First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey

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We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU r...

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Autores principales:	Schiaffino, Maria Romina, Izaguirre, Irina
Publicado:	2017
Materias:	Mamiellophyceae Picoplankton Ribosomal genes Southern Patagonia ribosomal spacer DNA ribosome DNA chemistry classification genetics green alga lake metagenomics phylogeny species difference Chlorophyta DNA, Ribosomal DNA, Ribosomal Spacer Lakes Metagenomics Phylogeny Species Specificity
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09324739_v60_n_p45_Lara http://hdl.handle.net/20.500.12110/paper_09324739_v60_n_p45_Lara
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09324739_v60_n_p45_Lara
record_format	dspace
spelling	paper:paper_09324739_v60_n_p45_Lara2023-06-08T15:53:11Z First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey Schiaffino, Maria Romina Izaguirre, Irina Mamiellophyceae Picoplankton Ribosomal genes Southern Patagonia ribosomal spacer DNA ribosome DNA chemistry classification genetics green alga lake metagenomics phylogeny species difference Chlorophyta DNA, Ribosomal DNA, Ribosomal Spacer Lakes Metagenomics Phylogeny Species Specificity We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters. © 2017 Elsevier GmbH Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09324739_v60_n_p45_Lara http://hdl.handle.net/20.500.12110/paper_09324739_v60_n_p45_Lara
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Mamiellophyceae Picoplankton Ribosomal genes Southern Patagonia ribosomal spacer DNA ribosome DNA chemistry classification genetics green alga lake metagenomics phylogeny species difference Chlorophyta DNA, Ribosomal DNA, Ribosomal Spacer Lakes Metagenomics Phylogeny Species Specificity
spellingShingle	Mamiellophyceae Picoplankton Ribosomal genes Southern Patagonia ribosomal spacer DNA ribosome DNA chemistry classification genetics green alga lake metagenomics phylogeny species difference Chlorophyta DNA, Ribosomal DNA, Ribosomal Spacer Lakes Metagenomics Phylogeny Species Specificity Schiaffino, Maria Romina Izaguirre, Irina First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
topic_facet	Mamiellophyceae Picoplankton Ribosomal genes Southern Patagonia ribosomal spacer DNA ribosome DNA chemistry classification genetics green alga lake metagenomics phylogeny species difference Chlorophyta DNA, Ribosomal DNA, Ribosomal Spacer Lakes Metagenomics Phylogeny Species Specificity
description	We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene + ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters. © 2017 Elsevier GmbH
author	Schiaffino, Maria Romina Izaguirre, Irina
author_facet	Schiaffino, Maria Romina Izaguirre, Irina
author_sort	Schiaffino, Maria Romina
title	First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
title_short	First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
title_full	First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
title_fullStr	First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
title_full_unstemmed	First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey
title_sort	first freshwater member ever reported for the family bathycoccaceae (chlorophyta; archaeplastida) from argentinean patagonia revealed by environmental dna survey
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09324739_v60_n_p45_Lara http://hdl.handle.net/20.500.12110/paper_09324739_v60_n_p45_Lara
work_keys_str_mv	AT schiaffinomariaromina firstfreshwatermembereverreportedforthefamilybathycoccaceaechlorophytaarchaeplastidafromargentineanpatagoniarevealedbyenvironmentaldnasurvey AT izaguirreirina firstfreshwatermembereverreportedforthefamilybathycoccaceaechlorophytaarchaeplastidafromargentineanpatagoniarevealedbyenvironmentaldnasurvey
_version_	1768541805429653504

First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey

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