Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells

Mostrar todas las versiones(3)

Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana susp...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zhang, Z., Ramirez, J., Reboutier, D., Brault, M., Trouverie, J., Pennarun, A.-M., Amiar, Z., Biligui, B., Galagovsky, L., Rona, J.-P.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2005
Materias:
Arabidopsis thaliana
Brassinosteroid
Ion currents
Plasma membrane
Proton pump
Suspension cells
anion
ion channel
proton pump
steroid
Arabidopsis
article
cell membrane
cytology
metabolism
physiology
Anions
Cell Membrane
Ion Channels
Proton Pumps
Steroids
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_00320781_v46_n9_p1494_Zhang
record_format dspace
spelling paperaa:paper_00320781_v46_n9_p1494_Zhang2023-06-12T16:45:26Z Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells Plant Cell Physiol. 2005;46(9):1494-1504 Zhang, Z. Ramirez, J. Reboutier, D. Brault, M. Trouverie, J. Pennarun, A.-M. Amiar, Z. Biligui, B. Galagovsky, L. Rona, J.-P. Arabidopsis thaliana Brassinosteroid Ion currents Plasma membrane Proton pump Suspension cells anion ion channel proton pump steroid Arabidopsis article cell membrane cytology metabolism physiology Anions Arabidopsis Cell Membrane Ion Channels Proton Pumps Steroids Arabidopsis Arabidopsis thaliana Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity. © 2005 JSPP. Fil:Ramirez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Arabidopsis thaliana
Brassinosteroid
Ion currents
Plasma membrane
Proton pump
Suspension cells
anion
ion channel
proton pump
steroid
Arabidopsis
article
cell membrane
cytology
metabolism
physiology
Anions
Arabidopsis
Cell Membrane
Ion Channels
Proton Pumps
Steroids
Arabidopsis
Arabidopsis thaliana
spellingShingle Arabidopsis thaliana
Brassinosteroid
Ion currents
Plasma membrane
Proton pump
Suspension cells
anion
ion channel
proton pump
steroid
Arabidopsis
article
cell membrane
cytology
metabolism
physiology
Anions
Arabidopsis
Cell Membrane
Ion Channels
Proton Pumps
Steroids
Arabidopsis
Arabidopsis thaliana
Zhang, Z.
Ramirez, J.
Reboutier, D.
Brault, M.
Trouverie, J.
Pennarun, A.-M.
Amiar, Z.
Biligui, B.
Galagovsky, L.
Rona, J.-P.
Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
topic_facet Arabidopsis thaliana
Brassinosteroid
Ion currents
Plasma membrane
Proton pump
Suspension cells
anion
ion channel
proton pump
steroid
Arabidopsis
article
cell membrane
cytology
metabolism
physiology
Anions
Arabidopsis
Cell Membrane
Ion Channels
Proton Pumps
Steroids
Arabidopsis
Arabidopsis thaliana
description Brassinosteroids (BRs) are involved in numerous physiological processes associated with plant development and especially with cell expansion. Here we report that two BRs, 28-homobrassinolide (HBL) and its direct precursor 28-homocastasterone (HCS), promote cell expansion of Arabidopsis thaliana suspension cells. We also show that cell expansions induced by HBL and HCS are correlated with the amplitude of the plasma membrane hyperpolarization they elicited. HBL, which promoted the larger cell expansion, also provoked the larger hyperpolarization. We observed that membrane hyperpolarization and cell expansion were partially inhibited by the proton pump inhibitor erythrosin B, suggesting that proton pumps were not the only ion transport system modulated by the two BRs. We used a voltage clamp approach in order to find the other ion transport systems involved in the PM hyperpolarization elicited by HBL and HCS. Interestingly, while anion currents were inhibited by both HBL and HCS, outward rectifying K+ currents were increased by HBL but inhibited by HCS. The different electrophysiological behavior shown by HBL and HCS indicates that small changes in the BR skeleton might be responsible for changes in bioactivity. © 2005 JSPP.
format Artículo
Artículo
publishedVersion
author Zhang, Z.
Ramirez, J.
Reboutier, D.
Brault, M.
Trouverie, J.
Pennarun, A.-M.
Amiar, Z.
Biligui, B.
Galagovsky, L.
Rona, J.-P.
author_facet Zhang, Z.
Ramirez, J.
Reboutier, D.
Brault, M.
Trouverie, J.
Pennarun, A.-M.
Amiar, Z.
Biligui, B.
Galagovsky, L.
Rona, J.-P.
author_sort Zhang, Z.
title Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_short Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_full Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_fullStr Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_full_unstemmed Brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of Arabidopsis thaliana cells
title_sort brassinosteroids regulate plasma membrane anion channels in addition to proton pumps during expansion of arabidopsis thaliana cells
publishDate 2005
url http://hdl.handle.net/20.500.12110/paper_00320781_v46_n9_p1494_Zhang
work_keys_str_mv AT zhangz brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT ramirezj brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT reboutierd brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT braultm brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT trouveriej brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT pennarunam brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT amiarz brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT biliguib brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT galagovskyl brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
AT ronajp brassinosteroidsregulateplasmamembraneanionchannelsinadditiontoprotonpumpsduringexpansionofarabidopsisthalianacells
_version_ 1769810280499380224

Ejemplares similares