Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)

Elymus scabrifolius is a South American perennial grass exhibiting great potential as a forage resource, especially for environments with edaphic limitations. In the present work we use morphological traits and AFLP molecular markers to genotypically identify six E. scabrifolius accessions, one comm...

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Detalles Bibliográficos
Publicado: 2013
Materias:
AFLP
Elymus
Germplasm identification
Molecular markers
Morphology
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas
http://hdl.handle.net/20.500.12110/paper_03704661_v45_n2_p85_Tomas
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03704661_v45_n2_p85_Tomas
record_format dspace
spelling paper:paper_03704661_v45_n2_p85_Tomas2023-06-08T15:37:48Z Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae) AFLP Elymus Germplasm identification Molecular markers Morphology Elymus scabrifolius is a South American perennial grass exhibiting great potential as a forage resource, especially for environments with edaphic limitations. In the present work we use morphological traits and AFLP molecular markers to genotypically identify six E. scabrifolius accessions, one commercial cultivar and seven artificial hybrids of this species. Both types of marker systems resulted in an accurate differentiation of the interrogated E. scabrifolius germplasm in the dendrograms. Moreover, relationships between the tested germplasm tend to vary according to the type of marker system used. Principal Component Analysis was used to identify the traits that best described the morphological variation. The morphological study revealed that almost all hybrids were different from their parents, with exception of one hybrid that was clustered with their own male parent. In the AFLP marker analysis, all the hybrids were clustered with one of their own parents. However, hybrid origin of those individuals was verified by the presence of paternal bands and polymorphic bands. In conclusion, the use of different methodologies to characterize and analyze E. scabrifolius materials has the potential to become of great benefit for the efficient management of germplasm collections and also for their utilization in breeding programs. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas http://hdl.handle.net/20.500.12110/paper_03704661_v45_n2_p85_Tomas
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic AFLP
Elymus
Germplasm identification
Molecular markers
Morphology
spellingShingle AFLP
Elymus
Germplasm identification
Molecular markers
Morphology
Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
topic_facet AFLP
Elymus
Germplasm identification
Molecular markers
Morphology
description Elymus scabrifolius is a South American perennial grass exhibiting great potential as a forage resource, especially for environments with edaphic limitations. In the present work we use morphological traits and AFLP molecular markers to genotypically identify six E. scabrifolius accessions, one commercial cultivar and seven artificial hybrids of this species. Both types of marker systems resulted in an accurate differentiation of the interrogated E. scabrifolius germplasm in the dendrograms. Moreover, relationships between the tested germplasm tend to vary according to the type of marker system used. Principal Component Analysis was used to identify the traits that best described the morphological variation. The morphological study revealed that almost all hybrids were different from their parents, with exception of one hybrid that was clustered with their own male parent. In the AFLP marker analysis, all the hybrids were clustered with one of their own parents. However, hybrid origin of those individuals was verified by the presence of paternal bands and polymorphic bands. In conclusion, the use of different methodologies to characterize and analyze E. scabrifolius materials has the potential to become of great benefit for the efficient management of germplasm collections and also for their utilization in breeding programs.
title Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
title_short Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
title_full Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
title_fullStr Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
title_full_unstemmed Utilization of morphological and AFLP molecular markers in the identification of native and cultivated germplasm of Elymus scabrifolius (Poaceae)
title_sort utilization of morphological and aflp molecular markers in the identification of native and cultivated germplasm of elymus scabrifolius (poaceae)
publishDate 2013
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03704661_v45_n2_p85_Tomas
http://hdl.handle.net/20.500.12110/paper_03704661_v45_n2_p85_Tomas
_version_ 1768543804245147648

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