Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis
Embryogenesis relies on instructions provided by spatially organized signaling molecules known as morphogens. Understanding the principles behind morphogen distribution and how cells interpret locally this information remains a major challenge in developmental biology. Here, we introduce morphogen-a...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17444292_v14_n9_p_Durrieu http://hdl.handle.net/20.500.12110/paper_17444292_v14_n9_p_Durrieu |
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paper:paper_17444292_v14_n9_p_Durrieu2023-06-08T16:28:12Z Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis Drosophila melanogaster embryogenesis fluorescent timers morphogen gradient SPIM morphogen protein Article diffusion coefficient Drosophila embryo embryo development model molecular dynamics nonhuman priority journal protein analysis protein degradation protein processing protein synthesis quantitative analysis signal transduction Embryogenesis relies on instructions provided by spatially organized signaling molecules known as morphogens. Understanding the principles behind morphogen distribution and how cells interpret locally this information remains a major challenge in developmental biology. Here, we introduce morphogen-age measurements as a novel approach to test models of morphogen gradient formation. Using a tandem fluorescent timer as a protein age sensor, we find a gradient of increasing age of Bicoid along the anterior–posterior axis in the early Drosophila embryo. Quantitative analysis of the protein age distribution across the embryo reveals that the synthesis–diffusion–degradation model is the most likely model underlying Bicoid gradient formation, and rules out other hypotheses for gradient formation. Moreover, we show that the timer can detect transitions in the dynamics associated with syncytial cellularization. Our results provide new insight into Bicoid gradient formation and demonstrate how morphogen-age information can complement knowledge about movement, abundance, and distribution, which should be widely applicable to other systems. © 2018 The Authors. Published under the terms of the CC BY 4.0 license 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17444292_v14_n9_p_Durrieu http://hdl.handle.net/20.500.12110/paper_17444292_v14_n9_p_Durrieu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Drosophila melanogaster embryogenesis fluorescent timers morphogen gradient SPIM morphogen protein Article diffusion coefficient Drosophila embryo embryo development model molecular dynamics nonhuman priority journal protein analysis protein degradation protein processing protein synthesis quantitative analysis signal transduction |
| spellingShingle |
Drosophila melanogaster embryogenesis fluorescent timers morphogen gradient SPIM morphogen protein Article diffusion coefficient Drosophila embryo embryo development model molecular dynamics nonhuman priority journal protein analysis protein degradation protein processing protein synthesis quantitative analysis signal transduction Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| topic_facet |
Drosophila melanogaster embryogenesis fluorescent timers morphogen gradient SPIM morphogen protein Article diffusion coefficient Drosophila embryo embryo development model molecular dynamics nonhuman priority journal protein analysis protein degradation protein processing protein synthesis quantitative analysis signal transduction |
| description |
Embryogenesis relies on instructions provided by spatially organized signaling molecules known as morphogens. Understanding the principles behind morphogen distribution and how cells interpret locally this information remains a major challenge in developmental biology. Here, we introduce morphogen-age measurements as a novel approach to test models of morphogen gradient formation. Using a tandem fluorescent timer as a protein age sensor, we find a gradient of increasing age of Bicoid along the anterior–posterior axis in the early Drosophila embryo. Quantitative analysis of the protein age distribution across the embryo reveals that the synthesis–diffusion–degradation model is the most likely model underlying Bicoid gradient formation, and rules out other hypotheses for gradient formation. Moreover, we show that the timer can detect transitions in the dynamics associated with syncytial cellularization. Our results provide new insight into Bicoid gradient formation and demonstrate how morphogen-age information can complement knowledge about movement, abundance, and distribution, which should be widely applicable to other systems. © 2018 The Authors. Published under the terms of the CC BY 4.0 license |
| title |
Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| title_short |
Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| title_full |
Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| title_fullStr |
Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| title_full_unstemmed |
Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| title_sort |
bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17444292_v14_n9_p_Durrieu http://hdl.handle.net/20.500.12110/paper_17444292_v14_n9_p_Durrieu |
| _version_ |
1768541818531610624 |