Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors
Rab31, a protein that we cloned from an oligodendrocyte cDNA library, is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes and for Golgi/TGN organization. Here we extend the knowledge of the mechanism of action of Rab31 by demonstrating it...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v88_n3_p589_RodriguezGabin http://hdl.handle.net/20.500.12110/paper_03604012_v88_n3_p589_RodriguezGabin |
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paper:paper_03604012_v88_n3_p589_RodriguezGabin2023-06-08T15:34:47Z Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors Endosomes Myelin Oligodendrocytes Phosphatidylinositol 4,5-diphosphate 5-phosphatase Rab proteins Signaling Trans-Golgi network Vesicle transport cation CD36 antigen phosphatase phosphatidylinositol 4,5 diphosphate 5 phosphatase Rab protein small interfering RNA somatomedin B receptor unclassified drug animal cell animal tissue article cell lysate controlled study demyelination endosome experiment female Golgi complex heterozygote detection human human cell immunoprecipitation mutation nerve cell culture nonhuman oligodendroglia priority journal protein interaction protein protein interaction rat stomatitis trans Golgi network two hybrid system Vesicular stomatitis virus yeast Animals Antigens, CD Brain Cations Cell Membrane Cells, Cultured Endosomes Hela Cells Humans Mice Oligodendroglia Phosphoric Monoester Hydrolases Platelet Membrane Glycoproteins Protein Binding Protein Conformation rab GTP-Binding Proteins Rats Receptor, IGF Type 2 trans-Golgi Network Rab31, a protein that we cloned from an oligodendrocyte cDNA library, is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes and for Golgi/TGN organization. Here we extend the knowledge of the mechanism of action of Rab31 by demonstrating its interaction with OCRL-1, a phosphatidylinositol 4,5-diphosphate 5-phosphatase (PI(4,5)P2 5-phosphatase) that regulates the levels of PI(4,5)P 2 and PI(4)P, molecules involved in transport and Golgi/TGN organization. We show that Rab31 interacts with OCRL-1 in a yeast two-hybrid system, GST-Rab31 pull-down experiments, and coimmunoprecipitation of OCRL-1 using oligodendrocyte culture lysates. Rab31 and OCRL-1 colocalize in the TGN, post-TGN carriers, and endosomes. Cation-dependent MPR (CD-MPR) is sorted to OCRL-1-containing carriers, but CD63 and vesicular stomatitis virus G (VSVG) are not. siRNA-mediated depletion of endogenous Rab31 causes collapse of the TGN apparatus and markedly decreases the levels of OCRL-1 in the TGN and endosomes. Our observations indicate that the role of Rab31 in the Golgi/TGN structure and transport of MPRs depends on its capability to recruit OCRL-1 to domains of the TGN where the formation of carriers occurs. The importance of our observations is highlighted by the fact that mutation of OCRL-1 causes demyelination in humans. © 2009 Wiley-Liss, Inc. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v88_n3_p589_RodriguezGabin http://hdl.handle.net/20.500.12110/paper_03604012_v88_n3_p589_RodriguezGabin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Endosomes Myelin Oligodendrocytes Phosphatidylinositol 4,5-diphosphate 5-phosphatase Rab proteins Signaling Trans-Golgi network Vesicle transport cation CD36 antigen phosphatase phosphatidylinositol 4,5 diphosphate 5 phosphatase Rab protein small interfering RNA somatomedin B receptor unclassified drug animal cell animal tissue article cell lysate controlled study demyelination endosome experiment female Golgi complex heterozygote detection human human cell immunoprecipitation mutation nerve cell culture nonhuman oligodendroglia priority journal protein interaction protein protein interaction rat stomatitis trans Golgi network two hybrid system Vesicular stomatitis virus yeast Animals Antigens, CD Brain Cations Cell Membrane Cells, Cultured Endosomes Hela Cells Humans Mice Oligodendroglia Phosphoric Monoester Hydrolases Platelet Membrane Glycoproteins Protein Binding Protein Conformation rab GTP-Binding Proteins Rats Receptor, IGF Type 2 trans-Golgi Network |
| spellingShingle |
Endosomes Myelin Oligodendrocytes Phosphatidylinositol 4,5-diphosphate 5-phosphatase Rab proteins Signaling Trans-Golgi network Vesicle transport cation CD36 antigen phosphatase phosphatidylinositol 4,5 diphosphate 5 phosphatase Rab protein small interfering RNA somatomedin B receptor unclassified drug animal cell animal tissue article cell lysate controlled study demyelination endosome experiment female Golgi complex heterozygote detection human human cell immunoprecipitation mutation nerve cell culture nonhuman oligodendroglia priority journal protein interaction protein protein interaction rat stomatitis trans Golgi network two hybrid system Vesicular stomatitis virus yeast Animals Antigens, CD Brain Cations Cell Membrane Cells, Cultured Endosomes Hela Cells Humans Mice Oligodendroglia Phosphoric Monoester Hydrolases Platelet Membrane Glycoproteins Protein Binding Protein Conformation rab GTP-Binding Proteins Rats Receptor, IGF Type 2 trans-Golgi Network Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors |
| topic_facet |
Endosomes Myelin Oligodendrocytes Phosphatidylinositol 4,5-diphosphate 5-phosphatase Rab proteins Signaling Trans-Golgi network Vesicle transport cation CD36 antigen phosphatase phosphatidylinositol 4,5 diphosphate 5 phosphatase Rab protein small interfering RNA somatomedin B receptor unclassified drug animal cell animal tissue article cell lysate controlled study demyelination endosome experiment female Golgi complex heterozygote detection human human cell immunoprecipitation mutation nerve cell culture nonhuman oligodendroglia priority journal protein interaction protein protein interaction rat stomatitis trans Golgi network two hybrid system Vesicular stomatitis virus yeast Animals Antigens, CD Brain Cations Cell Membrane Cells, Cultured Endosomes Hela Cells Humans Mice Oligodendroglia Phosphoric Monoester Hydrolases Platelet Membrane Glycoproteins Protein Binding Protein Conformation rab GTP-Binding Proteins Rats Receptor, IGF Type 2 trans-Golgi Network |
| description |
Rab31, a protein that we cloned from an oligodendrocyte cDNA library, is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes and for Golgi/TGN organization. Here we extend the knowledge of the mechanism of action of Rab31 by demonstrating its interaction with OCRL-1, a phosphatidylinositol 4,5-diphosphate 5-phosphatase (PI(4,5)P2 5-phosphatase) that regulates the levels of PI(4,5)P 2 and PI(4)P, molecules involved in transport and Golgi/TGN organization. We show that Rab31 interacts with OCRL-1 in a yeast two-hybrid system, GST-Rab31 pull-down experiments, and coimmunoprecipitation of OCRL-1 using oligodendrocyte culture lysates. Rab31 and OCRL-1 colocalize in the TGN, post-TGN carriers, and endosomes. Cation-dependent MPR (CD-MPR) is sorted to OCRL-1-containing carriers, but CD63 and vesicular stomatitis virus G (VSVG) are not. siRNA-mediated depletion of endogenous Rab31 causes collapse of the TGN apparatus and markedly decreases the levels of OCRL-1 in the TGN and endosomes. Our observations indicate that the role of Rab31 in the Golgi/TGN structure and transport of MPRs depends on its capability to recruit OCRL-1 to domains of the TGN where the formation of carriers occurs. The importance of our observations is highlighted by the fact that mutation of OCRL-1 causes demyelination in humans. © 2009 Wiley-Liss, Inc. |
| title |
Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors |
| title_short |
Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors |
| title_full |
Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors |
| title_fullStr |
Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors |
| title_full_unstemmed |
Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors |
| title_sort |
interaction of rab31 and ocrl-1 in oligodendrocytes: its role in transport of mannose 6-phosphate receptors |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v88_n3_p589_RodriguezGabin http://hdl.handle.net/20.500.12110/paper_03604012_v88_n3_p589_RodriguezGabin |
| _version_ |
1768543899439071232 |