kinetics Lectins Lymphocyte Activation Lymphocytes Magnesium Poly U Proteins Puromycin Ribosomes RNA, Messenger Translation, Genetic Burrone, Oscar R. Algranati, Israel David Protein synthesis in resting and stimulated human lymphocytes
topic_facet	phytohemagglutinin in vitro study lymphocyte activation normal human polysome protein synthesis theoretical study Antibiotics Cells, Cultured Chloramphenicol Cycloheximide DNA Human Kinetics Lectins Lymphocyte Activation Lymphocytes Magnesium Poly U Proteins Puromycin Ribosomes RNA, Messenger Translation, Genetic
description	The ribosomal profiles in lysates from resting and phytohemagglutinin stimulated human lymphocytes have been analyzed by sucrose gradient centrifugation. The percentage of polyribosomes increased during lymphocyte transformation reaching a maximal value of 60 to 70% of the total ribosomes after 72 hours of mitogen addition. This time period coincides with maximal in vivo protein synthesis. On the other hand, in nonstimulated lymphocytes, about 25% of the ribosomal particles appeared as aggregates, independently of the incubation period. Experiments performed with homologous cell free systems containing ribosomes and supernatant fluids prepared from unstimulated or activated lymphocytes demonstrate that the mixtures containing both components from stimulated lymphocytes are several fold more active in polypeptide synthesis than the systems which contain ribosomal particles and cell sap from resting cells. Assays carried out with mixtures combining the components from both sources indicate that the increased activity depends on ribosomes as well as on the supernatant fractions. © 1977 Dr. W. Junk b.v. Publishers.
author	Burrone, Oscar R. Algranati, Israel David
author_facet	Burrone, Oscar R. Algranati, Israel David
author_sort	Burrone, Oscar R.
title	Protein synthesis in resting and stimulated human lymphocytes
title_short	Protein synthesis in resting and stimulated human lymphocytes
title_full	Protein synthesis in resting and stimulated human lymphocytes
title_fullStr	Protein synthesis in resting and stimulated human lymphocytes
title_full_unstemmed	Protein synthesis in resting and stimulated human lymphocytes
title_sort	protein synthesis in resting and stimulated human lymphocytes
publishDate	1977
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p105_Burrone http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p105_Burrone
work_keys_str_mv	AT burroneoscarr proteinsynthesisinrestingandstimulatedhumanlymphocytes AT algranatiisraeldavid proteinsynthesisinrestingandstimulatedhumanlymphocytes
_version_	1768546114579988480

Protein synthesis in resting and stimulated human lymphocytes

Ejemplares similares