FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells

The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh...

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Autores principales:	Correa García, Susana Raquel, Bermudez Moretti, Mariana
Publicado:	2014
Materias:	Biological medium Freeze-drying process FT-IR spectrum Growth conditions Metabolome analysis Multivariate data Saccharomyces cerevisiae yeast Yeast Saccharomyces cerevisiae Cells Cytology Data processing Digital storage Fourier transform infrared spectroscopy Spectroscopic analysis Yeast
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17599660_v6_n6_p1855_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_17599660_v6_n6_p1855_CorreaGarcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_17599660_v6_n6_p1855_CorreaGarcia
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spelling	paper:paper_17599660_v6_n6_p1855_CorreaGarcia2023-06-08T16:29:03Z FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells Correa García, Susana Raquel Bermudez Moretti, Mariana Biological medium Freeze-drying process FT-IR spectrum Growth conditions Metabolome analysis Multivariate data Saccharomyces cerevisiae yeast Yeast Saccharomyces cerevisiae Cells Cytology Data processing Digital storage Fourier transform infrared spectroscopy Spectroscopic analysis Yeast The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability. © The Royal Society of Chemistry 2014. Fil:Correa-García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bermúdez-Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17599660_v6_n6_p1855_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_17599660_v6_n6_p1855_CorreaGarcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biological medium Freeze-drying process FT-IR spectrum Growth conditions Metabolome analysis Multivariate data Saccharomyces cerevisiae yeast Yeast Saccharomyces cerevisiae Cells Cytology Data processing Digital storage Fourier transform infrared spectroscopy Spectroscopic analysis Yeast
spellingShingle	Biological medium Freeze-drying process FT-IR spectrum Growth conditions Metabolome analysis Multivariate data Saccharomyces cerevisiae yeast Yeast Saccharomyces cerevisiae Cells Cytology Data processing Digital storage Fourier transform infrared spectroscopy Spectroscopic analysis Yeast Correa García, Susana Raquel Bermudez Moretti, Mariana FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
topic_facet	Biological medium Freeze-drying process FT-IR spectrum Growth conditions Metabolome analysis Multivariate data Saccharomyces cerevisiae yeast Yeast Saccharomyces cerevisiae Cells Cytology Data processing Digital storage Fourier transform infrared spectroscopy Spectroscopic analysis Yeast
description	The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability. © The Royal Society of Chemistry 2014.
author	Correa García, Susana Raquel Bermudez Moretti, Mariana
author_facet	Correa García, Susana Raquel Bermudez Moretti, Mariana
author_sort	Correa García, Susana Raquel
title	FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_short	FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_full	FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_fullStr	FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_full_unstemmed	FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_sort	ftir spectroscopic metabolome analysis of lyophilized and fresh saccharomyces cerevisiae yeast cells
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17599660_v6_n6_p1855_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_17599660_v6_n6_p1855_CorreaGarcia
work_keys_str_mv	AT correagarciasusanaraquel ftirspectroscopicmetabolomeanalysisoflyophilizedandfreshsaccharomycescerevisiaeyeastcells AT bermudezmorettimariana ftirspectroscopicmetabolomeanalysisoflyophilizedandfreshsaccharomycescerevisiaeyeastcells
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FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells

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