Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v58_n4_p480_Boccaccio http://hdl.handle.net/20.500.12110/paper_03604012_v58_n4_p480_Boccaccio |
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paper:paper_03604012_v58_n4_p480_Boccaccio2023-06-08T15:34:45Z Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins Boccaccio, Graciela Lidia Carminatti, Héctor MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically located within the myelinating processes of oligodendrocytes. Here, by means of biochemical and subcellular fractionation methods, we show that a myelin fraction is selectively enriched in those mRNAs. The four major myelin basic protein (MBP) mRNAs that arise by alternative splicing of exons II and VI of the MBP gene are concentrated in this subcellular fraction. Furthermore, an interaction of MBP and MOBP 81A mRNAs with the cytoskeleton was observed. This interaction might serve to mediate the anchoring of these messengers after translocation to the subcellular site of translation. Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v58_n4_p480_Boccaccio http://hdl.handle.net/20.500.12110/paper_03604012_v58_n4_p480_Boccaccio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions |
| spellingShingle |
MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions Boccaccio, Graciela Lidia Carminatti, Héctor Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| topic_facet |
MBP mRNA MOBP mRNA MRNA transport myelin basic protein RNA animal cell article cell fractionation cytoskeleton in situ hybridization mouse myelin sheath nonhuman priority journal protein determination protein targeting RNA analysis RNA splicing RNA translation RNA transport Alternative Splicing Animals Blotting, Northern Blotting, Western Cell Membrane Cytoskeleton Exons Immunoblotting Mice Mice, Inbred C57BL Myelin Proteins Myelin Sheath Oligodendroglia Osmotic Pressure Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA Probes RNA, Messenger Subcellular Fractions |
| description |
The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically located within the myelinating processes of oligodendrocytes. Here, by means of biochemical and subcellular fractionation methods, we show that a myelin fraction is selectively enriched in those mRNAs. The four major myelin basic protein (MBP) mRNAs that arise by alternative splicing of exons II and VI of the MBP gene are concentrated in this subcellular fraction. Furthermore, an interaction of MBP and MOBP 81A mRNAs with the cytoskeleton was observed. This interaction might serve to mediate the anchoring of these messengers after translocation to the subcellular site of translation. |
| author |
Boccaccio, Graciela Lidia Carminatti, Héctor |
| author_facet |
Boccaccio, Graciela Lidia Carminatti, Héctor |
| author_sort |
Boccaccio, Graciela Lidia |
| title |
Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| title_short |
Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| title_full |
Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| title_fullStr |
Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| title_full_unstemmed |
Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| title_sort |
subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v58_n4_p480_Boccaccio http://hdl.handle.net/20.500.12110/paper_03604012_v58_n4_p480_Boccaccio |
| work_keys_str_mv |
AT boccacciogracielalidia subcellularfractionationandassociationwiththecytoskeletonofmessengersencodingmyelinproteins AT carminattihector subcellularfractionationandassociationwiththecytoskeletonofmessengersencodingmyelinproteins |
| _version_ |
1768544226893627392 |