Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1

In the present study, the antiviral properties of the bacteriocin subtilosin against Herpes simplex virus type 1 (HSV-1) and the safety and efficacy of a subtilosin-based nanofiber formulation were determined. High concentrations of subtilosin, the cyclical antimicrobial peptide produced by Bacillus...

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Autores principales:	Torres, Nicolás I., Wachsman, Mónica Beatriz
Publicado:	2013
Materias:	Antiviral Bacteriocin Nanofiber Subtilosin aciclovir foscarnet nanofiber polypeptide antibiotic agent polyvinyl alcohol subtilosin unclassified drug agar diffusion animal cell antiviral activity article Bacillus amyloliquefaciens concentration response controlled study drug cytotoxicity drug delivery system drug efficacy drug formulation drug safety electrospinning epidermis Herpes simplex virus 1 human human tissue in vitro study nonhuman priority journal protein synthesis Vero cell virogenesis virus inhibition virus mutant Western blotting wild type Human herpesvirus 1
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18671306_v5_n1_p26_Torres http://hdl.handle.net/20.500.12110/paper_18671306_v5_n1_p26_Torres
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_18671306_v5_n1_p26_Torres
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spelling	paper:paper_18671306_v5_n1_p26_Torres2023-06-08T16:29:48Z Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1 Torres, Nicolás I. Wachsman, Mónica Beatriz Antiviral Bacteriocin Nanofiber Subtilosin aciclovir foscarnet nanofiber polypeptide antibiotic agent polyvinyl alcohol subtilosin unclassified drug agar diffusion animal cell antiviral activity article Bacillus amyloliquefaciens concentration response controlled study drug cytotoxicity drug delivery system drug efficacy drug formulation drug safety electrospinning epidermis Herpes simplex virus 1 human human tissue in vitro study nonhuman priority journal protein synthesis Vero cell virogenesis virus inhibition virus mutant Western blotting wild type Bacillus amyloliquefaciens Human herpesvirus 1 In the present study, the antiviral properties of the bacteriocin subtilosin against Herpes simplex virus type 1 (HSV-1) and the safety and efficacy of a subtilosin-based nanofiber formulation were determined. High concentrations of subtilosin, the cyclical antimicrobial peptide produced by Bacillus amyloliquefaciens, were virucidal against HSV-1. Interestingly, at non-virucidal concentrations, subtilosin inhibited wild type HSV-1 and aciclovir-resistant mutants in a dose-dependent manner. Although the exact antiviral mechanism is not fully understood, time of addition experiments and western blot analysis suggest that subtilosin does not affect viral multiplication steps prior to protein synthesis. Poly(vinyl alcohol)-based subtilosin nanofibers with a width of 278 nm were produced by the electrospinning process. The retained antimicrobial activity of the subtilosin-based fibers was determined via an agar well diffusion assay. The loading capacity of the fibers was 2. 4 mg subtilosin/g fiber, and loading efficiency was 31. 6 %. Furthermore, the nanofibers with and without incorporated subtilosin were shown to be non-toxic to human epidermal tissues using an in vitro human tissue model. Taking together these results, subtilosin-based nanofibers should be further studied as a novel alternative method for treatment and/or control of HSV-1 infection. © 2013 Springer Science+Business Media New York. Fil:Torres, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18671306_v5_n1_p26_Torres http://hdl.handle.net/20.500.12110/paper_18671306_v5_n1_p26_Torres
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral Bacteriocin Nanofiber Subtilosin aciclovir foscarnet nanofiber polypeptide antibiotic agent polyvinyl alcohol subtilosin unclassified drug agar diffusion animal cell antiviral activity article Bacillus amyloliquefaciens concentration response controlled study drug cytotoxicity drug delivery system drug efficacy drug formulation drug safety electrospinning epidermis Herpes simplex virus 1 human human tissue in vitro study nonhuman priority journal protein synthesis Vero cell virogenesis virus inhibition virus mutant Western blotting wild type Bacillus amyloliquefaciens Human herpesvirus 1
spellingShingle	Antiviral Bacteriocin Nanofiber Subtilosin aciclovir foscarnet nanofiber polypeptide antibiotic agent polyvinyl alcohol subtilosin unclassified drug agar diffusion animal cell antiviral activity article Bacillus amyloliquefaciens concentration response controlled study drug cytotoxicity drug delivery system drug efficacy drug formulation drug safety electrospinning epidermis Herpes simplex virus 1 human human tissue in vitro study nonhuman priority journal protein synthesis Vero cell virogenesis virus inhibition virus mutant Western blotting wild type Bacillus amyloliquefaciens Human herpesvirus 1 Torres, Nicolás I. Wachsman, Mónica Beatriz Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1
topic_facet	Antiviral Bacteriocin Nanofiber Subtilosin aciclovir foscarnet nanofiber polypeptide antibiotic agent polyvinyl alcohol subtilosin unclassified drug agar diffusion animal cell antiviral activity article Bacillus amyloliquefaciens concentration response controlled study drug cytotoxicity drug delivery system drug efficacy drug formulation drug safety electrospinning epidermis Herpes simplex virus 1 human human tissue in vitro study nonhuman priority journal protein synthesis Vero cell virogenesis virus inhibition virus mutant Western blotting wild type Bacillus amyloliquefaciens Human herpesvirus 1
description	In the present study, the antiviral properties of the bacteriocin subtilosin against Herpes simplex virus type 1 (HSV-1) and the safety and efficacy of a subtilosin-based nanofiber formulation were determined. High concentrations of subtilosin, the cyclical antimicrobial peptide produced by Bacillus amyloliquefaciens, were virucidal against HSV-1. Interestingly, at non-virucidal concentrations, subtilosin inhibited wild type HSV-1 and aciclovir-resistant mutants in a dose-dependent manner. Although the exact antiviral mechanism is not fully understood, time of addition experiments and western blot analysis suggest that subtilosin does not affect viral multiplication steps prior to protein synthesis. Poly(vinyl alcohol)-based subtilosin nanofibers with a width of 278 nm were produced by the electrospinning process. The retained antimicrobial activity of the subtilosin-based fibers was determined via an agar well diffusion assay. The loading capacity of the fibers was 2. 4 mg subtilosin/g fiber, and loading efficiency was 31. 6 %. Furthermore, the nanofibers with and without incorporated subtilosin were shown to be non-toxic to human epidermal tissues using an in vitro human tissue model. Taking together these results, subtilosin-based nanofibers should be further studied as a novel alternative method for treatment and/or control of HSV-1 infection. © 2013 Springer Science+Business Media New York.
author	Torres, Nicolás I. Wachsman, Mónica Beatriz
author_facet	Torres, Nicolás I. Wachsman, Mónica Beatriz
author_sort	Torres, Nicolás I.
title	Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1
title_short	Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1
title_full	Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1
title_fullStr	Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1
title_full_unstemmed	Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1
title_sort	safety, formulation and in vitro antiviral activity of the antimicrobial peptide subtilosin against herpes simplex virus type 1
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18671306_v5_n1_p26_Torres http://hdl.handle.net/20.500.12110/paper_18671306_v5_n1_p26_Torres
work_keys_str_mv	AT torresnicolasi safetyformulationandinvitroantiviralactivityoftheantimicrobialpeptidesubtilosinagainstherpessimplexvirustype1 AT wachsmanmonicabeatriz safetyformulationandinvitroantiviralactivityoftheantimicrobialpeptidesubtilosinagainstherpessimplexvirustype1
_version_	1768543724896256000

Safety, Formulation and In Vitro Antiviral Activity of the Antimicrobial Peptide Subtilosin Against Herpes Simplex Virus Type 1

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