Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression

The core complex of telomere-associated proteins, named the shelterin complex, plays a critical role in telomere protection and telomere length (TL) homeostasis. In this study, we have explored changes in the expression of telomere-associated genes POT1, TIN2, RAP1 and TPP1, in patients with monoclo...

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Autor principal:	García Rivello, Hernán Jorge
Publicado:	2014
Materias:	MGUS Multiple myeloma POT1 expression Telomere associated genes beta 2 microglobulin calcium pot1 protein protein Rap1 protein shelterin TIN2 protein TPP1 protein unclassified drug article clinical feature gene gene expression gene expression profiling hemostasis human major clinical study monoclonal immunoglobulinemia multiple myeloma overall survival plasma cell plasma cell leukemia POT1 gene priority journal prognosis protein expression RAP1 gene real time polymerase chain reaction restriction fragment telomere TIN2 gene TPP1 gene Aged Aged, 80 and over DNA Methylation Follow-Up Studies Galectin 1 Gene Expression Profiling Humans Middle Aged Monoclonal Gammopathy of Undetermined Significance Multiple Myeloma Prognosis Promoter Regions, Genetic Telomerase Telomere-Binding Proteins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799796_v52_n2-3_p134_Panero http://hdl.handle.net/20.500.12110/paper_10799796_v52_n2-3_p134_Panero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10799796_v52_n2-3_p134_Panero
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spelling	paper:paper_10799796_v52_n2-3_p134_Panero2023-06-08T16:05:38Z Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression García Rivello, Hernán Jorge MGUS Multiple myeloma POT1 expression Telomere associated genes beta 2 microglobulin calcium pot1 protein protein Rap1 protein shelterin TIN2 protein TPP1 protein unclassified drug article clinical feature gene gene expression gene expression profiling hemostasis human major clinical study monoclonal immunoglobulinemia multiple myeloma overall survival plasma cell plasma cell leukemia POT1 gene priority journal prognosis protein expression RAP1 gene real time polymerase chain reaction restriction fragment telomere TIN2 gene TPP1 gene MGUS Multiple myeloma POT1 expression Telomere associated genes Aged Aged, 80 and over DNA Methylation Follow-Up Studies Galectin 1 Gene Expression Profiling Humans Middle Aged Monoclonal Gammopathy of Undetermined Significance Multiple Myeloma Prognosis Promoter Regions, Genetic Telomerase Telomere-Binding Proteins The core complex of telomere-associated proteins, named the shelterin complex, plays a critical role in telomere protection and telomere length (TL) homeostasis. In this study, we have explored changes in the expression of telomere-associated genes POT1, TIN2, RAP1 and TPP1, in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). A total of 154 patients: 70 with MGUS and 84 with MM were studied. Real-time quantitative PCR was used to quantify gene expression. TL was evaluated by Terminal Restriction Fragments. Our data showed increased expression of POT1, TPP1, TIN2 and RAP1 in MM with respect to MGUS patients, with significant differences for POT1 gene (p = 0.002). In MM, the correlation of gene expression profiles with clinical characteristics highlighted POT1 for its significant association with advanced clinical stages, high calcium and β2-microglobulin levels (p = 0.02) and bone lesions (p = 0.009). In multivariate analysis, POT1 expression (p = 0.04) was a significant independent prognostic factor for overall survival as well as the staging system (ISS) (p < 0.02). Our findings suggest for the first time the participation of POT1 in the transformation process from MGUS to MM, and provide evidence of this gene as a useful prognostic factor in MM as well as a possible molecular target to design new therapeutic strategies. © 2013 Elsevier Inc. Fil:García Rivello, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799796_v52_n2-3_p134_Panero http://hdl.handle.net/20.500.12110/paper_10799796_v52_n2-3_p134_Panero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	MGUS Multiple myeloma POT1 expression Telomere associated genes beta 2 microglobulin calcium pot1 protein protein Rap1 protein shelterin TIN2 protein TPP1 protein unclassified drug article clinical feature gene gene expression gene expression profiling hemostasis human major clinical study monoclonal immunoglobulinemia multiple myeloma overall survival plasma cell plasma cell leukemia POT1 gene priority journal prognosis protein expression RAP1 gene real time polymerase chain reaction restriction fragment telomere TIN2 gene TPP1 gene MGUS Multiple myeloma POT1 expression Telomere associated genes Aged Aged, 80 and over DNA Methylation Follow-Up Studies Galectin 1 Gene Expression Profiling Humans Middle Aged Monoclonal Gammopathy of Undetermined Significance Multiple Myeloma Prognosis Promoter Regions, Genetic Telomerase Telomere-Binding Proteins
spellingShingle	MGUS Multiple myeloma POT1 expression Telomere associated genes beta 2 microglobulin calcium pot1 protein protein Rap1 protein shelterin TIN2 protein TPP1 protein unclassified drug article clinical feature gene gene expression gene expression profiling hemostasis human major clinical study monoclonal immunoglobulinemia multiple myeloma overall survival plasma cell plasma cell leukemia POT1 gene priority journal prognosis protein expression RAP1 gene real time polymerase chain reaction restriction fragment telomere TIN2 gene TPP1 gene MGUS Multiple myeloma POT1 expression Telomere associated genes Aged Aged, 80 and over DNA Methylation Follow-Up Studies Galectin 1 Gene Expression Profiling Humans Middle Aged Monoclonal Gammopathy of Undetermined Significance Multiple Myeloma Prognosis Promoter Regions, Genetic Telomerase Telomere-Binding Proteins García Rivello, Hernán Jorge Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
topic_facet	MGUS Multiple myeloma POT1 expression Telomere associated genes beta 2 microglobulin calcium pot1 protein protein Rap1 protein shelterin TIN2 protein TPP1 protein unclassified drug article clinical feature gene gene expression gene expression profiling hemostasis human major clinical study monoclonal immunoglobulinemia multiple myeloma overall survival plasma cell plasma cell leukemia POT1 gene priority journal prognosis protein expression RAP1 gene real time polymerase chain reaction restriction fragment telomere TIN2 gene TPP1 gene MGUS Multiple myeloma POT1 expression Telomere associated genes Aged Aged, 80 and over DNA Methylation Follow-Up Studies Galectin 1 Gene Expression Profiling Humans Middle Aged Monoclonal Gammopathy of Undetermined Significance Multiple Myeloma Prognosis Promoter Regions, Genetic Telomerase Telomere-Binding Proteins
description	The core complex of telomere-associated proteins, named the shelterin complex, plays a critical role in telomere protection and telomere length (TL) homeostasis. In this study, we have explored changes in the expression of telomere-associated genes POT1, TIN2, RAP1 and TPP1, in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). A total of 154 patients: 70 with MGUS and 84 with MM were studied. Real-time quantitative PCR was used to quantify gene expression. TL was evaluated by Terminal Restriction Fragments. Our data showed increased expression of POT1, TPP1, TIN2 and RAP1 in MM with respect to MGUS patients, with significant differences for POT1 gene (p = 0.002). In MM, the correlation of gene expression profiles with clinical characteristics highlighted POT1 for its significant association with advanced clinical stages, high calcium and β2-microglobulin levels (p = 0.02) and bone lesions (p = 0.009). In multivariate analysis, POT1 expression (p = 0.04) was a significant independent prognostic factor for overall survival as well as the staging system (ISS) (p < 0.02). Our findings suggest for the first time the participation of POT1 in the transformation process from MGUS to MM, and provide evidence of this gene as a useful prognostic factor in MM as well as a possible molecular target to design new therapeutic strategies. © 2013 Elsevier Inc.
author	García Rivello, Hernán Jorge
author_facet	García Rivello, Hernán Jorge
author_sort	García Rivello, Hernán Jorge
title	Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_short	Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_full	Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_fullStr	Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_full_unstemmed	Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_sort	expression profile of shelterin components in plasma cell disorders. clinical significance of pot1 overexpression
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10799796_v52_n2-3_p134_Panero http://hdl.handle.net/20.500.12110/paper_10799796_v52_n2-3_p134_Panero
work_keys_str_mv	AT garciarivellohernanjorge expressionprofileofshelterincomponentsinplasmacelldisordersclinicalsignificanceofpot1overexpression
_version_	1768543715792519168

Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression

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