Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium

Murine embryonic stem cells (mESCs) are pluripotent cells that can be propagated in an undifferentiated state in continuous culture on a feeder layer or without feeders in the presence of leukemia inhibitory factor (LIF). Although there has been a great advance since their establishment, ESC culture...

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Autores principales:	Losino, Noelia Ivana, Luzzani, Carlos Daniel, Boffi, Juan Carlos, Louis-Tisserand, Mariana, Barañao, José Lino S., Guberman, Alejandra Sonia
Publicado:	2011
Materias:	kruppel like factor 4 octamer transcription factor 4 Rex protein stage specific embryo antigen 1 transcription factor NANOG transcription factor Sox2 animal cell article cell culture cell differentiation cell growth cell population cell proliferation cell renewal cow culture medium ectoderm embryoid body embryonic stem cell endoderm granulosa cell mesoderm nonhuman pluripotent stem cell priority journal teratoma Animals Biological Markers Cattle Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Culture Media, Conditioned Embryonic Stem Cells Female Granulosa Cells Mice Pluripotent Stem Cells Bovinae Murinae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v20_n8_p1439_Losino http://hdl.handle.net/20.500.12110/paper_15473287_v20_n8_p1439_Losino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15473287_v20_n8_p1439_Losino
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spelling	paper:paper_15473287_v20_n8_p1439_Losino2023-06-08T16:21:17Z Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium Losino, Noelia Ivana Luzzani, Carlos Daniel Boffi, Juan Carlos Louis-Tisserand, Mariana Barañao, José Lino S. Guberman, Alejandra Sonia kruppel like factor 4 octamer transcription factor 4 Rex protein stage specific embryo antigen 1 transcription factor NANOG transcription factor Sox2 animal cell article cell culture cell differentiation cell growth cell population cell proliferation cell renewal cow culture medium ectoderm embryoid body embryonic stem cell endoderm granulosa cell mesoderm nonhuman pluripotent stem cell priority journal teratoma Animals Biological Markers Cattle Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Culture Media, Conditioned Embryonic Stem Cells Female Granulosa Cells Mice Pluripotent Stem Cells Bovinae Murinae Murine embryonic stem cells (mESCs) are pluripotent cells that can be propagated in an undifferentiated state in continuous culture on a feeder layer or without feeders in the presence of leukemia inhibitory factor (LIF). Although there has been a great advance since their establishment, ESC culture is still complex and expensive. Therefore, finding culture conditions that maintain the self-renewal of ESCs, preventing their differentiation and promoting their proliferation, is still an area of great interest. In this work, we studied the effects of the conditioned medium from a bovine granulosa cell line (BGC-CM) on the maintenance of self-renewal and pluripotency of mESCs. We found that this medium is able to maintain mESCs' self-renewal while preserving its critical properties without LIF addition. mESCs cultured in BGC-CM expressed the stem cell markers Oct4, Sox2, Nanog, SSEA-1, Klf4, Rex1, and ECAT1. Moreover, mESCs cultured in BGC-CM gave rise to embryoid bodies and teratomas that differentiated effectively to diverse cell populations from endoderm, mesoderm, and ectoderm. Further, we found that mESCs cultured in BGC-CM have an increased proliferation rate compared with cells grown in the mESC standard culture medium supplemented with LIF. These findings may provide a powerful tool to culture mESCs for long periods of time with high proliferation rate while preserving its basic characteristics, contributing to the application of these cells to assess potential tissue engineering and cellular therapy applications. © Copyright 2011, Mary Ann Liebert, Inc. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Boffi, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tisserand, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v20_n8_p1439_Losino http://hdl.handle.net/20.500.12110/paper_15473287_v20_n8_p1439_Losino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	kruppel like factor 4 octamer transcription factor 4 Rex protein stage specific embryo antigen 1 transcription factor NANOG transcription factor Sox2 animal cell article cell culture cell differentiation cell growth cell population cell proliferation cell renewal cow culture medium ectoderm embryoid body embryonic stem cell endoderm granulosa cell mesoderm nonhuman pluripotent stem cell priority journal teratoma Animals Biological Markers Cattle Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Culture Media, Conditioned Embryonic Stem Cells Female Granulosa Cells Mice Pluripotent Stem Cells Bovinae Murinae
spellingShingle	kruppel like factor 4 octamer transcription factor 4 Rex protein stage specific embryo antigen 1 transcription factor NANOG transcription factor Sox2 animal cell article cell culture cell differentiation cell growth cell population cell proliferation cell renewal cow culture medium ectoderm embryoid body embryonic stem cell endoderm granulosa cell mesoderm nonhuman pluripotent stem cell priority journal teratoma Animals Biological Markers Cattle Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Culture Media, Conditioned Embryonic Stem Cells Female Granulosa Cells Mice Pluripotent Stem Cells Bovinae Murinae Losino, Noelia Ivana Luzzani, Carlos Daniel Boffi, Juan Carlos Louis-Tisserand, Mariana Barañao, José Lino S. Guberman, Alejandra Sonia Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
topic_facet	kruppel like factor 4 octamer transcription factor 4 Rex protein stage specific embryo antigen 1 transcription factor NANOG transcription factor Sox2 animal cell article cell culture cell differentiation cell growth cell population cell proliferation cell renewal cow culture medium ectoderm embryoid body embryonic stem cell endoderm granulosa cell mesoderm nonhuman pluripotent stem cell priority journal teratoma Animals Biological Markers Cattle Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Culture Media, Conditioned Embryonic Stem Cells Female Granulosa Cells Mice Pluripotent Stem Cells Bovinae Murinae
description	Murine embryonic stem cells (mESCs) are pluripotent cells that can be propagated in an undifferentiated state in continuous culture on a feeder layer or without feeders in the presence of leukemia inhibitory factor (LIF). Although there has been a great advance since their establishment, ESC culture is still complex and expensive. Therefore, finding culture conditions that maintain the self-renewal of ESCs, preventing their differentiation and promoting their proliferation, is still an area of great interest. In this work, we studied the effects of the conditioned medium from a bovine granulosa cell line (BGC-CM) on the maintenance of self-renewal and pluripotency of mESCs. We found that this medium is able to maintain mESCs' self-renewal while preserving its critical properties without LIF addition. mESCs cultured in BGC-CM expressed the stem cell markers Oct4, Sox2, Nanog, SSEA-1, Klf4, Rex1, and ECAT1. Moreover, mESCs cultured in BGC-CM gave rise to embryoid bodies and teratomas that differentiated effectively to diverse cell populations from endoderm, mesoderm, and ectoderm. Further, we found that mESCs cultured in BGC-CM have an increased proliferation rate compared with cells grown in the mESC standard culture medium supplemented with LIF. These findings may provide a powerful tool to culture mESCs for long periods of time with high proliferation rate while preserving its basic characteristics, contributing to the application of these cells to assess potential tissue engineering and cellular therapy applications. © Copyright 2011, Mary Ann Liebert, Inc.
author	Losino, Noelia Ivana Luzzani, Carlos Daniel Boffi, Juan Carlos Louis-Tisserand, Mariana Barañao, José Lino S. Guberman, Alejandra Sonia
author_facet	Losino, Noelia Ivana Luzzani, Carlos Daniel Boffi, Juan Carlos Louis-Tisserand, Mariana Barañao, José Lino S. Guberman, Alejandra Sonia
author_sort	Losino, Noelia Ivana
title	Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
title_short	Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
title_full	Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
title_fullStr	Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
title_full_unstemmed	Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
title_sort	maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15473287_v20_n8_p1439_Losino http://hdl.handle.net/20.500.12110/paper_15473287_v20_n8_p1439_Losino
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_version_	1768544010184425472

Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium

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