Application of Z-sinapinic matrix in peptide MALDI-MS analysis
Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite a...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v52_n3_p182_Salum http://hdl.handle.net/20.500.12110/paper_10765174_v52_n3_p182_Salum |
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paper:paper_10765174_v52_n3_p182_Salum2023-06-08T16:05:25Z Application of Z-sinapinic matrix in peptide MALDI-MS analysis Giudicessi, Silvana Laura Erra Balsells, Rosa CHCA clusters MALDI-MS peptides Z-sinapinic acid alpha cyano 4 hydroxycinnamic acid angiotensin I angiotensin II bradykinin peptide sinapic acid alpha-cyano-4-hydroxycinnamate cinnamic acid cinnamic acid derivative coumaric acid peptide sinapic acid limit of detection matrix-assisted laser desorption-ionization mass spectrometry molecular model Note peptide analysis peptide synthesis priority journal amino acid sequence chemical phenomena chemistry matrix-assisted laser desorption-ionization mass spectrometry Amino Acid Sequence Cinnamates Coumaric Acids Hydrophobic and Hydrophilic Interactions Limit of Detection Models, Molecular Peptides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd. Fil:Giudicessi, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v52_n3_p182_Salum http://hdl.handle.net/20.500.12110/paper_10765174_v52_n3_p182_Salum |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CHCA clusters MALDI-MS peptides Z-sinapinic acid alpha cyano 4 hydroxycinnamic acid angiotensin I angiotensin II bradykinin peptide sinapic acid alpha-cyano-4-hydroxycinnamate cinnamic acid cinnamic acid derivative coumaric acid peptide sinapic acid limit of detection matrix-assisted laser desorption-ionization mass spectrometry molecular model Note peptide analysis peptide synthesis priority journal amino acid sequence chemical phenomena chemistry matrix-assisted laser desorption-ionization mass spectrometry Amino Acid Sequence Cinnamates Coumaric Acids Hydrophobic and Hydrophilic Interactions Limit of Detection Models, Molecular Peptides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| spellingShingle |
CHCA clusters MALDI-MS peptides Z-sinapinic acid alpha cyano 4 hydroxycinnamic acid angiotensin I angiotensin II bradykinin peptide sinapic acid alpha-cyano-4-hydroxycinnamate cinnamic acid cinnamic acid derivative coumaric acid peptide sinapic acid limit of detection matrix-assisted laser desorption-ionization mass spectrometry molecular model Note peptide analysis peptide synthesis priority journal amino acid sequence chemical phenomena chemistry matrix-assisted laser desorption-ionization mass spectrometry Amino Acid Sequence Cinnamates Coumaric Acids Hydrophobic and Hydrophilic Interactions Limit of Detection Models, Molecular Peptides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Giudicessi, Silvana Laura Erra Balsells, Rosa Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
| topic_facet |
CHCA clusters MALDI-MS peptides Z-sinapinic acid alpha cyano 4 hydroxycinnamic acid angiotensin I angiotensin II bradykinin peptide sinapic acid alpha-cyano-4-hydroxycinnamate cinnamic acid cinnamic acid derivative coumaric acid peptide sinapic acid limit of detection matrix-assisted laser desorption-ionization mass spectrometry molecular model Note peptide analysis peptide synthesis priority journal amino acid sequence chemical phenomena chemistry matrix-assisted laser desorption-ionization mass spectrometry Amino Acid Sequence Cinnamates Coumaric Acids Hydrophobic and Hydrophilic Interactions Limit of Detection Models, Molecular Peptides Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| description |
Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd. |
| author |
Giudicessi, Silvana Laura Erra Balsells, Rosa |
| author_facet |
Giudicessi, Silvana Laura Erra Balsells, Rosa |
| author_sort |
Giudicessi, Silvana Laura |
| title |
Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
| title_short |
Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
| title_full |
Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
| title_fullStr |
Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
| title_full_unstemmed |
Application of Z-sinapinic matrix in peptide MALDI-MS analysis |
| title_sort |
application of z-sinapinic matrix in peptide maldi-ms analysis |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10765174_v52_n3_p182_Salum http://hdl.handle.net/20.500.12110/paper_10765174_v52_n3_p182_Salum |
| work_keys_str_mv |
AT giudicessisilvanalaura applicationofzsinapinicmatrixinpeptidemaldimsanalysis AT errabalsellsrosa applicationofzsinapinicmatrixinpeptidemaldimsanalysis |
| _version_ |
1768544331137810432 |