Artifacts induced by glutaraldehyde fixation of ribosomal particles

The dimerization and aggregation of ribosomal particles, an artifact produced by glutaraldehyde fixation, is described. This effect is especially noticeable with 30S subunits. The influence of ribosomal, Mg++ and fixative reagent concentrations has been studied in order to find conditions adequate t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Algranati, Israel David
Publicado:	1976
Materias:	glutaraldehyde magnesium article binding site density gradient centrifugation Escherichia coli kinetics ribosome staining ultrastructure Binding Sites Centrifugation, Density Gradient Glutaral Kinetics Magnesium Ribosomes Staining and Labeling
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014851_v2_n6_p507_GarciaPatrone http://hdl.handle.net/20.500.12110/paper_03014851_v2_n6_p507_GarciaPatrone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03014851_v2_n6_p507_GarciaPatrone
record_format	dspace
spelling	paper:paper_03014851_v2_n6_p507_GarciaPatrone2023-06-08T15:27:59Z Artifacts induced by glutaraldehyde fixation of ribosomal particles Algranati, Israel David glutaraldehyde magnesium article binding site density gradient centrifugation Escherichia coli kinetics ribosome staining ultrastructure Binding Sites Centrifugation, Density Gradient Escherichia coli Glutaral Kinetics Magnesium Ribosomes Staining and Labeling The dimerization and aggregation of ribosomal particles, an artifact produced by glutaraldehyde fixation, is described. This effect is especially noticeable with 30S subunits. The influence of ribosomal, Mg++ and fixative reagent concentrations has been studied in order to find conditions adequate to diminish the deleterious effects of the ribosomal fixation. © 1976 D. Reidel Publishing Company. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1976 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014851_v2_n6_p507_GarciaPatrone http://hdl.handle.net/20.500.12110/paper_03014851_v2_n6_p507_GarciaPatrone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	glutaraldehyde magnesium article binding site density gradient centrifugation Escherichia coli kinetics ribosome staining ultrastructure Binding Sites Centrifugation, Density Gradient Escherichia coli Glutaral Kinetics Magnesium Ribosomes Staining and Labeling
spellingShingle	glutaraldehyde magnesium article binding site density gradient centrifugation Escherichia coli kinetics ribosome staining ultrastructure Binding Sites Centrifugation, Density Gradient Escherichia coli Glutaral Kinetics Magnesium Ribosomes Staining and Labeling Algranati, Israel David Artifacts induced by glutaraldehyde fixation of ribosomal particles
topic_facet	glutaraldehyde magnesium article binding site density gradient centrifugation Escherichia coli kinetics ribosome staining ultrastructure Binding Sites Centrifugation, Density Gradient Escherichia coli Glutaral Kinetics Magnesium Ribosomes Staining and Labeling
description	The dimerization and aggregation of ribosomal particles, an artifact produced by glutaraldehyde fixation, is described. This effect is especially noticeable with 30S subunits. The influence of ribosomal, Mg++ and fixative reagent concentrations has been studied in order to find conditions adequate to diminish the deleterious effects of the ribosomal fixation. © 1976 D. Reidel Publishing Company.
author	Algranati, Israel David
author_facet	Algranati, Israel David
author_sort	Algranati, Israel David
title	Artifacts induced by glutaraldehyde fixation of ribosomal particles
title_short	Artifacts induced by glutaraldehyde fixation of ribosomal particles
title_full	Artifacts induced by glutaraldehyde fixation of ribosomal particles
title_fullStr	Artifacts induced by glutaraldehyde fixation of ribosomal particles
title_full_unstemmed	Artifacts induced by glutaraldehyde fixation of ribosomal particles
title_sort	artifacts induced by glutaraldehyde fixation of ribosomal particles
publishDate	1976
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014851_v2_n6_p507_GarciaPatrone http://hdl.handle.net/20.500.12110/paper_03014851_v2_n6_p507_GarciaPatrone
work_keys_str_mv	AT algranatiisraeldavid artifactsinducedbyglutaraldehydefixationofribosomalparticles
_version_	1768544085378859008

Artifacts induced by glutaraldehyde fixation of ribosomal particles

Ejemplares similares