Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals

Bioprocesses conducted under conditions with restricted O2 supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence, has elaborate sensing and signal transduction...

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Autores principales: Ruiz, Jimena Alicia, de Almeida, Alejandra, Méndez, Beatriz Silvia
Publicado: 2012
Materias:
ArcBA
CreBC
Escherichia coli
Metabolic flux analysis
Polyhydroxyalkanoates
Redox homeostasis
Reduced biochemicals
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20010370_v3_n4_pe201210019_Ruiz
http://hdl.handle.net/20.500.12110/paper_20010370_v3_n4_pe201210019_Ruiz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_20010370_v3_n4_pe201210019_Ruiz
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spelling paper:paper_20010370_v3_n4_pe201210019_Ruiz2023-06-08T16:32:55Z Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals Ruiz, Jimena Alicia de Almeida, Alejandra Méndez, Beatriz Silvia ArcBA CreBC Escherichia coli Metabolic flux analysis Polyhydroxyalkanoates Redox homeostasis Reduced biochemicals Bioprocesses conducted under conditions with restricted O2 supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence, has elaborate sensing and signal transduction mechanisms that respond to the availability of electron acceptors and alternative carbon sources in the surrounding environment. In particular, the ArcBA and CreBC two-component signal transduction systems are largely responsible for the metabolic regulation of redox control in response to O2 availability and carbon source utilization, respectively. Significant advances in the understanding of the biochemical, genetic, and physiological duties of these regulatory systems have been achieved in recent years. This situation allowed to rationally-design novel engineering approaches that ensure optimal carbon and energy flows within central metabolism, as well as to manipulate redox homeostasis, in order to optimize the production of industrially-relevant metabolites. In particular, metabolic flux analysis provided new clues to understand the metabolic regulation mediated by the ArcBA and CreBC systems. Genetic manipulation of these regulators proved useful for designing microbial cells factories tailored for the synthesis of reduced biochemicals with added value, such as poly(3-hydroxybutyrate), under conditions with restricted O2 supply. This network-wide strategy is in contrast with traditional metabolic engineering approaches, that entail direct modification of the pathway(s) at stake, and opens new avenues for the targeted modulation of central catabolic pathways at the transcriptional level. © 2012 Bernstein and Carlson. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20010370_v3_n4_pe201210019_Ruiz http://hdl.handle.net/20.500.12110/paper_20010370_v3_n4_pe201210019_Ruiz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic ArcBA
CreBC
Escherichia coli
Metabolic flux analysis
Polyhydroxyalkanoates
Redox homeostasis
Reduced biochemicals
spellingShingle ArcBA
CreBC
Escherichia coli
Metabolic flux analysis
Polyhydroxyalkanoates
Redox homeostasis
Reduced biochemicals
Ruiz, Jimena Alicia
de Almeida, Alejandra
Méndez, Beatriz Silvia
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
topic_facet ArcBA
CreBC
Escherichia coli
Metabolic flux analysis
Polyhydroxyalkanoates
Redox homeostasis
Reduced biochemicals
description Bioprocesses conducted under conditions with restricted O2 supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence, has elaborate sensing and signal transduction mechanisms that respond to the availability of electron acceptors and alternative carbon sources in the surrounding environment. In particular, the ArcBA and CreBC two-component signal transduction systems are largely responsible for the metabolic regulation of redox control in response to O2 availability and carbon source utilization, respectively. Significant advances in the understanding of the biochemical, genetic, and physiological duties of these regulatory systems have been achieved in recent years. This situation allowed to rationally-design novel engineering approaches that ensure optimal carbon and energy flows within central metabolism, as well as to manipulate redox homeostasis, in order to optimize the production of industrially-relevant metabolites. In particular, metabolic flux analysis provided new clues to understand the metabolic regulation mediated by the ArcBA and CreBC systems. Genetic manipulation of these regulators proved useful for designing microbial cells factories tailored for the synthesis of reduced biochemicals with added value, such as poly(3-hydroxybutyrate), under conditions with restricted O2 supply. This network-wide strategy is in contrast with traditional metabolic engineering approaches, that entail direct modification of the pathway(s) at stake, and opens new avenues for the targeted modulation of central catabolic pathways at the transcriptional level. © 2012 Bernstein and Carlson.
author Ruiz, Jimena Alicia
de Almeida, Alejandra
Méndez, Beatriz Silvia
author_facet Ruiz, Jimena Alicia
de Almeida, Alejandra
Méndez, Beatriz Silvia
author_sort Ruiz, Jimena Alicia
title Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
title_short Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
title_full Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
title_fullStr Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
title_full_unstemmed Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
title_sort escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20010370_v3_n4_pe201210019_Ruiz
http://hdl.handle.net/20.500.12110/paper_20010370_v3_n4_pe201210019_Ruiz
work_keys_str_mv AT ruizjimenaalicia escherichiacoliredoxmutantsasmicrobialcellfactoriesforthesynthesisofreducedbiochemicals
AT dealmeidaalejandra escherichiacoliredoxmutantsasmicrobialcellfactoriesforthesynthesisofreducedbiochemicals
AT mendezbeatrizsilvia escherichiacoliredoxmutantsasmicrobialcellfactoriesforthesynthesisofreducedbiochemicals
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